Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03010 and BWI76_RS03015 are separated by 164 nucleotides BWI76_RS03015 and BWI76_RS03020 are separated by 52 nucleotides BWI76_RS03020 and BWI76_RS03025 are separated by 99 nucleotides BWI76_RS03025 and BWI76_RS03030 are separated by 123 nucleotides
BWI76_RS03010: BWI76_RS03010 - ornithine carbamoyltransferase, at 615,898 to 616,902
_RS03010
BWI76_RS03015: BWI76_RS03015 - RNase E inhibitor protein, at 617,067 to 617,492
_RS03015
BWI76_RS03020: BWI76_RS03020 - tRNA hydroxylase, at 617,545 to 618,303
_RS03020
BWI76_RS03025: BWI76_RS03025 - N-acetyltransferase, at 618,403 to 618,906
_RS03025
BWI76_RS03030: BWI76_RS03030 - valine--tRNA ligase, at 619,030 to 621,885
_RS03030
Position (kb) 617
618
619 Strain fitness (log2 ratio) -2
-1
0
1
2 at 616.695 kb on + strand, within BWI76_RS03010 at 616.808 kb on + strand at 616.808 kb on + strand at 616.808 kb on + strand at 616.808 kb on + strand at 616.808 kb on + strand at 616.816 kb on - strand at 616.844 kb on - strand at 616.906 kb on - strand at 617.167 kb on - strand, within BWI76_RS03015 at 617.323 kb on + strand, within BWI76_RS03015 at 617.607 kb on - strand at 617.654 kb on - strand, within BWI76_RS03020 at 617.665 kb on - strand, within BWI76_RS03020 at 617.665 kb on - strand, within BWI76_RS03020 at 617.761 kb on - strand, within BWI76_RS03020 at 617.995 kb on - strand, within BWI76_RS03020 at 617.995 kb on - strand, within BWI76_RS03020 at 618.331 kb on - strand at 618.410 kb on + strand at 618.565 kb on + strand, within BWI76_RS03025 at 618.573 kb on - strand, within BWI76_RS03025 at 618.605 kb on - strand, within BWI76_RS03025 at 618.884 kb on - strand at 618.885 kb on - strand at 618.966 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 616,695 + BWI76_RS03010 0.79 +1.0 616,808 + -1.1 616,808 + +0.5 616,808 + -2.2 616,808 + -0.2 616,808 + -0.2 616,816 - +0.3 616,844 - +0.3 616,906 - +0.3 617,167 - BWI76_RS03015 0.23 +0.5 617,323 + BWI76_RS03015 0.60 -1.3 617,607 - +0.5 617,654 - BWI76_RS03020 0.14 +0.2 617,665 - BWI76_RS03020 0.16 -1.0 617,665 - BWI76_RS03020 0.16 +1.2 617,761 - BWI76_RS03020 0.28 +1.3 617,995 - BWI76_RS03020 0.59 +1.5 617,995 - BWI76_RS03020 0.59 +0.8 618,331 - +1.8 618,410 + -0.3 618,565 + BWI76_RS03025 0.32 -0.3 618,573 - BWI76_RS03025 0.34 +1.1 618,605 - BWI76_RS03025 0.40 -1.5 618,884 - +0.3 618,885 - -0.1 618,966 + -0.7
Or see this region's nucleotide sequence