Experiment: nitrite 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12490 and BWI76_RS12495 are separated by 41 nucleotides BWI76_RS12495 and BWI76_RS12500 are separated by 101 nucleotides BWI76_RS12500 and BWI76_RS12505 overlap by 10 nucleotides BWI76_RS12505 and BWI76_RS12510 are separated by 188 nucleotides
BWI76_RS12490: BWI76_RS12490 - AbrB family transcriptional regulator, at 2,609,021 to 2,610,076
_RS12490
BWI76_RS12495: BWI76_RS12495 - 2-Cys peroxiredoxin, at 2,610,118 to 2,610,627
_RS12495
BWI76_RS12500: BWI76_RS12500 - dipeptide epimerase, at 2,610,729 to 2,611,694
_RS12500
BWI76_RS12505: BWI76_RS12505 - murein peptide amidase A, at 2,611,685 to 2,612,398
_RS12505
BWI76_RS12510: BWI76_RS12510 - peptide ABC transporter substrate-binding protein, at 2,612,587 to 2,614,203
_RS12510
Position (kb)
2610
2611
2612 Strain fitness (log2 ratio)
-1
0
1 at 2609.745 kb on - strand, within BWI76_RS12490 at 2609.988 kb on - strand at 2610.154 kb on + strand at 2610.527 kb on + strand, within BWI76_RS12495 at 2611.041 kb on - strand, within BWI76_RS12500 at 2611.055 kb on + strand, within BWI76_RS12500 at 2611.079 kb on + strand, within BWI76_RS12500 at 2611.497 kb on + strand, within BWI76_RS12500 at 2611.695 kb on - strand at 2612.394 kb on + strand at 2612.401 kb on - strand at 2612.484 kb on + strand at 2612.484 kb on + strand at 2612.592 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 20 mM remove 2,609,745 - BWI76_RS12490 0.69 -0.7 2,609,988 - -1.3 2,610,154 + +0.1 2,610,527 + BWI76_RS12495 0.80 -1.0 2,611,041 - BWI76_RS12500 0.32 -0.9 2,611,055 + BWI76_RS12500 0.34 -0.6 2,611,079 + BWI76_RS12500 0.36 +0.6 2,611,497 + BWI76_RS12500 0.80 -1.7 2,611,695 - -0.1 2,612,394 + -0.2 2,612,401 - +0.7 2,612,484 + -0.4 2,612,484 + +0.9 2,612,592 - -1.7
Or see this region's nucleotide sequence