Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17750 and BWI76_RS17755 are separated by 130 nucleotides BWI76_RS17755 and BWI76_RS17760 are separated by 118 nucleotides BWI76_RS17760 and BWI76_RS17765 are separated by 8 nucleotides BWI76_RS17765 and BWI76_RS17770 are separated by 94 nucleotides
BWI76_RS17750: BWI76_RS17750 - Na+/H+ antiporter NhaB, at 3,691,745 to 3,693,298
_RS17750
BWI76_RS17755: BWI76_RS17755 - disulfide bond formation protein B, at 3,693,429 to 3,693,959
_RS17755
BWI76_RS17760: BWI76_RS17760 - hypothetical protein, at 3,694,078 to 3,694,422
_RS17760
BWI76_RS17765: BWI76_RS17765 - hypothetical protein, at 3,694,431 to 3,694,877
_RS17765
BWI76_RS17770: BWI76_RS17770 - isomerase/hydrolase, at 3,694,972 to 3,695,631
_RS17770
Position (kb)
3694
3695 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3693.189 kb on + strand at 3693.397 kb on + strand at 3693.420 kb on - strand at 3693.451 kb on + strand at 3693.898 kb on + strand, within BWI76_RS17755 at 3694.273 kb on + strand, within BWI76_RS17760 at 3694.333 kb on - strand, within BWI76_RS17760 at 3694.611 kb on - strand, within BWI76_RS17765 at 3694.655 kb on - strand, within BWI76_RS17765 at 3694.948 kb on + strand at 3694.977 kb on + strand at 3695.186 kb on - strand, within BWI76_RS17770 at 3695.420 kb on + strand, within BWI76_RS17770
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 3,693,189 + +1.4 3,693,397 + -0.7 3,693,420 - +4.3 3,693,451 + +4.4 3,693,898 + BWI76_RS17755 0.88 +1.9 3,694,273 + BWI76_RS17760 0.57 -2.1 3,694,333 - BWI76_RS17760 0.74 -1.2 3,694,611 - BWI76_RS17765 0.40 -0.3 3,694,655 - BWI76_RS17765 0.50 +0.2 3,694,948 + -1.3 3,694,977 + -0.4 3,695,186 - BWI76_RS17770 0.32 -0.5 3,695,420 + BWI76_RS17770 0.68 +0.1
Or see this region's nucleotide sequence