Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07575 and BWI76_RS07580 are separated by 49 nucleotides BWI76_RS07580 and BWI76_RS07585 are separated by 14 nucleotides BWI76_RS07585 and BWI76_RS07590 overlap by 8 nucleotides
BWI76_RS07575: BWI76_RS07575 - ABC transporter substrate-binding protein, at 1,604,685 to 1,605,536
_RS07575
BWI76_RS07580: BWI76_RS07580 - polar amino acid ABC transporter inner membrane subunit, at 1,605,586 to 1,606,227
_RS07580
BWI76_RS07585: BWI76_RS07585 - polar amino acid ABC transporter inner membrane subunit, at 1,606,242 to 1,606,907
_RS07585
BWI76_RS07590: BWI76_RS07590 - glutamate ABC transporter ATP-binding protein, at 1,606,900 to 1,607,661
_RS07590
Position (kb)
1606
1607 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1605.293 kb on + strand, within BWI76_RS07575 at 1605.299 kb on + strand, within BWI76_RS07575 at 1605.312 kb on - strand, within BWI76_RS07575 at 1605.332 kb on - strand, within BWI76_RS07575 at 1605.391 kb on - strand, within BWI76_RS07575 at 1605.510 kb on - strand at 1605.510 kb on - strand at 1605.510 kb on - strand at 1605.510 kb on - strand at 1605.536 kb on - strand at 1605.693 kb on + strand, within BWI76_RS07580 at 1605.726 kb on + strand, within BWI76_RS07580 at 1605.754 kb on - strand, within BWI76_RS07580 at 1605.919 kb on - strand, within BWI76_RS07580 at 1606.014 kb on + strand, within BWI76_RS07580 at 1606.048 kb on - strand, within BWI76_RS07580 at 1606.053 kb on - strand, within BWI76_RS07580 at 1606.112 kb on + strand, within BWI76_RS07580 at 1606.120 kb on - strand, within BWI76_RS07580 at 1606.173 kb on + strand at 1606.183 kb on + strand at 1606.188 kb on + strand at 1606.203 kb on - strand at 1606.203 kb on - strand at 1606.231 kb on - strand at 1606.234 kb on - strand at 1606.262 kb on - strand at 1606.269 kb on + strand at 1606.303 kb on + strand at 1606.311 kb on - strand at 1606.311 kb on - strand, within BWI76_RS07585 at 1606.361 kb on - strand, within BWI76_RS07585 at 1606.489 kb on + strand, within BWI76_RS07585 at 1606.489 kb on + strand, within BWI76_RS07585 at 1606.496 kb on + strand, within BWI76_RS07585 at 1606.504 kb on - strand, within BWI76_RS07585 at 1606.504 kb on - strand, within BWI76_RS07585 at 1606.505 kb on - strand, within BWI76_RS07585 at 1606.527 kb on - strand, within BWI76_RS07585 at 1606.532 kb on - strand, within BWI76_RS07585 at 1606.562 kb on - strand, within BWI76_RS07585 at 1606.596 kb on - strand, within BWI76_RS07585 at 1606.596 kb on - strand at 1606.616 kb on - strand, within BWI76_RS07585 at 1606.666 kb on + strand, within BWI76_RS07585 at 1606.705 kb on + strand, within BWI76_RS07585 at 1606.740 kb on + strand, within BWI76_RS07585 at 1606.748 kb on - strand, within BWI76_RS07585 at 1606.753 kb on - strand, within BWI76_RS07585 at 1606.756 kb on - strand at 1606.760 kb on + strand, within BWI76_RS07585 at 1606.760 kb on + strand, within BWI76_RS07585 at 1606.768 kb on - strand, within BWI76_RS07585 at 1606.768 kb on - strand, within BWI76_RS07585 at 1606.818 kb on + strand, within BWI76_RS07585 at 1606.910 kb on - strand at 1607.111 kb on + strand, within BWI76_RS07590 at 1607.365 kb on - strand, within BWI76_RS07590 at 1607.437 kb on - strand, within BWI76_RS07590 at 1607.671 kb on - strand at 1607.697 kb on + strand at 1607.788 kb on - strand at 1607.788 kb on - strand at 1607.813 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 1,605,293 + BWI76_RS07575 0.71 -1.6 1,605,299 + BWI76_RS07575 0.72 +0.3 1,605,312 - BWI76_RS07575 0.74 -0.8 1,605,332 - BWI76_RS07575 0.76 +1.1 1,605,391 - BWI76_RS07575 0.83 -0.1 1,605,510 - -1.0 1,605,510 - -0.2 1,605,510 - +0.8 1,605,510 - -0.2 1,605,536 - +0.9 1,605,693 + BWI76_RS07580 0.17 +0.8 1,605,726 + BWI76_RS07580 0.22 +1.6 1,605,754 - BWI76_RS07580 0.26 +1.2 1,605,919 - BWI76_RS07580 0.52 -0.0 1,606,014 + BWI76_RS07580 0.67 +2.3 1,606,048 - BWI76_RS07580 0.72 -1.6 1,606,053 - BWI76_RS07580 0.73 -1.3 1,606,112 + BWI76_RS07580 0.82 -0.1 1,606,120 - BWI76_RS07580 0.83 -0.2 1,606,173 + -1.0 1,606,183 + -1.1 1,606,188 + +1.6 1,606,203 - +3.3 1,606,203 - -0.5 1,606,231 - +0.7 1,606,234 - -0.8 1,606,262 - -0.3 1,606,269 + -0.4 1,606,303 + -0.1 1,606,311 - +2.8 1,606,311 - BWI76_RS07585 0.10 +0.3 1,606,361 - BWI76_RS07585 0.18 +2.5 1,606,489 + BWI76_RS07585 0.37 -1.7 1,606,489 + BWI76_RS07585 0.37 +0.2 1,606,496 + BWI76_RS07585 0.38 -0.1 1,606,504 - BWI76_RS07585 0.39 +3.8 1,606,504 - BWI76_RS07585 0.39 +0.9 1,606,505 - BWI76_RS07585 0.39 +1.5 1,606,527 - BWI76_RS07585 0.43 -0.5 1,606,532 - BWI76_RS07585 0.44 +0.3 1,606,562 - BWI76_RS07585 0.48 +0.1 1,606,596 - BWI76_RS07585 0.53 -0.5 1,606,596 - +1.5 1,606,616 - BWI76_RS07585 0.56 +0.3 1,606,666 + BWI76_RS07585 0.64 -0.1 1,606,705 + BWI76_RS07585 0.70 -1.0 1,606,740 + BWI76_RS07585 0.75 +1.1 1,606,748 - BWI76_RS07585 0.76 +0.9 1,606,753 - BWI76_RS07585 0.77 -0.2 1,606,756 - +1.5 1,606,760 + BWI76_RS07585 0.78 +0.2 1,606,760 + BWI76_RS07585 0.78 +0.8 1,606,768 - BWI76_RS07585 0.79 -0.5 1,606,768 - BWI76_RS07585 0.79 -0.1 1,606,818 + BWI76_RS07585 0.86 -1.5 1,606,910 - -2.2 1,607,111 + BWI76_RS07590 0.28 -0.4 1,607,365 - BWI76_RS07590 0.61 -0.1 1,607,437 - BWI76_RS07590 0.70 -0.4 1,607,671 - -0.2 1,607,697 + +0.2 1,607,788 - -1.0 1,607,788 - -0.5 1,607,813 - +0.8
Or see this region's nucleotide sequence