Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16390 and BWI76_RS16395 are separated by 108 nucleotides BWI76_RS16395 and BWI76_RS16400 are separated by 47 nucleotides BWI76_RS16400 and BWI76_RS16405 are separated by 61 nucleotides
BWI76_RS16390: BWI76_RS16390 - dipeptide/tripeptide permease A, at 3,400,434 to 3,401,939
_RS16390
BWI76_RS16395: BWI76_RS16395 - glutathione S-transferase, at 3,402,048 to 3,402,653
_RS16395
BWI76_RS16400: BWI76_RS16400 - pyridoxal kinase, at 3,402,701 to 3,403,561
_RS16400
BWI76_RS16405: BWI76_RS16405 - tyrosine--tRNA ligase, at 3,403,623 to 3,404,897
_RS16405
Position (kb) 3402
3403 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3401.195 kb on + strand, within BWI76_RS16390 at 3401.301 kb on + strand, within BWI76_RS16390 at 3401.391 kb on - strand, within BWI76_RS16390 at 3402.033 kb on + strand at 3402.111 kb on + strand, within BWI76_RS16395 at 3402.119 kb on - strand, within BWI76_RS16395 at 3402.407 kb on + strand, within BWI76_RS16395 at 3402.565 kb on - strand, within BWI76_RS16395 at 3402.727 kb on + strand at 3403.460 kb on - strand, within BWI76_RS16400 at 3403.528 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 3,401,195 + BWI76_RS16390 0.51 -0.1 3,401,301 + BWI76_RS16390 0.58 -1.1 3,401,391 - BWI76_RS16390 0.64 -0.2 3,402,033 + +0.2 3,402,111 + BWI76_RS16395 0.10 -1.2 3,402,119 - BWI76_RS16395 0.12 -2.6 3,402,407 + BWI76_RS16395 0.59 -1.9 3,402,565 - BWI76_RS16395 0.85 +0.4 3,402,727 + +1.8 3,403,460 - BWI76_RS16400 0.88 -0.2 3,403,528 - -1.4
Or see this region's nucleotide sequence