Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17760

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17750 and BWI76_RS17755 are separated by 130 nucleotidesBWI76_RS17755 and BWI76_RS17760 are separated by 118 nucleotidesBWI76_RS17760 and BWI76_RS17765 are separated by 8 nucleotidesBWI76_RS17765 and BWI76_RS17770 are separated by 94 nucleotides BWI76_RS17750: BWI76_RS17750 - Na+/H+ antiporter NhaB, at 3,691,745 to 3,693,298 _RS17750 BWI76_RS17755: BWI76_RS17755 - disulfide bond formation protein B, at 3,693,429 to 3,693,959 _RS17755 BWI76_RS17760: BWI76_RS17760 - hypothetical protein, at 3,694,078 to 3,694,422 _RS17760 BWI76_RS17765: BWI76_RS17765 - hypothetical protein, at 3,694,431 to 3,694,877 _RS17765 BWI76_RS17770: BWI76_RS17770 - isomerase/hydrolase, at 3,694,972 to 3,695,631 _RS17770 Position (kb) 3694 3695Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3693.189 kb on + strandat 3693.397 kb on + strandat 3693.420 kb on - strandat 3693.451 kb on + strandat 3693.898 kb on + strand, within BWI76_RS17755at 3694.273 kb on + strand, within BWI76_RS17760at 3694.333 kb on - strand, within BWI76_RS17760at 3694.611 kb on - strand, within BWI76_RS17765at 3694.655 kb on - strand, within BWI76_RS17765at 3694.948 kb on + strandat 3694.977 kb on + strandat 3695.186 kb on - strand, within BWI76_RS17770at 3695.420 kb on + strand, within BWI76_RS17770

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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3,693,189 + +1.4
3,693,397 + -0.7
3,693,420 - +4.3
3,693,451 + +4.4
3,693,898 + BWI76_RS17755 0.88 +1.9
3,694,273 + BWI76_RS17760 0.57 -2.1
3,694,333 - BWI76_RS17760 0.74 -1.2
3,694,611 - BWI76_RS17765 0.40 -0.3
3,694,655 - BWI76_RS17765 0.50 +0.2
3,694,948 + -1.3
3,694,977 + -0.4
3,695,186 - BWI76_RS17770 0.32 -0.5
3,695,420 + BWI76_RS17770 0.68 +0.1

Or see this region's nucleotide sequence