Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13875

Experiment: nitrite 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13870 and BWI76_RS13875 are separated by 189 nucleotidesBWI76_RS13875 and BWI76_RS13880 are separated by 44 nucleotidesBWI76_RS13880 and BWI76_RS13885 are separated by 85 nucleotides BWI76_RS13870: BWI76_RS13870 - hypothetical protein, at 2,892,522 to 2,893,439 _RS13870 BWI76_RS13875: BWI76_RS13875 - CbbBc protein, at 2,893,629 to 2,895,929 _RS13875 BWI76_RS13880: BWI76_RS13880 - lysine transporter LysE, at 2,895,974 to 2,896,591 _RS13880 BWI76_RS13885: BWI76_RS13885 - AraC family transcriptional regulator, at 2,896,677 to 2,897,489 _RS13885 Position (kb) 2893 2894 2895 2896Strain fitness (log2 ratio) -2 -1 0 1at 2892.643 kb on + strand, within BWI76_RS13870at 2892.667 kb on + strand, within BWI76_RS13870at 2892.734 kb on + strand, within BWI76_RS13870at 2892.913 kb on + strand, within BWI76_RS13870at 2893.156 kb on + strand, within BWI76_RS13870at 2893.192 kb on - strand, within BWI76_RS13870at 2893.354 kb on - strandat 2893.496 kb on + strandat 2893.505 kb on + strandat 2893.584 kb on + strandat 2893.867 kb on + strand, within BWI76_RS13875at 2894.211 kb on - strand, within BWI76_RS13875at 2894.583 kb on + strand, within BWI76_RS13875at 2895.073 kb on + strand, within BWI76_RS13875at 2895.274 kb on + strand, within BWI76_RS13875at 2895.397 kb on + strand, within BWI76_RS13875at 2895.561 kb on + strand, within BWI76_RS13875at 2895.792 kb on - strandat 2896.045 kb on + strand, within BWI76_RS13880at 2896.468 kb on - strand, within BWI76_RS13880at 2896.549 kb on - strandat 2896.575 kb on + strandat 2896.628 kb on - strandat 2896.632 kb on + strandat 2896.708 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 20 mM
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2,892,643 + BWI76_RS13870 0.13 +0.1
2,892,667 + BWI76_RS13870 0.16 +0.2
2,892,734 + BWI76_RS13870 0.23 -0.7
2,892,913 + BWI76_RS13870 0.43 -0.2
2,893,156 + BWI76_RS13870 0.69 -1.9
2,893,192 - BWI76_RS13870 0.73 -0.3
2,893,354 - -1.9
2,893,496 + -0.3
2,893,505 + +0.7
2,893,584 + +0.0
2,893,867 + BWI76_RS13875 0.10 -1.5
2,894,211 - BWI76_RS13875 0.25 -0.6
2,894,583 + BWI76_RS13875 0.41 -0.9
2,895,073 + BWI76_RS13875 0.63 -1.1
2,895,274 + BWI76_RS13875 0.71 -0.2
2,895,397 + BWI76_RS13875 0.77 +0.4
2,895,561 + BWI76_RS13875 0.84 -0.1
2,895,792 - -0.4
2,896,045 + BWI76_RS13880 0.11 -1.6
2,896,468 - BWI76_RS13880 0.80 +0.2
2,896,549 - -0.4
2,896,575 + +0.5
2,896,628 - -0.9
2,896,632 + -0.3
2,896,708 - +0.1

Or see this region's nucleotide sequence