Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16945 and BWI76_RS16950 are separated by 126 nucleotides BWI76_RS16950 and BWI76_RS16955 are separated by 26 nucleotides BWI76_RS16955 and BWI76_RS16960 are separated by 509 nucleotides
BWI76_RS16945: BWI76_RS16945 - 4-hydroxyphenylpyruvate dioxygenase, at 3,514,195 to 3,516,048
_RS16945
BWI76_RS16950: BWI76_RS16950 - TetR family transcriptional regulator, at 3,516,175 to 3,516,813
_RS16950
BWI76_RS16955: BWI76_RS16955 - hypothetical protein, at 3,516,840 to 3,517,052
_RS16955
BWI76_RS16960: BWI76_RS16960 - pyruvate kinase, at 3,517,562 to 3,518,974
_RS16960
Position (kb) 3516
3517 Strain fitness (log2 ratio) -2
-1
0
1 at 3515.508 kb on + strand, within BWI76_RS16945 at 3515.633 kb on + strand, within BWI76_RS16945 at 3515.641 kb on - strand, within BWI76_RS16945 at 3515.670 kb on - strand, within BWI76_RS16945 at 3516.302 kb on + strand, within BWI76_RS16950 at 3516.427 kb on + strand, within BWI76_RS16950 at 3516.469 kb on + strand, within BWI76_RS16950 at 3516.965 kb on + strand, within BWI76_RS16955 at 3517.113 kb on + strand at 3517.121 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 3,515,508 + BWI76_RS16945 0.71 -0.7 3,515,633 + BWI76_RS16945 0.78 -2.0 3,515,641 - BWI76_RS16945 0.78 -0.9 3,515,670 - BWI76_RS16945 0.80 +0.1 3,516,302 + BWI76_RS16950 0.20 -0.9 3,516,427 + BWI76_RS16950 0.39 -1.9 3,516,469 + BWI76_RS16950 0.46 -0.5 3,516,965 + BWI76_RS16955 0.59 -2.4 3,517,113 + -0.7 3,517,121 - +1.5
Or see this region's nucleotide sequence