Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00450 and BWI76_RS00455 are separated by 343 nucleotides BWI76_RS00455 and BWI76_RS00460 overlap by 4 nucleotides BWI76_RS00460 and BWI76_RS00465 are separated by 12 nucleotides
BWI76_RS00450: BWI76_RS00450 - formate dehydrogenase accessory protein FdhE, at 91,941 to 92,870
_RS00450
BWI76_RS00455: BWI76_RS00455 - formate dehydrogenase cytochrome b556 subunit, at 93,214 to 93,849
_RS00455
BWI76_RS00460: BWI76_RS00460 - formate dehydrogenase subunit beta, at 93,846 to 94,748
_RS00460
BWI76_RS00465: BWI76_RS00465 - formate dehydrogenase-N subunit alpha, at 94,761 to 97,175
_RS00465
Position (kb) 93
94 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 92.222 kb on + strand, within BWI76_RS00450 at 92.226 kb on + strand, within BWI76_RS00450 at 92.251 kb on - strand, within BWI76_RS00450 at 92.555 kb on + strand, within BWI76_RS00450 at 92.601 kb on + strand, within BWI76_RS00450 at 92.610 kb on - strand, within BWI76_RS00450 at 92.682 kb on - strand, within BWI76_RS00450 at 92.851 kb on - strand at 93.001 kb on - strand at 93.015 kb on + strand at 93.037 kb on + strand at 93.052 kb on - strand at 93.067 kb on + strand at 93.079 kb on + strand at 93.123 kb on + strand at 93.135 kb on - strand at 94.744 kb on + strand at 94.789 kb on + strand at 94.789 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 92,222 + BWI76_RS00450 0.30 +1.8 92,226 + BWI76_RS00450 0.31 -0.5 92,251 - BWI76_RS00450 0.33 +2.4 92,555 + BWI76_RS00450 0.66 +0.1 92,601 + BWI76_RS00450 0.71 +1.4 92,610 - BWI76_RS00450 0.72 +0.4 92,682 - BWI76_RS00450 0.80 +3.0 92,851 - +1.1 93,001 - +0.2 93,015 + +0.3 93,037 + -1.9 93,052 - +0.5 93,067 + +1.0 93,079 + -2.0 93,123 + +0.1 93,135 - +1.1 94,744 + +0.4 94,789 + +3.4 94,789 + +0.6
Or see this region's nucleotide sequence