Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20950

Experiment: nitrite 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20940 and BWI76_RS20945 are separated by 95 nucleotidesBWI76_RS20945 and BWI76_RS20950 overlap by 63 nucleotidesBWI76_RS20950 and BWI76_RS20955 are separated by 54 nucleotides BWI76_RS20940: BWI76_RS20940 - uracil/xanthine transporter, at 4,377,577 to 4,378,866 _RS20940 BWI76_RS20945: BWI76_RS20945 - uracil phosphoribosyltransferase, at 4,378,962 to 4,379,588 _RS20945 BWI76_RS20950: BWI76_RS20950 - hypothetical protein, at 4,379,526 to 4,379,716 _RS20950 BWI76_RS20955: BWI76_RS20955 - 6-phospho-beta-glucosidase, at 4,379,771 to 4,381,201 _RS20955 Position (kb) 4379 4380Strain fitness (log2 ratio) -1 0 1at 4378.730 kb on + strand, within BWI76_RS20940at 4378.758 kb on - strandat 4379.050 kb on + strand, within BWI76_RS20945at 4379.060 kb on - strand, within BWI76_RS20945at 4379.261 kb on + strand, within BWI76_RS20945at 4379.477 kb on - strand, within BWI76_RS20945at 4379.701 kb on + strandat 4379.701 kb on + strandat 4379.709 kb on - strandat 4379.943 kb on - strand, within BWI76_RS20955at 4380.026 kb on - strand, within BWI76_RS20955at 4380.122 kb on + strand, within BWI76_RS20955at 4380.301 kb on - strand, within BWI76_RS20955at 4380.302 kb on - strand, within BWI76_RS20955at 4380.324 kb on + strand, within BWI76_RS20955at 4380.359 kb on + strand, within BWI76_RS20955at 4380.359 kb on + strand, within BWI76_RS20955at 4380.367 kb on - strand, within BWI76_RS20955at 4380.522 kb on - strand, within BWI76_RS20955

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 20 mM
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4,378,730 + BWI76_RS20940 0.89 -1.2
4,378,758 - +1.3
4,379,050 + BWI76_RS20945 0.14 -0.7
4,379,060 - BWI76_RS20945 0.16 -0.3
4,379,261 + BWI76_RS20945 0.48 -1.6
4,379,477 - BWI76_RS20945 0.82 -0.2
4,379,701 + +0.7
4,379,701 + +0.2
4,379,709 - -0.1
4,379,943 - BWI76_RS20955 0.12 +0.7
4,380,026 - BWI76_RS20955 0.18 +0.7
4,380,122 + BWI76_RS20955 0.25 -0.1
4,380,301 - BWI76_RS20955 0.37 +0.6
4,380,302 - BWI76_RS20955 0.37 +0.6
4,380,324 + BWI76_RS20955 0.39 +1.0
4,380,359 + BWI76_RS20955 0.41 -0.3
4,380,359 + BWI76_RS20955 0.41 -0.8
4,380,367 - BWI76_RS20955 0.42 +0.6
4,380,522 - BWI76_RS20955 0.52 -0.9

Or see this region's nucleotide sequence