Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05180 and BWI76_RS05185 are separated by 191 nucleotides BWI76_RS05185 and BWI76_RS05190 are separated by 46 nucleotides BWI76_RS05190 and BWI76_RS05195 are separated by 107 nucleotides
BWI76_RS05180: BWI76_RS05180 - serine endoprotease DegQ, at 1,089,597 to 1,091,036
_RS05180
BWI76_RS05185: BWI76_RS05185 - CdaR family transcriptional regulator, at 1,091,228 to 1,092,385
_RS05185
BWI76_RS05190: BWI76_RS05190 - hypothetical protein, at 1,092,432 to 1,092,821
_RS05190
BWI76_RS05195: BWI76_RS05195 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 1,092,929 to 1,093,753
_RS05195
Position (kb)
1091
1092
1093 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1090.247 kb on - strand, within BWI76_RS05180 at 1090.247 kb on - strand, within BWI76_RS05180 at 1090.331 kb on + strand, within BWI76_RS05180 at 1090.338 kb on - strand, within BWI76_RS05180 at 1090.606 kb on - strand, within BWI76_RS05180 at 1090.800 kb on + strand, within BWI76_RS05180 at 1090.806 kb on - strand, within BWI76_RS05180 at 1090.942 kb on + strand at 1091.147 kb on - strand at 1091.200 kb on + strand at 1091.263 kb on + strand at 1091.271 kb on - strand at 1091.457 kb on + strand, within BWI76_RS05185 at 1091.548 kb on + strand, within BWI76_RS05185 at 1091.616 kb on - strand, within BWI76_RS05185 at 1091.627 kb on + strand, within BWI76_RS05185 at 1091.662 kb on - strand, within BWI76_RS05185 at 1091.803 kb on + strand, within BWI76_RS05185 at 1091.806 kb on - strand, within BWI76_RS05185 at 1091.806 kb on - strand, within BWI76_RS05185 at 1092.204 kb on + strand, within BWI76_RS05185 at 1092.268 kb on + strand, within BWI76_RS05185 at 1092.308 kb on - strand at 1092.344 kb on + strand at 1092.354 kb on + strand at 1092.357 kb on - strand at 1092.357 kb on - strand at 1092.374 kb on - strand at 1092.599 kb on - strand, within BWI76_RS05190 at 1092.599 kb on - strand, within BWI76_RS05190 at 1092.604 kb on - strand, within BWI76_RS05190 at 1092.627 kb on + strand, within BWI76_RS05190 at 1092.627 kb on + strand, within BWI76_RS05190 at 1092.627 kb on + strand, within BWI76_RS05190 at 1092.627 kb on - strand at 1092.635 kb on - strand, within BWI76_RS05190 at 1092.635 kb on - strand, within BWI76_RS05190 at 1092.635 kb on - strand, within BWI76_RS05190 at 1092.706 kb on + strand, within BWI76_RS05190 at 1092.706 kb on + strand, within BWI76_RS05190 at 1092.714 kb on - strand, within BWI76_RS05190 at 1092.755 kb on + strand, within BWI76_RS05190 at 1092.784 kb on + strand at 1092.789 kb on + strand at 1092.794 kb on + strand at 1092.802 kb on - strand at 1092.818 kb on + strand at 1092.837 kb on - strand at 1092.927 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 1,090,247 - BWI76_RS05180 0.45 -0.6 1,090,247 - BWI76_RS05180 0.45 +2.5 1,090,331 + BWI76_RS05180 0.51 +1.1 1,090,338 - BWI76_RS05180 0.51 +0.6 1,090,606 - BWI76_RS05180 0.70 -1.4 1,090,800 + BWI76_RS05180 0.84 +0.3 1,090,806 - BWI76_RS05180 0.84 -1.2 1,090,942 + -0.6 1,091,147 - -0.4 1,091,200 + -0.5 1,091,263 + +0.5 1,091,271 - +0.5 1,091,457 + BWI76_RS05185 0.20 +0.7 1,091,548 + BWI76_RS05185 0.28 -1.4 1,091,616 - BWI76_RS05185 0.34 +0.5 1,091,627 + BWI76_RS05185 0.34 -2.1 1,091,662 - BWI76_RS05185 0.37 -1.2 1,091,803 + BWI76_RS05185 0.50 -1.0 1,091,806 - BWI76_RS05185 0.50 -0.6 1,091,806 - BWI76_RS05185 0.50 +0.9 1,092,204 + BWI76_RS05185 0.84 +0.1 1,092,268 + BWI76_RS05185 0.90 -0.8 1,092,308 - +0.1 1,092,344 + -0.2 1,092,354 + +2.6 1,092,357 - +2.5 1,092,357 - +2.2 1,092,374 - +0.7 1,092,599 - BWI76_RS05190 0.43 +0.3 1,092,599 - BWI76_RS05190 0.43 -1.8 1,092,604 - BWI76_RS05190 0.44 +1.9 1,092,627 + BWI76_RS05190 0.50 -1.5 1,092,627 + BWI76_RS05190 0.50 -0.2 1,092,627 + BWI76_RS05190 0.50 +0.9 1,092,627 - +0.7 1,092,635 - BWI76_RS05190 0.52 -0.3 1,092,635 - BWI76_RS05190 0.52 -0.4 1,092,635 - BWI76_RS05190 0.52 +0.4 1,092,706 + BWI76_RS05190 0.70 +0.4 1,092,706 + BWI76_RS05190 0.70 +1.1 1,092,714 - BWI76_RS05190 0.72 +0.2 1,092,755 + BWI76_RS05190 0.83 -0.9 1,092,784 + +0.5 1,092,789 + +0.5 1,092,794 + +0.7 1,092,802 - -0.3 1,092,818 + +0.2 1,092,837 - -0.5 1,092,927 + +1.6
Or see this region's nucleotide sequence