Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16795 and BWI76_RS16800 overlap by 14 nucleotides BWI76_RS16800 and BWI76_RS16805 are separated by 65 nucleotides BWI76_RS16805 and BWI76_RS16810 are separated by 95 nucleotides BWI76_RS16810 and BWI76_RS16815 overlap by 4 nucleotides
BWI76_RS16795: BWI76_RS16795 - amino acid ABC transporter ATPase, at 3,482,646 to 3,483,368
_RS16795
BWI76_RS16800: BWI76_RS16800 - amino acid ABC transporter permease, at 3,483,355 to 3,484,119
_RS16800
BWI76_RS16805: BWI76_RS16805 - basic amino acid ABC transporter substrate-binding protein, at 3,484,185 to 3,484,952
_RS16805
BWI76_RS16810: BWI76_RS16810 - RpiR family transcriptional regulator, at 3,485,048 to 3,485,947
_RS16810
BWI76_RS16815: BWI76_RS16815 - periplasmic binding protein, at 3,485,944 to 3,486,891
_RS16815
Position (kb)
3484
3485 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3483.505 kb on - strand, within BWI76_RS16800 at 3484.146 kb on + strand at 3484.161 kb on + strand at 3484.721 kb on - strand, within BWI76_RS16805 at 3484.881 kb on + strand at 3484.949 kb on - strand at 3485.168 kb on + strand, within BWI76_RS16810 at 3485.322 kb on - strand, within BWI76_RS16810 at 3485.639 kb on - strand, within BWI76_RS16810 at 3485.694 kb on - strand, within BWI76_RS16810
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 3,483,505 - BWI76_RS16800 0.20 -0.4 3,484,146 + +0.7 3,484,161 + -0.8 3,484,721 - BWI76_RS16805 0.70 +3.2 3,484,881 + -1.1 3,484,949 - +0.3 3,485,168 + BWI76_RS16810 0.13 +1.2 3,485,322 - BWI76_RS16810 0.30 -0.5 3,485,639 - BWI76_RS16810 0.66 -0.8 3,485,694 - BWI76_RS16810 0.72 +1.3
Or see this region's nucleotide sequence