Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20935

Experiment: nitrite 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20925 and BWI76_RS20930 are separated by 9 nucleotidesBWI76_RS20930 and BWI76_RS20935 are separated by 28 nucleotidesBWI76_RS20935 and BWI76_RS20940 are separated by 71 nucleotides BWI76_RS20925: BWI76_RS20925 - peptidase M48, at 4,374,919 to 4,376,382 _RS20925 BWI76_RS20930: BWI76_RS20930 - arsenate reductase (glutaredoxin), at 4,376,392 to 4,376,751 _RS20930 BWI76_RS20935: BWI76_RS20935 - DnaA regulatory inactivator Hda, at 4,376,780 to 4,377,505 _RS20935 BWI76_RS20940: BWI76_RS20940 - uracil/xanthine transporter, at 4,377,577 to 4,378,866 _RS20940 Position (kb) 4376 4377 4378Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4375.849 kb on + strand, within BWI76_RS20925at 4375.860 kb on - strand, within BWI76_RS20925at 4375.877 kb on - strand, within BWI76_RS20925at 4376.118 kb on + strand, within BWI76_RS20925at 4376.308 kb on - strandat 4376.437 kb on - strand, within BWI76_RS20930at 4376.447 kb on + strand, within BWI76_RS20930at 4376.761 kb on + strandat 4377.517 kb on + strandat 4377.548 kb on - strandat 4377.568 kb on + strandat 4377.576 kb on - strandat 4377.703 kb on - strandat 4377.768 kb on - strand, within BWI76_RS20940at 4378.272 kb on + strand, within BWI76_RS20940

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 20 mM
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4,375,849 + BWI76_RS20925 0.64 +3.1
4,375,860 - BWI76_RS20925 0.64 -1.1
4,375,877 - BWI76_RS20925 0.65 +1.9
4,376,118 + BWI76_RS20925 0.82 -0.8
4,376,308 - -1.0
4,376,437 - BWI76_RS20930 0.12 +0.7
4,376,447 + BWI76_RS20930 0.15 +0.8
4,376,761 + -1.2
4,377,517 + -1.6
4,377,548 - -0.8
4,377,568 + -3.3
4,377,576 - -0.0
4,377,703 - +0.3
4,377,768 - BWI76_RS20940 0.15 -0.9
4,378,272 + BWI76_RS20940 0.54 -0.5

Or see this region's nucleotide sequence