Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11940 and BWI76_RS11945 are separated by 56 nucleotides BWI76_RS11945 and BWI76_RS11950 are separated by 161 nucleotides BWI76_RS11950 and BWI76_RS11955 are separated by 135 nucleotides BWI76_RS11955 and BWI76_RS11960 are separated by 6 nucleotides
BWI76_RS11940: BWI76_RS11940 - aconitate hydratase 1, at 2,513,339 to 2,516,011
_RS11940
BWI76_RS11945: BWI76_RS11945 - GTP cyclohydrolase II, at 2,516,068 to 2,516,658
_RS11945
BWI76_RS11950: BWI76_RS11950 - phosphatidylglycerophosphatase B, at 2,516,820 to 2,517,587
_RS11950
BWI76_RS11955: BWI76_RS11955 - hypothetical protein, at 2,517,723 to 2,518,031
_RS11955
BWI76_RS11960: BWI76_RS11960 - lipopolysaccharide assembly protein LapB, at 2,518,038 to 2,519,207
_RS11960
Position (kb)
2516
2517
2518 Strain fitness (log2 ratio)
-1
0
1 at 2516.688 kb on - strand at 2516.753 kb on - strand at 2516.942 kb on + strand, within BWI76_RS11950 at 2516.947 kb on - strand, within BWI76_RS11950 at 2517.084 kb on + strand, within BWI76_RS11950 at 2517.098 kb on - strand, within BWI76_RS11950 at 2517.339 kb on + strand, within BWI76_RS11950 at 2517.340 kb on + strand, within BWI76_RS11950 at 2517.345 kb on + strand, within BWI76_RS11950 at 2517.523 kb on - strand at 2517.610 kb on - strand at 2517.649 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 2,516,688 - +0.9 2,516,753 - -0.9 2,516,942 + BWI76_RS11950 0.16 +0.3 2,516,947 - BWI76_RS11950 0.17 -0.4 2,517,084 + BWI76_RS11950 0.34 -1.4 2,517,098 - BWI76_RS11950 0.36 -0.5 2,517,339 + BWI76_RS11950 0.68 +0.4 2,517,340 + BWI76_RS11950 0.68 -1.6 2,517,345 + BWI76_RS11950 0.68 -0.2 2,517,523 - +0.6 2,517,610 - -0.3 2,517,649 - +0.4
Or see this region's nucleotide sequence