Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01175

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01170 and BWI76_RS01175 are separated by 21 nucleotidesBWI76_RS01175 and BWI76_RS01180 overlap by 4 nucleotides BWI76_RS01170: BWI76_RS01170 - uroporphyrinogen-III C-methyltransferase, at 238,826 to 239,995 _RS01170 BWI76_RS01175: BWI76_RS01175 - uroporphyrinogen-III synthase, at 240,017 to 240,757 _RS01175 BWI76_RS01180: BWI76_RS01180 - porphobilinogen deaminase, at 240,754 to 241,695 _RS01180 Position (kb) 240 241Strain fitness (log2 ratio) -1 0 1at 239.119 kb on - strand, within BWI76_RS01170at 239.155 kb on + strand, within BWI76_RS01170at 239.528 kb on + strand, within BWI76_RS01170at 239.545 kb on + strand, within BWI76_RS01170at 239.939 kb on + strandat 241.724 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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239,119 - BWI76_RS01170 0.25 +1.5
239,155 + BWI76_RS01170 0.28 -0.7
239,528 + BWI76_RS01170 0.60 -0.2
239,545 + BWI76_RS01170 0.61 -0.4
239,939 + -0.9
241,724 - -0.7

Or see this region's nucleotide sequence