Experiment: Citric Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1361 and Psyr_1362 are separated by 14 nucleotides Psyr_1362 and Psyr_1363 are separated by 138 nucleotides Psyr_1363 and Psyr_1364 are separated by 112 nucleotides Psyr_1364 and Psyr_1365 are separated by 13 nucleotides
Psyr_1361: Psyr_1361 - CTP synthase, at 1,544,354 to 1,545,985
_1361
Psyr_1362: Psyr_1362 - 2-dehydro-3-deoxyphosphooctonate aldolase, at 1,546,000 to 1,546,845
_1362
Psyr_1363: Psyr_1363 - enolase, at 1,546,984 to 1,548,270
_1363
Psyr_1364: Psyr_1364 - cell division protein FtsB, at 1,548,383 to 1,548,739
_1364
Psyr_1365: Psyr_1365 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 1,548,753 to 1,549,463
_1365
Position (kb)
1546
1547
1548
1549 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1547.193 kb on + strand, within Psyr_1363 at 1547.413 kb on + strand, within Psyr_1363 at 1548.193 kb on + strand at 1548.241 kb on + strand at 1548.241 kb on + strand at 1548.241 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.276 kb on - strand at 1548.276 kb on - strand at 1548.276 kb on - strand at 1548.276 kb on - strand at 1548.382 kb on + strand at 1548.382 kb on + strand at 1548.382 kb on + strand at 1548.382 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Citric Acid (C) remove 1,547,193 + Psyr_1363 0.16 -1.0 1,547,413 + Psyr_1363 0.33 -1.0 1,548,193 + -2.6 1,548,241 + -2.0 1,548,241 + -2.0 1,548,241 + -3.6 1,548,275 + -1.3 1,548,275 + -1.6 1,548,275 + -3.4 1,548,275 + -1.9 1,548,275 + -0.0 1,548,275 + -1.4 1,548,275 + +0.8 1,548,275 + -2.0 1,548,276 - +1.0 1,548,276 - +2.0 1,548,276 - -2.0 1,548,276 - -1.6 1,548,382 + -1.6 1,548,382 + -0.2 1,548,382 + +0.5 1,548,382 + -0.6
Or see this region's nucleotide sequence