Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08010

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08005 and BWI76_RS08010 are separated by 137 nucleotidesBWI76_RS08010 and BWI76_RS08015 are separated by 91 nucleotidesBWI76_RS08015 and BWI76_RS08020 are separated by 106 nucleotides BWI76_RS08005: BWI76_RS08005 - transcriptional regulator, at 1,697,375 to 1,698,328 _RS08005 BWI76_RS08010: BWI76_RS08010 - octanoyltransferase, at 1,698,466 to 1,699,110 _RS08010 BWI76_RS08015: BWI76_RS08015 - hypothetical protein, at 1,699,202 to 1,699,465 _RS08015 BWI76_RS08020: BWI76_RS08020 - serine-type D-Ala-D-Ala carboxypeptidase, at 1,699,572 to 1,700,771 _RS08020 Position (kb) 1698 1699 1700Strain fitness (log2 ratio) -2 -1 0 1at 1697.575 kb on - strand, within BWI76_RS08005at 1697.970 kb on + strand, within BWI76_RS08005at 1698.083 kb on + strand, within BWI76_RS08005at 1698.091 kb on - strand, within BWI76_RS08005at 1698.140 kb on + strand, within BWI76_RS08005at 1698.211 kb on - strand, within BWI76_RS08005at 1698.357 kb on + strandat 1698.357 kb on - strandat 1698.365 kb on - strandat 1698.459 kb on - strandat 1699.151 kb on + strandat 1699.755 kb on - strand, within BWI76_RS08020

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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1,697,575 - BWI76_RS08005 0.21 +0.9
1,697,970 + BWI76_RS08005 0.62 +0.4
1,698,083 + BWI76_RS08005 0.74 -1.2
1,698,091 - BWI76_RS08005 0.75 -0.8
1,698,140 + BWI76_RS08005 0.80 -0.6
1,698,211 - BWI76_RS08005 0.88 -0.8
1,698,357 + -2.2
1,698,357 - -2.0
1,698,365 - -2.5
1,698,459 - -1.5
1,699,151 + -0.9
1,699,755 - BWI76_RS08020 0.15 +1.1

Or see this region's nucleotide sequence