Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16835 and BWI76_RS16840 are separated by 195 nucleotides BWI76_RS16840 and BWI76_RS16845 overlap by 7 nucleotides BWI76_RS16845 and BWI76_RS16850 overlap by 20 nucleotides
BWI76_RS16835: BWI76_RS16835 - TonB-dependent siderophore receptor, at 3,490,225 to 3,492,378
_RS16835
BWI76_RS16840: BWI76_RS16840 - ABC transporter, at 3,492,574 to 3,493,362
_RS16840
BWI76_RS16845: BWI76_RS16845 - ABC transporter permease, at 3,493,356 to 3,494,351
_RS16845
BWI76_RS16850: BWI76_RS16850 - ABC transporter periplasmic substrate-binding protein, at 3,494,332 to 3,495,369
_RS16850
Position (kb)
3492
3493
3494 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3491.591 kb on + strand, within BWI76_RS16835 at 3491.597 kb on - strand, within BWI76_RS16835 at 3491.789 kb on + strand, within BWI76_RS16835 at 3491.808 kb on + strand, within BWI76_RS16835 at 3491.847 kb on + strand, within BWI76_RS16835 at 3491.871 kb on + strand, within BWI76_RS16835 at 3492.086 kb on + strand, within BWI76_RS16835 at 3492.127 kb on - strand, within BWI76_RS16835 at 3492.297 kb on - strand at 3492.470 kb on + strand at 3492.531 kb on + strand at 3492.735 kb on - strand, within BWI76_RS16840 at 3492.765 kb on + strand, within BWI76_RS16840 at 3493.165 kb on - strand, within BWI76_RS16840 at 3493.403 kb on + strand at 3493.432 kb on - strand at 3493.441 kb on + strand at 3493.480 kb on + strand, within BWI76_RS16845 at 3493.493 kb on - strand, within BWI76_RS16845 at 3493.651 kb on - strand, within BWI76_RS16845 at 3493.715 kb on - strand, within BWI76_RS16845 at 3493.903 kb on - strand, within BWI76_RS16845 at 3493.919 kb on + strand, within BWI76_RS16845 at 3494.251 kb on + strand, within BWI76_RS16845
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 3,491,591 + BWI76_RS16835 0.63 +2.5 3,491,597 - BWI76_RS16835 0.64 +0.1 3,491,789 + BWI76_RS16835 0.73 -0.1 3,491,808 + BWI76_RS16835 0.73 -0.1 3,491,847 + BWI76_RS16835 0.75 -0.5 3,491,871 + BWI76_RS16835 0.76 -0.1 3,492,086 + BWI76_RS16835 0.86 +0.3 3,492,127 - BWI76_RS16835 0.88 +0.1 3,492,297 - +1.9 3,492,470 + -1.2 3,492,531 + -0.2 3,492,735 - BWI76_RS16840 0.20 -1.3 3,492,765 + BWI76_RS16840 0.24 +0.1 3,493,165 - BWI76_RS16840 0.75 +3.2 3,493,403 + +1.6 3,493,432 - -0.9 3,493,441 + -3.1 3,493,480 + BWI76_RS16845 0.12 -1.1 3,493,493 - BWI76_RS16845 0.14 +0.7 3,493,651 - BWI76_RS16845 0.30 +0.2 3,493,715 - BWI76_RS16845 0.36 -0.7 3,493,903 - BWI76_RS16845 0.55 +0.3 3,493,919 + BWI76_RS16845 0.57 -0.3 3,494,251 + BWI76_RS16845 0.90 -0.5
Or see this region's nucleotide sequence