Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17850 and BWI76_RS17855 overlap by 1 nucleotides BWI76_RS17855 and BWI76_RS17860 are separated by 1 nucleotides BWI76_RS17860 and BWI76_RS17865 are separated by 90 nucleotides
BWI76_RS17850: BWI76_RS17850 - alginate lyase, at 3,710,835 to 3,712,073
_RS17850
BWI76_RS17855: BWI76_RS17855 - alginate lyase, at 3,712,073 to 3,714,103
_RS17855
BWI76_RS17860: BWI76_RS17860 - YoaH family protein, at 3,714,105 to 3,714,284
_RS17860
BWI76_RS17865: BWI76_RS17865 - aminodeoxychorismate synthase subunit I, at 3,714,375 to 3,715,730
_RS17865
Position (kb)
3712
3713
3714
3715 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3711.132 kb on + strand, within BWI76_RS17850 at 3711.133 kb on - strand, within BWI76_RS17850 at 3711.135 kb on - strand, within BWI76_RS17850 at 3711.142 kb on + strand, within BWI76_RS17850 at 3711.308 kb on + strand, within BWI76_RS17850 at 3711.310 kb on - strand, within BWI76_RS17850 at 3711.427 kb on - strand, within BWI76_RS17850 at 3711.460 kb on - strand, within BWI76_RS17850 at 3711.487 kb on + strand at 3711.541 kb on + strand, within BWI76_RS17850 at 3711.756 kb on + strand, within BWI76_RS17850 at 3711.831 kb on - strand, within BWI76_RS17850 at 3711.848 kb on - strand, within BWI76_RS17850 at 3712.068 kb on - strand at 3712.250 kb on - strand at 3712.456 kb on + strand, within BWI76_RS17855 at 3712.484 kb on + strand, within BWI76_RS17855 at 3712.570 kb on - strand, within BWI76_RS17855 at 3712.622 kb on + strand, within BWI76_RS17855 at 3712.832 kb on - strand, within BWI76_RS17855 at 3712.936 kb on - strand, within BWI76_RS17855 at 3713.040 kb on + strand, within BWI76_RS17855 at 3713.394 kb on + strand, within BWI76_RS17855 at 3713.514 kb on + strand, within BWI76_RS17855 at 3713.580 kb on + strand, within BWI76_RS17855 at 3713.800 kb on + strand, within BWI76_RS17855 at 3714.075 kb on + strand at 3714.094 kb on - strand at 3714.194 kb on - strand, within BWI76_RS17860 at 3714.261 kb on - strand at 3714.368 kb on - strand at 3714.368 kb on - strand at 3714.445 kb on + strand at 3714.452 kb on + strand at 3714.618 kb on + strand, within BWI76_RS17865 at 3714.642 kb on - strand, within BWI76_RS17865 at 3714.906 kb on - strand, within BWI76_RS17865 at 3714.927 kb on + strand, within BWI76_RS17865 at 3714.965 kb on + strand, within BWI76_RS17865 at 3714.974 kb on - strand, within BWI76_RS17865 at 3714.975 kb on + strand, within BWI76_RS17865
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 3,711,132 + BWI76_RS17850 0.24 -1.3 3,711,133 - BWI76_RS17850 0.24 -1.8 3,711,135 - BWI76_RS17850 0.24 -2.0 3,711,142 + BWI76_RS17850 0.25 +0.8 3,711,308 + BWI76_RS17850 0.38 -0.4 3,711,310 - BWI76_RS17850 0.38 -0.1 3,711,427 - BWI76_RS17850 0.48 -0.9 3,711,460 - BWI76_RS17850 0.50 -0.1 3,711,487 + +1.9 3,711,541 + BWI76_RS17850 0.57 +0.3 3,711,756 + BWI76_RS17850 0.74 +0.1 3,711,831 - BWI76_RS17850 0.80 -0.5 3,711,848 - BWI76_RS17850 0.82 +0.5 3,712,068 - -0.5 3,712,250 - +1.4 3,712,456 + BWI76_RS17855 0.19 -0.1 3,712,484 + BWI76_RS17855 0.20 +1.1 3,712,570 - BWI76_RS17855 0.24 +1.9 3,712,622 + BWI76_RS17855 0.27 +1.7 3,712,832 - BWI76_RS17855 0.37 +0.0 3,712,936 - BWI76_RS17855 0.42 +0.1 3,713,040 + BWI76_RS17855 0.48 -0.5 3,713,394 + BWI76_RS17855 0.65 +2.8 3,713,514 + BWI76_RS17855 0.71 +4.9 3,713,580 + BWI76_RS17855 0.74 +1.2 3,713,800 + BWI76_RS17855 0.85 -1.6 3,714,075 + -0.7 3,714,094 - -0.5 3,714,194 - BWI76_RS17860 0.49 +0.8 3,714,261 - +3.5 3,714,368 - -0.8 3,714,368 - -1.0 3,714,445 + -0.1 3,714,452 + +0.0 3,714,618 + BWI76_RS17865 0.18 -2.3 3,714,642 - BWI76_RS17865 0.20 +0.3 3,714,906 - BWI76_RS17865 0.39 -0.4 3,714,927 + BWI76_RS17865 0.41 +0.3 3,714,965 + BWI76_RS17865 0.44 +1.9 3,714,974 - BWI76_RS17865 0.44 -0.8 3,714,975 + BWI76_RS17865 0.44 +1.6
Or see this region's nucleotide sequence