Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11240 and BWI76_RS11245 overlap by 4 nucleotides BWI76_RS11245 and BWI76_RS11250 overlap by 4 nucleotides BWI76_RS11250 and BWI76_RS11255 overlap by 17 nucleotides
BWI76_RS11240: BWI76_RS11240 - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 2,371,207 to 2,372,253
_RS11240
BWI76_RS11245: BWI76_RS11245 - spermidine/putrescine ABC transporter permease PotC, at 2,372,250 to 2,373,035
_RS11245
BWI76_RS11250: BWI76_RS11250 - spermidine/putrescine ABC transporter permease PotB, at 2,373,032 to 2,373,889
_RS11250
BWI76_RS11255: BWI76_RS11255 - putrescine/spermidine ABC transporter ATP-binding protein, at 2,373,873 to 2,375,009
_RS11255
Position (kb)
2372
2373
2374 Strain fitness (log2 ratio)
-1
0
1 at 2371.571 kb on + strand, within BWI76_RS11240 at 2371.715 kb on + strand, within BWI76_RS11240 at 2371.725 kb on + strand, within BWI76_RS11240 at 2371.762 kb on - strand, within BWI76_RS11240 at 2371.867 kb on - strand, within BWI76_RS11240 at 2372.461 kb on - strand, within BWI76_RS11245 at 2372.599 kb on - strand, within BWI76_RS11245 at 2372.922 kb on + strand, within BWI76_RS11245 at 2373.411 kb on + strand, within BWI76_RS11250 at 2373.732 kb on - strand, within BWI76_RS11250 at 2373.837 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 2,371,571 + BWI76_RS11240 0.35 +1.2 2,371,715 + BWI76_RS11240 0.49 +1.1 2,371,725 + BWI76_RS11240 0.49 +1.3 2,371,762 - BWI76_RS11240 0.53 +1.7 2,371,867 - BWI76_RS11240 0.63 +1.3 2,372,461 - BWI76_RS11245 0.27 +0.7 2,372,599 - BWI76_RS11245 0.44 +1.0 2,372,922 + BWI76_RS11245 0.85 +0.8 2,373,411 + BWI76_RS11250 0.44 -0.2 2,373,732 - BWI76_RS11250 0.82 +0.7 2,373,837 - +0.4
Or see this region's nucleotide sequence