Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10560

Experiment: nitrite 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10550 and BWI76_RS10555 overlap by 4 nucleotidesBWI76_RS10555 and BWI76_RS10560 are separated by 9 nucleotidesBWI76_RS10560 and BWI76_RS10565 are separated by 168 nucleotides BWI76_RS10550: BWI76_RS10550 - AtzE family amidohydrolase, at 2,228,472 to 2,229,869 _RS10550 BWI76_RS10555: BWI76_RS10555 - hypothetical protein, at 2,229,866 to 2,230,054 _RS10555 BWI76_RS10560: BWI76_RS10560 - gamma-glutamyltransferase, at 2,230,064 to 2,231,647 _RS10560 BWI76_RS10565: BWI76_RS10565 - sugar isomerase, at 2,231,816 to 2,232,655 _RS10565 Position (kb) 2230 2231 2232Strain fitness (log2 ratio) -3 -2 -1 0 1at 2229.082 kb on + strand, within BWI76_RS10550at 2229.087 kb on + strand, within BWI76_RS10550at 2229.203 kb on + strand, within BWI76_RS10550at 2230.028 kb on - strand, within BWI76_RS10555at 2230.180 kb on + strandat 2230.188 kb on - strandat 2230.907 kb on - strand, within BWI76_RS10560at 2230.999 kb on + strand, within BWI76_RS10560at 2231.062 kb on + strand, within BWI76_RS10560at 2231.079 kb on + strand, within BWI76_RS10560at 2231.594 kb on + strandat 2231.926 kb on - strand, within BWI76_RS10565at 2232.085 kb on - strand, within BWI76_RS10565at 2232.153 kb on + strand, within BWI76_RS10565at 2232.155 kb on - strand, within BWI76_RS10565at 2232.228 kb on - strand, within BWI76_RS10565at 2232.320 kb on + strand, within BWI76_RS10565

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 20 mM
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2,229,082 + BWI76_RS10550 0.44 +0.0
2,229,087 + BWI76_RS10550 0.44 +0.5
2,229,203 + BWI76_RS10550 0.52 -0.3
2,230,028 - BWI76_RS10555 0.86 -0.8
2,230,180 + -0.2
2,230,188 - +1.0
2,230,907 - BWI76_RS10560 0.53 -3.3
2,230,999 + BWI76_RS10560 0.59 -1.6
2,231,062 + BWI76_RS10560 0.63 -0.3
2,231,079 + BWI76_RS10560 0.64 -1.0
2,231,594 + -0.7
2,231,926 - BWI76_RS10565 0.13 -0.6
2,232,085 - BWI76_RS10565 0.32 -0.2
2,232,153 + BWI76_RS10565 0.40 -0.1
2,232,155 - BWI76_RS10565 0.40 +0.9
2,232,228 - BWI76_RS10565 0.49 -0.4
2,232,320 + BWI76_RS10565 0.60 +1.0

Or see this region's nucleotide sequence