Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09455

Experiment: nitrite 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09450 and BWI76_RS09455 are separated by 89 nucleotidesBWI76_RS09455 and BWI76_RS09460 are separated by 349 nucleotides BWI76_RS09450: BWI76_RS09450 - 30S ribosomal protein S6--L-glutamate ligase, at 1,978,755 to 1,979,657 _RS09450 BWI76_RS09455: BWI76_RS09455 - YbjN domain-containing protein, at 1,979,747 to 1,980,226 _RS09455 BWI76_RS09460: BWI76_RS09460 - spermidine/putrescine ABC transporter substrate-binding protein PotF, at 1,980,576 to 1,981,688 _RS09460 Position (kb) 1979 1980 1981Strain fitness (log2 ratio) -1 0 1 2 3at 1978.787 kb on + strandat 1979.000 kb on + strand, within BWI76_RS09450at 1979.534 kb on + strand, within BWI76_RS09450at 1979.932 kb on + strand, within BWI76_RS09455at 1980.089 kb on - strand, within BWI76_RS09455at 1980.110 kb on - strand, within BWI76_RS09455at 1980.153 kb on - strand, within BWI76_RS09455at 1980.174 kb on - strand, within BWI76_RS09455at 1980.403 kb on - strandat 1980.419 kb on + strandat 1980.606 kb on + strandat 1980.611 kb on + strandat 1980.611 kb on + strandat 1980.619 kb on - strandat 1980.619 kb on - strandat 1980.675 kb on - strandat 1980.790 kb on - strand, within BWI76_RS09460at 1980.816 kb on - strand, within BWI76_RS09460at 1980.864 kb on - strand, within BWI76_RS09460at 1981.028 kb on + strand, within BWI76_RS09460at 1981.036 kb on - strand, within BWI76_RS09460

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 20 mM
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1,978,787 + -0.1
1,979,000 + BWI76_RS09450 0.27 +0.2
1,979,534 + BWI76_RS09450 0.86 +0.8
1,979,932 + BWI76_RS09455 0.39 -0.1
1,980,089 - BWI76_RS09455 0.71 -0.2
1,980,110 - BWI76_RS09455 0.76 +2.1
1,980,153 - BWI76_RS09455 0.85 +3.6
1,980,174 - BWI76_RS09455 0.89 +1.9
1,980,403 - -1.0
1,980,419 + -0.1
1,980,606 + -0.2
1,980,611 + -1.2
1,980,611 + -0.3
1,980,619 - +0.6
1,980,619 - -0.0
1,980,675 - -0.3
1,980,790 - BWI76_RS09460 0.19 +1.3
1,980,816 - BWI76_RS09460 0.22 +0.3
1,980,864 - BWI76_RS09460 0.26 +0.1
1,981,028 + BWI76_RS09460 0.41 -0.1
1,981,036 - BWI76_RS09460 0.41 +1.6

Or see this region's nucleotide sequence