Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05150 and BWI76_RS05155 are separated by 81 nucleotides BWI76_RS05155 and BWI76_RS05160 are separated by 92 nucleotides BWI76_RS05160 and BWI76_RS05165 are separated by 180 nucleotides BWI76_RS05165 and BWI76_RS05170 overlap by 8 nucleotides
BWI76_RS05150: BWI76_RS05150 - ClC family H(+)/Cl(-) exchange transporter, at 1,083,589 to 1,085,049
_RS05150
BWI76_RS05155: BWI76_RS05155 - iron-sulfur cluster insertion protein ErpA, at 1,085,131 to 1,085,475
_RS05155
BWI76_RS05160: BWI76_RS05160 - hypothetical protein, at 1,085,568 to 1,086,191
_RS05160
BWI76_RS05165: BWI76_RS05165 - cobalamin-binding protein, at 1,086,372 to 1,087,172
_RS05165
BWI76_RS05170: BWI76_RS05170 - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, at 1,087,165 to 1,087,863
_RS05170
Position (kb)
1085
1086
1087 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1084.650 kb on + strand, within BWI76_RS05150 at 1084.655 kb on + strand, within BWI76_RS05150 at 1084.658 kb on - strand, within BWI76_RS05150 at 1084.853 kb on + strand, within BWI76_RS05150 at 1084.853 kb on + strand, within BWI76_RS05150 at 1084.965 kb on - strand at 1085.778 kb on + strand, within BWI76_RS05160 at 1085.855 kb on + strand, within BWI76_RS05160 at 1085.884 kb on + strand, within BWI76_RS05160 at 1086.174 kb on - strand at 1086.197 kb on + strand at 1086.204 kb on + strand at 1086.306 kb on + strand at 1086.314 kb on - strand at 1086.399 kb on + strand at 1086.425 kb on - strand at 1086.445 kb on - strand at 1086.540 kb on + strand, within BWI76_RS05165 at 1086.566 kb on + strand, within BWI76_RS05165 at 1086.574 kb on - strand, within BWI76_RS05165 at 1086.666 kb on + strand, within BWI76_RS05165 at 1087.021 kb on + strand, within BWI76_RS05165 at 1087.094 kb on - strand at 1087.133 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 1,084,650 + BWI76_RS05150 0.73 -0.1 1,084,655 + BWI76_RS05150 0.73 +0.1 1,084,658 - BWI76_RS05150 0.73 -1.9 1,084,853 + BWI76_RS05150 0.87 +0.7 1,084,853 + BWI76_RS05150 0.87 +0.1 1,084,965 - +1.1 1,085,778 + BWI76_RS05160 0.34 -2.4 1,085,855 + BWI76_RS05160 0.46 -0.5 1,085,884 + BWI76_RS05160 0.51 -0.8 1,086,174 - -0.7 1,086,197 + -0.1 1,086,204 + -1.1 1,086,306 + -1.3 1,086,314 - +0.5 1,086,399 + -0.3 1,086,425 - -0.5 1,086,445 - +0.0 1,086,540 + BWI76_RS05165 0.21 -0.8 1,086,566 + BWI76_RS05165 0.24 +3.1 1,086,574 - BWI76_RS05165 0.25 -1.5 1,086,666 + BWI76_RS05165 0.37 +1.7 1,087,021 + BWI76_RS05165 0.81 -0.6 1,087,094 - -1.3 1,087,133 + +1.1
Or see this region's nucleotide sequence