Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04560 and BWI76_RS04565 are separated by 81 nucleotides BWI76_RS04565 and BWI76_RS04570 are separated by 12 nucleotides BWI76_RS04570 and BWI76_RS04575 are separated by 187 nucleotides
BWI76_RS04560: BWI76_RS04560 - holo-ACP synthase CitX, at 945,490 to 946,029
_RS04560
BWI76_RS04565: BWI76_RS04565 - bifunctional tRNA pseudouridine(32) synthase/ribosomal large subunit pseudouridine synthase RluA, at 946,111 to 946,770
_RS04565
BWI76_RS04570: BWI76_RS04570 - RNA polymerase-associated protein RapA, at 946,783 to 949,689
_RS04570
BWI76_RS04575: BWI76_RS04575 - DNA polymerase II, at 949,877 to 952,234
_RS04575
Position (kb) 946
947
948
949
950 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 945.809 kb on - strand, within BWI76_RS04560 at 946.053 kb on - strand at 946.098 kb on - strand at 946.108 kb on - strand at 946.342 kb on + strand, within BWI76_RS04565 at 946.398 kb on + strand, within BWI76_RS04565 at 946.407 kb on + strand, within BWI76_RS04565 at 946.474 kb on - strand, within BWI76_RS04565 at 946.543 kb on + strand, within BWI76_RS04565 at 946.554 kb on + strand, within BWI76_RS04565 at 947.054 kb on + strand at 947.054 kb on + strand at 947.129 kb on - strand, within BWI76_RS04570 at 947.349 kb on + strand, within BWI76_RS04570 at 947.595 kb on - strand at 947.824 kb on + strand, within BWI76_RS04570 at 948.289 kb on - strand, within BWI76_RS04570 at 948.550 kb on - strand, within BWI76_RS04570 at 948.641 kb on + strand, within BWI76_RS04570 at 948.695 kb on - strand, within BWI76_RS04570 at 948.784 kb on - strand, within BWI76_RS04570 at 948.965 kb on - strand, within BWI76_RS04570 at 948.970 kb on + strand, within BWI76_RS04570 at 949.017 kb on + strand, within BWI76_RS04570 at 949.025 kb on - strand, within BWI76_RS04570 at 949.520 kb on + strand at 949.766 kb on - strand at 949.800 kb on - strand at 949.939 kb on - strand at 949.988 kb on - strand at 950.031 kb on + strand at 950.082 kb on + strand at 950.143 kb on + strand, within BWI76_RS04575 at 950.162 kb on + strand, within BWI76_RS04575 at 950.170 kb on - strand, within BWI76_RS04575 at 950.210 kb on - strand, within BWI76_RS04575 at 950.339 kb on - strand, within BWI76_RS04575 at 950.463 kb on + strand, within BWI76_RS04575 at 950.463 kb on - strand, within BWI76_RS04575
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 945,809 - BWI76_RS04560 0.59 +1.0 946,053 - +0.8 946,098 - -0.2 946,108 - -0.1 946,342 + BWI76_RS04565 0.35 -0.2 946,398 + BWI76_RS04565 0.43 -0.6 946,407 + BWI76_RS04565 0.45 +3.5 946,474 - BWI76_RS04565 0.55 +0.3 946,543 + BWI76_RS04565 0.65 +0.4 946,554 + BWI76_RS04565 0.67 -0.6 947,054 + +1.4 947,054 + -0.9 947,129 - BWI76_RS04570 0.12 +1.7 947,349 + BWI76_RS04570 0.19 +1.5 947,595 - +4.5 947,824 + BWI76_RS04570 0.36 +0.6 948,289 - BWI76_RS04570 0.52 +0.8 948,550 - BWI76_RS04570 0.61 -1.5 948,641 + BWI76_RS04570 0.64 +0.9 948,695 - BWI76_RS04570 0.66 -0.8 948,784 - BWI76_RS04570 0.69 +1.1 948,965 - BWI76_RS04570 0.75 +1.1 948,970 + BWI76_RS04570 0.75 +2.1 949,017 + BWI76_RS04570 0.77 +1.0 949,025 - BWI76_RS04570 0.77 +1.3 949,520 + +1.6 949,766 - -0.4 949,800 - -1.1 949,939 - -0.2 949,988 - -0.3 950,031 + -0.9 950,082 + +1.1 950,143 + BWI76_RS04575 0.11 -1.4 950,162 + BWI76_RS04575 0.12 -0.1 950,170 - BWI76_RS04575 0.12 -0.6 950,210 - BWI76_RS04575 0.14 -1.0 950,339 - BWI76_RS04575 0.20 -0.0 950,463 + BWI76_RS04575 0.25 +0.6 950,463 - BWI76_RS04575 0.25 -1.6
Or see this region's nucleotide sequence