Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02270 and BWI76_RS02275 are separated by 36 nucleotides BWI76_RS02275 and BWI76_RS02280 are separated by 115 nucleotides BWI76_RS02280 and BWI76_RS02285 are separated by 145 nucleotides BWI76_RS02285 and BWI76_RS02290 are separated by 34 nucleotides
BWI76_RS02270: BWI76_RS02270 - DSBA oxidoreductase, at 466,760 to 467,542
_RS02270
BWI76_RS02275: BWI76_RS02275 - TetR family transcriptional regulator, at 467,579 to 468,175
_RS02275
BWI76_RS02280: BWI76_RS02280 - NAD(P)H dehydrogenase (quinone), at 468,291 to 468,878
_RS02280
BWI76_RS02285: BWI76_RS02285 - hypothetical protein, at 469,024 to 469,419
_RS02285
BWI76_RS02290: BWI76_RS02290 - hypothetical protein, at 469,454 to 470,263
_RS02290
Position (kb) 468
469 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 467.605 kb on - strand at 467.639 kb on - strand, within BWI76_RS02275 at 467.668 kb on - strand, within BWI76_RS02275 at 467.693 kb on + strand, within BWI76_RS02275 at 467.725 kb on + strand at 467.938 kb on - strand, within BWI76_RS02275 at 467.963 kb on - strand, within BWI76_RS02275 at 468.144 kb on - strand at 468.151 kb on - strand at 468.320 kb on + strand at 468.351 kb on - strand, within BWI76_RS02280 at 468.483 kb on - strand, within BWI76_RS02280 at 468.504 kb on - strand, within BWI76_RS02280 at 468.592 kb on + strand, within BWI76_RS02280 at 468.616 kb on + strand, within BWI76_RS02280 at 468.661 kb on - strand, within BWI76_RS02280 at 468.714 kb on - strand, within BWI76_RS02280 at 468.729 kb on - strand, within BWI76_RS02280 at 468.770 kb on + strand, within BWI76_RS02280 at 468.798 kb on - strand, within BWI76_RS02280 at 468.806 kb on - strand, within BWI76_RS02280 at 468.808 kb on + strand, within BWI76_RS02280 at 468.811 kb on - strand, within BWI76_RS02280 at 468.831 kb on - strand at 468.845 kb on - strand at 468.867 kb on + strand at 468.867 kb on - strand at 468.887 kb on + strand at 468.916 kb on + strand at 468.916 kb on + strand at 468.917 kb on + strand at 468.917 kb on + strand at 468.917 kb on + strand at 468.917 kb on + strand at 468.924 kb on - strand at 468.924 kb on - strand at 468.926 kb on + strand at 468.926 kb on + strand at 468.934 kb on - strand at 468.986 kb on - strand at 468.986 kb on - strand at 468.988 kb on - strand at 469.029 kb on - strand at 469.035 kb on + strand at 469.053 kb on + strand at 469.059 kb on + strand at 469.059 kb on + strand at 469.059 kb on + strand at 469.059 kb on + strand at 469.059 kb on + strand at 469.059 kb on + strand at 469.061 kb on - strand at 469.067 kb on - strand, within BWI76_RS02285 at 469.067 kb on - strand, within BWI76_RS02285 at 469.067 kb on - strand, within BWI76_RS02285 at 469.067 kb on - strand, within BWI76_RS02285 at 469.067 kb on - strand, within BWI76_RS02285 at 469.067 kb on - strand, within BWI76_RS02285 at 469.067 kb on - strand, within BWI76_RS02285 at 469.067 kb on - strand, within BWI76_RS02285 at 469.069 kb on - strand, within BWI76_RS02285 at 469.244 kb on + strand, within BWI76_RS02285 at 469.275 kb on + strand, within BWI76_RS02285 at 469.277 kb on + strand, within BWI76_RS02285 at 469.277 kb on + strand, within BWI76_RS02285 at 469.277 kb on + strand, within BWI76_RS02285 at 469.277 kb on + strand, within BWI76_RS02285 at 469.277 kb on + strand, within BWI76_RS02285 at 469.353 kb on - strand, within BWI76_RS02285 at 469.372 kb on + strand, within BWI76_RS02285 at 469.372 kb on + strand, within BWI76_RS02285 at 469.372 kb on + strand, within BWI76_RS02285 at 469.372 kb on + strand, within BWI76_RS02285 at 469.372 kb on + strand, within BWI76_RS02285 at 469.372 kb on + strand, within BWI76_RS02285 at 469.372 kb on + strand, within BWI76_RS02285 at 469.372 kb on + strand, within BWI76_RS02285 at 469.408 kb on - strand at 469.411 kb on - strand at 469.533 kb on + strand at 469.542 kb on - strand, within BWI76_RS02290 at 469.542 kb on - strand, within BWI76_RS02290 at 469.542 kb on - strand, within BWI76_RS02290 at 469.599 kb on - strand, within BWI76_RS02290 at 469.608 kb on + strand, within BWI76_RS02290 at 469.608 kb on + strand at 469.608 kb on + strand, within BWI76_RS02290 at 469.608 kb on + strand at 469.616 kb on - strand, within BWI76_RS02290 at 469.616 kb on - strand, within BWI76_RS02290 at 469.616 kb on - strand, within BWI76_RS02290 at 469.650 kb on - strand, within BWI76_RS02290 at 469.739 kb on + strand, within BWI76_RS02290 at 469.739 kb on + strand, within BWI76_RS02290 at 469.783 kb on + strand, within BWI76_RS02290 at 469.791 kb on - strand, within BWI76_RS02290 at 469.791 kb on - strand, within BWI76_RS02290 at 469.795 kb on + strand, within BWI76_RS02290 at 469.803 kb on - strand, within BWI76_RS02290 at 469.819 kb on + strand, within BWI76_RS02290 at 469.834 kb on - strand, within BWI76_RS02290 at 469.841 kb on - strand, within BWI76_RS02290 at 469.841 kb on - strand, within BWI76_RS02290 at 469.843 kb on - strand, within BWI76_RS02290 at 469.855 kb on + strand, within BWI76_RS02290 at 469.855 kb on + strand, within BWI76_RS02290 at 469.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 467,605 - +0.8 467,639 - BWI76_RS02275 0.10 -0.6 467,668 - BWI76_RS02275 0.15 +0.3 467,693 + BWI76_RS02275 0.19 +2.9 467,725 + +4.0 467,938 - BWI76_RS02275 0.60 -1.0 467,963 - BWI76_RS02275 0.64 +0.4 468,144 - -1.4 468,151 - +0.9 468,320 + -0.3 468,351 - BWI76_RS02280 0.10 -1.0 468,483 - BWI76_RS02280 0.33 +0.1 468,504 - BWI76_RS02280 0.36 -1.0 468,592 + BWI76_RS02280 0.51 +0.2 468,616 + BWI76_RS02280 0.55 -1.3 468,661 - BWI76_RS02280 0.63 -0.9 468,714 - BWI76_RS02280 0.72 +0.4 468,729 - BWI76_RS02280 0.74 -0.0 468,770 + BWI76_RS02280 0.81 +0.5 468,798 - BWI76_RS02280 0.86 -1.0 468,806 - BWI76_RS02280 0.88 -0.1 468,808 + BWI76_RS02280 0.88 -1.3 468,811 - BWI76_RS02280 0.88 -1.0 468,831 - +0.7 468,845 - +1.0 468,867 + -0.7 468,867 - -0.3 468,887 + -1.7 468,916 + -2.5 468,916 + +2.2 468,917 + +1.1 468,917 + +1.0 468,917 + +0.6 468,917 + -0.2 468,924 - -0.0 468,924 - +1.4 468,926 + +0.0 468,926 + -1.4 468,934 - +0.7 468,986 - -1.4 468,986 - -0.1 468,988 - +0.1 469,029 - +0.1 469,035 + -1.1 469,053 + +0.5 469,059 + -0.7 469,059 + +0.1 469,059 + -1.9 469,059 + -0.3 469,059 + +0.4 469,059 + -1.0 469,061 - -1.5 469,067 - BWI76_RS02285 0.11 -0.8 469,067 - BWI76_RS02285 0.11 +0.8 469,067 - BWI76_RS02285 0.11 -0.4 469,067 - BWI76_RS02285 0.11 -0.0 469,067 - BWI76_RS02285 0.11 +0.4 469,067 - BWI76_RS02285 0.11 +1.1 469,067 - BWI76_RS02285 0.11 +0.7 469,067 - BWI76_RS02285 0.11 -1.1 469,069 - BWI76_RS02285 0.11 +0.4 469,244 + BWI76_RS02285 0.56 +0.5 469,275 + BWI76_RS02285 0.63 -1.7 469,277 + BWI76_RS02285 0.64 +0.8 469,277 + BWI76_RS02285 0.64 +2.6 469,277 + BWI76_RS02285 0.64 +0.8 469,277 + BWI76_RS02285 0.64 +1.3 469,277 + BWI76_RS02285 0.64 +0.3 469,353 - BWI76_RS02285 0.83 +0.8 469,372 + BWI76_RS02285 0.88 +0.3 469,372 + BWI76_RS02285 0.88 -0.4 469,372 + BWI76_RS02285 0.88 -1.6 469,372 + BWI76_RS02285 0.88 -1.1 469,372 + BWI76_RS02285 0.88 -1.2 469,372 + BWI76_RS02285 0.88 +2.0 469,372 + BWI76_RS02285 0.88 +0.4 469,372 + BWI76_RS02285 0.88 +0.2 469,408 - -1.1 469,411 - -1.2 469,533 + +2.0 469,542 - BWI76_RS02290 0.11 +0.2 469,542 - BWI76_RS02290 0.11 -0.5 469,542 - BWI76_RS02290 0.11 +0.4 469,599 - BWI76_RS02290 0.18 -0.0 469,608 + BWI76_RS02290 0.19 +0.4 469,608 + +2.0 469,608 + BWI76_RS02290 0.19 +0.7 469,608 + +0.6 469,616 - BWI76_RS02290 0.20 -0.7 469,616 - BWI76_RS02290 0.20 +1.1 469,616 - BWI76_RS02290 0.20 -0.4 469,650 - BWI76_RS02290 0.24 -0.3 469,739 + BWI76_RS02290 0.35 -0.1 469,739 + BWI76_RS02290 0.35 -2.8 469,783 + BWI76_RS02290 0.41 +1.0 469,791 - BWI76_RS02290 0.42 +2.8 469,791 - BWI76_RS02290 0.42 +1.8 469,795 + BWI76_RS02290 0.42 -1.6 469,803 - BWI76_RS02290 0.43 +0.6 469,819 + BWI76_RS02290 0.45 +1.6 469,834 - BWI76_RS02290 0.47 -1.4 469,841 - BWI76_RS02290 0.48 -0.1 469,841 - BWI76_RS02290 0.48 +0.6 469,843 - BWI76_RS02290 0.48 +0.1 469,855 + BWI76_RS02290 0.50 +0.1 469,855 + BWI76_RS02290 0.50 -0.3 469,863 - +2.6
Or see this region's nucleotide sequence