Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12500 and BWI76_RS12505 overlap by 10 nucleotides BWI76_RS12505 and BWI76_RS12510 are separated by 188 nucleotides BWI76_RS12510 and BWI76_RS12515 are separated by 92 nucleotides BWI76_RS12515 and BWI76_RS12520 are separated by 257 nucleotides
BWI76_RS12500: BWI76_RS12500 - dipeptide epimerase, at 2,610,729 to 2,611,694
_RS12500
BWI76_RS12505: BWI76_RS12505 - murein peptide amidase A, at 2,611,685 to 2,612,398
_RS12505
BWI76_RS12510: BWI76_RS12510 - peptide ABC transporter substrate-binding protein, at 2,612,587 to 2,614,203
_RS12510
BWI76_RS12515: BWI76_RS12515 - protease, at 2,614,296 to 2,614,859
_RS12515
BWI76_RS12520: BWI76_RS12520 - hypothetical protein, at 2,615,117 to 2,615,320
_RS12520
Position (kb)
2612
2613
2614
2615 Strain fitness (log2 ratio)
-1
0
1
2 at 2611.695 kb on - strand at 2612.394 kb on + strand at 2612.401 kb on - strand at 2612.484 kb on + strand at 2612.484 kb on + strand at 2612.592 kb on - strand at 2612.717 kb on - strand at 2612.873 kb on + strand, within BWI76_RS12510 at 2612.873 kb on + strand, within BWI76_RS12510 at 2612.873 kb on + strand, within BWI76_RS12510 at 2612.873 kb on + strand, within BWI76_RS12510 at 2612.881 kb on - strand, within BWI76_RS12510 at 2613.077 kb on + strand, within BWI76_RS12510 at 2613.281 kb on + strand, within BWI76_RS12510 at 2613.317 kb on + strand, within BWI76_RS12510 at 2613.320 kb on - strand, within BWI76_RS12510 at 2613.438 kb on + strand, within BWI76_RS12510 at 2613.438 kb on + strand, within BWI76_RS12510 at 2613.438 kb on + strand, within BWI76_RS12510 at 2613.446 kb on - strand, within BWI76_RS12510 at 2613.446 kb on - strand, within BWI76_RS12510 at 2613.518 kb on + strand, within BWI76_RS12510 at 2613.749 kb on + strand, within BWI76_RS12510 at 2613.941 kb on + strand, within BWI76_RS12510 at 2613.949 kb on - strand, within BWI76_RS12510 at 2614.102 kb on - strand at 2614.168 kb on + strand at 2614.625 kb on - strand, within BWI76_RS12515 at 2614.653 kb on + strand, within BWI76_RS12515 at 2614.889 kb on + strand at 2614.901 kb on - strand at 2614.901 kb on - strand at 2614.963 kb on + strand at 2615.139 kb on + strand, within BWI76_RS12520
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 2,611,695 - -1.4 2,612,394 + -1.3 2,612,401 - -0.1 2,612,484 + -0.4 2,612,484 + -0.2 2,612,592 - +2.1 2,612,717 - +1.2 2,612,873 + BWI76_RS12510 0.18 +0.5 2,612,873 + BWI76_RS12510 0.18 +1.4 2,612,873 + BWI76_RS12510 0.18 +2.4 2,612,873 + BWI76_RS12510 0.18 +1.0 2,612,881 - BWI76_RS12510 0.18 +1.1 2,613,077 + BWI76_RS12510 0.30 +1.1 2,613,281 + BWI76_RS12510 0.43 +0.8 2,613,317 + BWI76_RS12510 0.45 +1.6 2,613,320 - BWI76_RS12510 0.45 +0.6 2,613,438 + BWI76_RS12510 0.53 +1.0 2,613,438 + BWI76_RS12510 0.53 +2.2 2,613,438 + BWI76_RS12510 0.53 +2.1 2,613,446 - BWI76_RS12510 0.53 +1.8 2,613,446 - BWI76_RS12510 0.53 +1.3 2,613,518 + BWI76_RS12510 0.58 +1.6 2,613,749 + BWI76_RS12510 0.72 +2.5 2,613,941 + BWI76_RS12510 0.84 +2.6 2,613,949 - BWI76_RS12510 0.84 +0.7 2,614,102 - +1.5 2,614,168 + -0.1 2,614,625 - BWI76_RS12515 0.58 +0.2 2,614,653 + BWI76_RS12515 0.63 -0.3 2,614,889 + -0.5 2,614,901 - +0.0 2,614,901 - +2.2 2,614,963 + +2.1 2,615,139 + BWI76_RS12520 0.11 -0.3
Or see this region's nucleotide sequence