Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17515 and BWI76_RS17520 are separated by 12 nucleotides BWI76_RS17520 and BWI76_RS17525 are separated by 48 nucleotides BWI76_RS17525 and BWI76_RS17530 overlap by 96 nucleotides
BWI76_RS17515: BWI76_RS17515 - dihydropyrimidinase, at 3,632,423 to 3,633,829
_RS17515
BWI76_RS17520: BWI76_RS17520 - carbamate kinase, at 3,633,842 to 3,634,774
_RS17520
BWI76_RS17525: BWI76_RS17525 - XshC-Cox1-family protein, at 3,634,823 to 3,635,656
_RS17525
BWI76_RS17530: BWI76_RS17530 - hypothetical protein, at 3,635,561 to 3,635,938
_RS17530
Position (kb)
3633
3634
3635 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3632.876 kb on - strand, within BWI76_RS17515 at 3633.047 kb on - strand, within BWI76_RS17515 at 3633.173 kb on - strand at 3633.284 kb on + strand, within BWI76_RS17515 at 3633.354 kb on + strand, within BWI76_RS17515 at 3633.904 kb on + strand at 3633.904 kb on + strand at 3634.180 kb on - strand, within BWI76_RS17520 at 3634.396 kb on - strand, within BWI76_RS17520 at 3634.433 kb on + strand, within BWI76_RS17520 at 3634.472 kb on + strand, within BWI76_RS17520 at 3634.782 kb on - strand at 3635.433 kb on - strand, within BWI76_RS17525 at 3635.549 kb on + strand, within BWI76_RS17525 at 3635.563 kb on + strand at 3635.571 kb on - strand at 3635.582 kb on + strand at 3635.590 kb on - strand at 3635.603 kb on + strand at 3635.613 kb on - strand at 3635.615 kb on + strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.631 kb on - strand at 3635.670 kb on - strand, within BWI76_RS17530 at 3635.671 kb on - strand, within BWI76_RS17530 at 3635.685 kb on + strand, within BWI76_RS17530 at 3635.765 kb on + strand, within BWI76_RS17530
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 3,632,876 - BWI76_RS17515 0.32 +0.7 3,633,047 - BWI76_RS17515 0.44 +3.1 3,633,173 - +2.1 3,633,284 + BWI76_RS17515 0.61 -0.7 3,633,354 + BWI76_RS17515 0.66 +0.9 3,633,904 + -0.3 3,633,904 + -1.2 3,634,180 - BWI76_RS17520 0.36 -0.3 3,634,396 - BWI76_RS17520 0.59 -0.2 3,634,433 + BWI76_RS17520 0.63 -0.9 3,634,472 + BWI76_RS17520 0.68 -0.7 3,634,782 - -0.1 3,635,433 - BWI76_RS17525 0.73 -0.8 3,635,549 + BWI76_RS17525 0.87 -1.5 3,635,563 + +0.4 3,635,571 - +1.7 3,635,582 + -0.4 3,635,590 - +0.5 3,635,603 + -0.8 3,635,613 - -0.6 3,635,615 + +1.2 3,635,623 - -1.5 3,635,623 - +0.7 3,635,623 - +0.7 3,635,623 - +0.0 3,635,631 - +0.4 3,635,670 - BWI76_RS17530 0.29 -1.1 3,635,671 - BWI76_RS17530 0.29 +1.2 3,635,685 + BWI76_RS17530 0.33 +1.5 3,635,765 + BWI76_RS17530 0.54 +0.9
Or see this region's nucleotide sequence