Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01165 and BWI76_RS01170 are separated by 3 nucleotides BWI76_RS01170 and BWI76_RS01175 are separated by 21 nucleotides BWI76_RS01175 and BWI76_RS01180 overlap by 4 nucleotides
BWI76_RS01165: BWI76_RS01165 - protoheme IX biogenesis protein HemY, at 237,626 to 238,822
_RS01165
BWI76_RS01170: BWI76_RS01170 - uroporphyrinogen-III C-methyltransferase, at 238,826 to 239,995
_RS01170
BWI76_RS01175: BWI76_RS01175 - uroporphyrinogen-III synthase, at 240,017 to 240,757
_RS01175
BWI76_RS01180: BWI76_RS01180 - porphobilinogen deaminase, at 240,754 to 241,695
_RS01180
Position (kb) 238
239
240 Strain fitness (log2 ratio) -2
-1
0
1
2 at 237.830 kb on + strand, within BWI76_RS01165 at 237.855 kb on + strand, within BWI76_RS01165 at 237.889 kb on - strand, within BWI76_RS01165 at 238.154 kb on + strand, within BWI76_RS01165 at 238.162 kb on - strand, within BWI76_RS01165 at 238.230 kb on + strand, within BWI76_RS01165 at 238.564 kb on + strand, within BWI76_RS01165 at 238.944 kb on - strand, within BWI76_RS01170 at 239.119 kb on - strand, within BWI76_RS01170 at 239.155 kb on + strand, within BWI76_RS01170 at 239.528 kb on + strand, within BWI76_RS01170 at 239.545 kb on + strand, within BWI76_RS01170 at 239.939 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 237,830 + BWI76_RS01165 0.17 +0.6 237,855 + BWI76_RS01165 0.19 +0.6 237,889 - BWI76_RS01165 0.22 +0.2 238,154 + BWI76_RS01165 0.44 +0.3 238,162 - BWI76_RS01165 0.45 -1.4 238,230 + BWI76_RS01165 0.50 -1.0 238,564 + BWI76_RS01165 0.78 -2.4 238,944 - BWI76_RS01170 0.10 +2.3 239,119 - BWI76_RS01170 0.25 +1.5 239,155 + BWI76_RS01170 0.28 -0.7 239,528 + BWI76_RS01170 0.60 -0.2 239,545 + BWI76_RS01170 0.61 -0.4 239,939 + -0.9
Or see this region's nucleotide sequence