Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00450 and BWI76_RS00455 are separated by 343 nucleotides BWI76_RS00455 and BWI76_RS00460 overlap by 4 nucleotides BWI76_RS00460 and BWI76_RS00465 are separated by 12 nucleotides
BWI76_RS00450: BWI76_RS00450 - formate dehydrogenase accessory protein FdhE, at 91,941 to 92,870
_RS00450
BWI76_RS00455: BWI76_RS00455 - formate dehydrogenase cytochrome b556 subunit, at 93,214 to 93,849
_RS00455
BWI76_RS00460: BWI76_RS00460 - formate dehydrogenase subunit beta, at 93,846 to 94,748
_RS00460
BWI76_RS00465: BWI76_RS00465 - formate dehydrogenase-N subunit alpha, at 94,761 to 97,175
_RS00465
Position (kb) 93
94
95 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 92.851 kb on - strand at 93.001 kb on - strand at 93.015 kb on + strand at 93.037 kb on + strand at 93.052 kb on - strand at 93.067 kb on + strand at 93.079 kb on + strand at 93.123 kb on + strand at 93.135 kb on - strand at 94.744 kb on + strand at 94.789 kb on + strand at 94.789 kb on + strand at 95.082 kb on - strand, within BWI76_RS00465 at 95.176 kb on - strand, within BWI76_RS00465 at 95.176 kb on - strand, within BWI76_RS00465 at 95.501 kb on + strand, within BWI76_RS00465 at 95.501 kb on + strand, within BWI76_RS00465 at 95.503 kb on - strand, within BWI76_RS00465 at 95.509 kb on - strand, within BWI76_RS00465 at 95.518 kb on + strand, within BWI76_RS00465
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 92,851 - +1.1 93,001 - +0.2 93,015 + +0.3 93,037 + -1.9 93,052 - +0.5 93,067 + +1.0 93,079 + -2.0 93,123 + +0.1 93,135 - +1.1 94,744 + +0.4 94,789 + +3.4 94,789 + +0.6 95,082 - BWI76_RS00465 0.13 +1.9 95,176 - BWI76_RS00465 0.17 +1.1 95,176 - BWI76_RS00465 0.17 +1.6 95,501 + BWI76_RS00465 0.31 +1.8 95,501 + BWI76_RS00465 0.31 -0.3 95,503 - BWI76_RS00465 0.31 +1.8 95,509 - BWI76_RS00465 0.31 +1.5 95,518 + BWI76_RS00465 0.31 +1.7
Or see this region's nucleotide sequence