Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27945

Experiment: nitrite 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27935 and BWI76_RS27940 overlap by 4 nucleotidesBWI76_RS27940 and BWI76_RS27945 overlap by 17 nucleotidesBWI76_RS27945 and BWI76_RS27950 overlap by 4 nucleotidesBWI76_RS27950 and BWI76_RS27955 are separated by 243 nucleotides BWI76_RS27935: BWI76_RS27935 - galactonate dehydratase, at 5,793,557 to 5,794,705 _RS27935 BWI76_RS27940: BWI76_RS27940 - 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, at 5,794,702 to 5,795,319 _RS27940 BWI76_RS27945: BWI76_RS27945 - 2-oxo-3-deoxygalactonate kinase, at 5,795,303 to 5,796,178 _RS27945 BWI76_RS27950: BWI76_RS27950 - transcriptional regulator, at 5,796,175 to 5,796,864 _RS27950 BWI76_RS27955: BWI76_RS27955 - sugar-phosphatase, at 5,797,108 to 5,797,920 _RS27955 Position (kb) 5795 5796 5797Strain fitness (log2 ratio) -2 -1 0 1at 5794.360 kb on - strand, within BWI76_RS27935at 5794.487 kb on + strand, within BWI76_RS27935at 5794.509 kb on + strand, within BWI76_RS27935at 5794.529 kb on - strand, within BWI76_RS27935at 5794.573 kb on - strand, within BWI76_RS27935at 5794.587 kb on - strand, within BWI76_RS27935at 5794.715 kb on - strandat 5794.914 kb on - strandat 5795.026 kb on - strand, within BWI76_RS27940at 5795.105 kb on - strand, within BWI76_RS27940at 5795.111 kb on + strand, within BWI76_RS27940at 5795.276 kb on - strandat 5795.291 kb on - strandat 5795.330 kb on + strandat 5795.338 kb on - strandat 5795.409 kb on - strand, within BWI76_RS27945at 5795.707 kb on - strand, within BWI76_RS27945at 5795.707 kb on - strand, within BWI76_RS27945at 5795.815 kb on + strand, within BWI76_RS27945at 5796.152 kb on + strandat 5796.328 kb on - strand, within BWI76_RS27950at 5796.356 kb on - strand, within BWI76_RS27950at 5796.381 kb on + strand, within BWI76_RS27950at 5796.791 kb on - strand, within BWI76_RS27950at 5796.828 kb on + strandat 5796.860 kb on + strandat 5796.891 kb on - strandat 5797.154 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 20 mM
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5,794,360 - BWI76_RS27935 0.70 -0.5
5,794,487 + BWI76_RS27935 0.81 +0.5
5,794,509 + BWI76_RS27935 0.83 +0.9
5,794,529 - BWI76_RS27935 0.85 -0.8
5,794,573 - BWI76_RS27935 0.88 -0.3
5,794,587 - BWI76_RS27935 0.90 +0.6
5,794,715 - +0.6
5,794,914 - +0.1
5,795,026 - BWI76_RS27940 0.52 +1.0
5,795,105 - BWI76_RS27940 0.65 -0.4
5,795,111 + BWI76_RS27940 0.66 +0.7
5,795,276 - -0.2
5,795,291 - +0.8
5,795,330 + -0.4
5,795,338 - +1.5
5,795,409 - BWI76_RS27945 0.12 -0.8
5,795,707 - BWI76_RS27945 0.46 -1.2
5,795,707 - BWI76_RS27945 0.46 -1.7
5,795,815 + BWI76_RS27945 0.58 -0.4
5,796,152 + -0.3
5,796,328 - BWI76_RS27950 0.22 +1.3
5,796,356 - BWI76_RS27950 0.26 +0.1
5,796,381 + BWI76_RS27950 0.30 +1.2
5,796,791 - BWI76_RS27950 0.89 +0.7
5,796,828 + +1.2
5,796,860 + +0.0
5,796,891 - -2.3
5,797,154 + -0.4

Or see this region's nucleotide sequence