Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10965

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10960 and BWI76_RS10965 are separated by 209 nucleotidesBWI76_RS10965 and BWI76_RS10970 overlap by 15 nucleotidesBWI76_RS10970 and BWI76_RS10975 are separated by 73 nucleotides BWI76_RS10960: BWI76_RS10960 - lipid A biosynthesis lauroyl acyltransferase, at 2,313,862 to 2,314,782 _RS10960 BWI76_RS10965: BWI76_RS10965 - sulfurtransferase, at 2,314,992 to 2,316,056 _RS10965 BWI76_RS10970: BWI76_RS10970 - DUF2770 domain-containing protein, at 2,316,042 to 2,316,155 _RS10970 BWI76_RS10975: BWI76_RS10975 - N-methyltryptophan oxidase, at 2,316,229 to 2,317,347 _RS10975 Position (kb) 2314 2315 2316 2317Strain fitness (log2 ratio) -2 -1 0 1 2at 2314.790 kb on - strandat 2314.790 kb on - strandat 2314.790 kb on - strandat 2314.931 kb on - strandat 2314.984 kb on + strandat 2314.992 kb on - strandat 2315.034 kb on - strandat 2315.050 kb on + strandat 2315.050 kb on + strandat 2315.058 kb on - strandat 2315.121 kb on + strandat 2315.138 kb on + strand, within BWI76_RS10965at 2315.151 kb on + strand, within BWI76_RS10965at 2315.205 kb on + strand, within BWI76_RS10965at 2315.306 kb on + strand, within BWI76_RS10965at 2315.320 kb on + strand, within BWI76_RS10965at 2315.909 kb on - strand, within BWI76_RS10965at 2316.539 kb on - strand, within BWI76_RS10975at 2316.610 kb on - strand, within BWI76_RS10975

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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2,314,790 - -0.6
2,314,790 - -0.9
2,314,790 - +2.3
2,314,931 - -0.3
2,314,984 + -1.5
2,314,992 - -0.9
2,315,034 - -0.3
2,315,050 + +0.3
2,315,050 + -1.3
2,315,058 - +2.1
2,315,121 + +1.2
2,315,138 + BWI76_RS10965 0.14 -1.1
2,315,151 + BWI76_RS10965 0.15 +0.0
2,315,205 + BWI76_RS10965 0.20 -0.1
2,315,306 + BWI76_RS10965 0.29 -1.0
2,315,320 + BWI76_RS10965 0.31 +0.3
2,315,909 - BWI76_RS10965 0.86 -2.1
2,316,539 - BWI76_RS10975 0.28 +1.5
2,316,610 - BWI76_RS10975 0.34 +0.9

Or see this region's nucleotide sequence