Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12510

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12500 and BWI76_RS12505 overlap by 10 nucleotidesBWI76_RS12505 and BWI76_RS12510 are separated by 188 nucleotidesBWI76_RS12510 and BWI76_RS12515 are separated by 92 nucleotidesBWI76_RS12515 and BWI76_RS12520 are separated by 257 nucleotides BWI76_RS12500: BWI76_RS12500 - dipeptide epimerase, at 2,610,729 to 2,611,694 _RS12500 BWI76_RS12505: BWI76_RS12505 - murein peptide amidase A, at 2,611,685 to 2,612,398 _RS12505 BWI76_RS12510: BWI76_RS12510 - peptide ABC transporter substrate-binding protein, at 2,612,587 to 2,614,203 _RS12510 BWI76_RS12515: BWI76_RS12515 - protease, at 2,614,296 to 2,614,859 _RS12515 BWI76_RS12520: BWI76_RS12520 - hypothetical protein, at 2,615,117 to 2,615,320 _RS12520 Position (kb) 2612 2613 2614 2615Strain fitness (log2 ratio) -1 0 1 2at 2611.695 kb on - strandat 2612.394 kb on + strandat 2612.401 kb on - strandat 2612.484 kb on + strandat 2612.484 kb on + strandat 2612.592 kb on - strandat 2612.717 kb on - strandat 2612.873 kb on + strand, within BWI76_RS12510at 2612.873 kb on + strand, within BWI76_RS12510at 2612.873 kb on + strand, within BWI76_RS12510at 2612.873 kb on + strand, within BWI76_RS12510at 2612.881 kb on - strand, within BWI76_RS12510at 2613.077 kb on + strand, within BWI76_RS12510at 2613.281 kb on + strand, within BWI76_RS12510at 2613.317 kb on + strand, within BWI76_RS12510at 2613.320 kb on - strand, within BWI76_RS12510at 2613.438 kb on + strand, within BWI76_RS12510at 2613.438 kb on + strand, within BWI76_RS12510at 2613.438 kb on + strand, within BWI76_RS12510at 2613.446 kb on - strand, within BWI76_RS12510at 2613.446 kb on - strand, within BWI76_RS12510at 2613.518 kb on + strand, within BWI76_RS12510at 2613.749 kb on + strand, within BWI76_RS12510at 2613.941 kb on + strand, within BWI76_RS12510at 2613.949 kb on - strand, within BWI76_RS12510at 2614.102 kb on - strandat 2614.168 kb on + strandat 2614.625 kb on - strand, within BWI76_RS12515at 2614.653 kb on + strand, within BWI76_RS12515at 2614.889 kb on + strandat 2614.901 kb on - strandat 2614.901 kb on - strandat 2614.963 kb on + strandat 2615.139 kb on + strand, within BWI76_RS12520

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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2,611,695 - -1.4
2,612,394 + -1.3
2,612,401 - -0.1
2,612,484 + -0.4
2,612,484 + -0.2
2,612,592 - +2.1
2,612,717 - +1.2
2,612,873 + BWI76_RS12510 0.18 +0.5
2,612,873 + BWI76_RS12510 0.18 +1.4
2,612,873 + BWI76_RS12510 0.18 +2.4
2,612,873 + BWI76_RS12510 0.18 +1.0
2,612,881 - BWI76_RS12510 0.18 +1.1
2,613,077 + BWI76_RS12510 0.30 +1.1
2,613,281 + BWI76_RS12510 0.43 +0.8
2,613,317 + BWI76_RS12510 0.45 +1.6
2,613,320 - BWI76_RS12510 0.45 +0.6
2,613,438 + BWI76_RS12510 0.53 +1.0
2,613,438 + BWI76_RS12510 0.53 +2.2
2,613,438 + BWI76_RS12510 0.53 +2.1
2,613,446 - BWI76_RS12510 0.53 +1.8
2,613,446 - BWI76_RS12510 0.53 +1.3
2,613,518 + BWI76_RS12510 0.58 +1.6
2,613,749 + BWI76_RS12510 0.72 +2.5
2,613,941 + BWI76_RS12510 0.84 +2.6
2,613,949 - BWI76_RS12510 0.84 +0.7
2,614,102 - +1.5
2,614,168 + -0.1
2,614,625 - BWI76_RS12515 0.58 +0.2
2,614,653 + BWI76_RS12515 0.63 -0.3
2,614,889 + -0.5
2,614,901 - +0.0
2,614,901 - +2.2
2,614,963 + +2.1
2,615,139 + BWI76_RS12520 0.11 -0.3

Or see this region's nucleotide sequence