Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14605 and BWI76_RS14610 are separated by 3 nucleotides BWI76_RS14610 and BWI76_RS14615 are separated by 13 nucleotides BWI76_RS14615 and BWI76_RS14620 overlap by 1 nucleotides
BWI76_RS14605: BWI76_RS14605 - ABC transporter permease, at 3,065,451 to 3,066,461
_RS14605
BWI76_RS14610: BWI76_RS14610 - putative hydrolase, at 3,066,465 to 3,067,544
_RS14610
BWI76_RS14615: BWI76_RS14615 - hypothetical protein, at 3,067,558 to 3,068,313
_RS14615
BWI76_RS14620: BWI76_RS14620 - acetylornithine deacetylase or succinyl-diaminopimelate desuccinylase, at 3,068,313 to 3,069,425
_RS14620
Position (kb)
3066
3067
3068 Strain fitness (log2 ratio)
-1
0
1 at 3065.539 kb on - strand at 3065.720 kb on + strand, within BWI76_RS14605 at 3065.923 kb on - strand, within BWI76_RS14605 at 3066.091 kb on - strand, within BWI76_RS14605 at 3066.231 kb on - strand, within BWI76_RS14605 at 3066.305 kb on + strand, within BWI76_RS14605 at 3066.399 kb on - strand at 3066.900 kb on + strand, within BWI76_RS14610 at 3067.185 kb on - strand, within BWI76_RS14610 at 3067.315 kb on - strand, within BWI76_RS14610 at 3067.504 kb on - strand at 3067.587 kb on + strand at 3067.587 kb on + strand at 3067.595 kb on - strand at 3067.796 kb on + strand, within BWI76_RS14615
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 3,065,539 - -0.1 3,065,720 + BWI76_RS14605 0.27 +1.0 3,065,923 - BWI76_RS14605 0.47 -0.9 3,066,091 - BWI76_RS14605 0.63 -1.6 3,066,231 - BWI76_RS14605 0.77 -0.3 3,066,305 + BWI76_RS14605 0.84 +0.9 3,066,399 - +0.5 3,066,900 + BWI76_RS14610 0.40 -1.0 3,067,185 - BWI76_RS14610 0.67 -0.6 3,067,315 - BWI76_RS14610 0.79 -1.3 3,067,504 - -0.5 3,067,587 + +0.1 3,067,587 + -0.6 3,067,595 - -0.1 3,067,796 + BWI76_RS14615 0.31 -1.6
Or see this region's nucleotide sequence