Experiment: Varel_Bryant medium with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2690 and BT2691 overlap by 4 nucleotides BT2691 and BT2692 are separated by 21 nucleotides BT2692 and BT2693 are separated by 97 nucleotides
BT2690: BT2690 - histidine ammonia-lyase (NCBI ptt file), at 3,352,437 to 3,353,936
BT2690
BT2691: BT2691 - methenyltetrahydrofolate cyclohydrolase (NCBI ptt file), at 3,353,933 to 3,354,562
BT2691
BT2692: BT2692 - imidazolonepropionase (NCBI ptt file), at 3,354,584 to 3,355,837
BT2692
BT2693: BT2693 - formiminotransferase-cyclodeaminase (NCBI ptt file), at 3,355,935 to 3,356,837
BT2693
Position (kb)
3354
3355
3356 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3353.726 kb on + strand, within BT2690 at 3353.727 kb on - strand, within BT2690 at 3353.913 kb on + strand at 3353.913 kb on + strand at 3353.914 kb on - strand at 3353.936 kb on + strand at 3353.966 kb on + strand at 3353.967 kb on - strand at 3354.046 kb on + strand, within BT2691 at 3354.061 kb on + strand, within BT2691 at 3354.167 kb on + strand, within BT2691 at 3354.331 kb on + strand, within BT2691 at 3354.526 kb on + strand at 3354.526 kb on + strand at 3354.527 kb on - strand at 3354.543 kb on - strand at 3354.557 kb on + strand at 3354.566 kb on - strand at 3354.578 kb on + strand at 3354.599 kb on + strand at 3354.599 kb on + strand at 3354.600 kb on - strand at 3354.613 kb on + strand at 3354.614 kb on - strand at 3354.645 kb on + strand at 3354.646 kb on - strand at 3354.646 kb on - strand at 3354.787 kb on + strand, within BT2692 at 3354.798 kb on + strand, within BT2692 at 3354.798 kb on + strand, within BT2692 at 3354.802 kb on + strand, within BT2692 at 3354.915 kb on + strand, within BT2692 at 3354.956 kb on - strand at 3354.959 kb on + strand, within BT2692 at 3354.963 kb on - strand, within BT2692 at 3355.239 kb on + strand, within BT2692 at 3355.240 kb on - strand, within BT2692 at 3355.258 kb on + strand, within BT2692 at 3355.287 kb on - strand, within BT2692 at 3355.299 kb on - strand, within BT2692 at 3355.299 kb on - strand, within BT2692 at 3355.309 kb on + strand, within BT2692 at 3355.309 kb on + strand, within BT2692 at 3355.401 kb on - strand, within BT2692 at 3355.417 kb on + strand, within BT2692 at 3355.490 kb on + strand, within BT2692 at 3355.532 kb on - strand, within BT2692 at 3355.534 kb on - strand, within BT2692 at 3355.645 kb on - strand, within BT2692 at 3355.663 kb on - strand, within BT2692 at 3355.701 kb on + strand, within BT2692 at 3355.702 kb on - strand, within BT2692 at 3355.728 kb on - strand at 3355.743 kb on - strand at 3355.818 kb on - strand at 3355.819 kb on + strand at 3355.820 kb on - strand at 3355.839 kb on + strand at 3355.860 kb on + strand at 3355.916 kb on + strand at 3355.921 kb on + strand at 3355.922 kb on - strand at 3355.996 kb on + strand at 3356.010 kb on + strand at 3356.108 kb on - strand, within BT2693 at 3356.145 kb on + strand, within BT2693 at 3356.145 kb on + strand, within BT2693 at 3356.157 kb on - strand, within BT2693 at 3356.213 kb on + strand, within BT2693 at 3356.277 kb on - strand, within BT2693 at 3356.306 kb on + strand, within BT2693 at 3356.310 kb on + strand, within BT2693 at 3356.474 kb on + strand, within BT2693 at 3356.475 kb on - strand, within BT2693 at 3356.476 kb on + strand, within BT2693 at 3356.476 kb on + strand, within BT2693 at 3356.476 kb on + strand, within BT2693 at 3356.477 kb on - strand, within BT2693 at 3356.537 kb on + strand, within BT2693 at 3356.537 kb on + strand, within BT2693 at 3356.606 kb on + strand, within BT2693 at 3356.607 kb on - strand, within BT2693 at 3356.607 kb on - strand, within BT2693 at 3356.763 kb on + strand at 3356.763 kb on + strand at 3356.825 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Dimethyl Sulfoxide 2 vol% remove 3,353,726 + BT2690 0.86 -1.4 3,353,727 - BT2690 0.86 -0.1 3,353,913 + +1.0 3,353,913 + -0.7 3,353,914 - -1.1 3,353,936 + +0.0 3,353,966 + +0.0 3,353,967 - -0.1 3,354,046 + BT2691 0.18 -2.2 3,354,061 + BT2691 0.20 -0.9 3,354,167 + BT2691 0.37 -0.4 3,354,331 + BT2691 0.63 -0.1 3,354,526 + -1.2 3,354,526 + -0.9 3,354,527 - +0.6 3,354,543 - -0.6 3,354,557 + -1.6 3,354,566 - +2.0 3,354,578 + -1.1 3,354,599 + -0.2 3,354,599 + -1.0 3,354,600 - +0.0 3,354,613 + +0.7 3,354,614 - -1.4 3,354,645 + -1.3 3,354,646 - +1.0 3,354,646 - -1.3 3,354,787 + BT2692 0.16 -2.8 3,354,798 + BT2692 0.17 +0.4 3,354,798 + BT2692 0.17 +2.6 3,354,802 + BT2692 0.17 -0.8 3,354,915 + BT2692 0.26 -1.3 3,354,956 - -1.6 3,354,959 + BT2692 0.30 -2.1 3,354,963 - BT2692 0.30 -2.3 3,355,239 + BT2692 0.52 -0.6 3,355,240 - BT2692 0.52 +0.0 3,355,258 + BT2692 0.54 -0.2 3,355,287 - BT2692 0.56 +0.0 3,355,299 - BT2692 0.57 -1.0 3,355,299 - BT2692 0.57 -2.0 3,355,309 + BT2692 0.58 +0.0 3,355,309 + BT2692 0.58 -4.1 3,355,401 - BT2692 0.65 +1.0 3,355,417 + BT2692 0.66 +0.8 3,355,490 + BT2692 0.72 -1.2 3,355,532 - BT2692 0.76 -1.6 3,355,534 - BT2692 0.76 -1.0 3,355,645 - BT2692 0.85 -1.3 3,355,663 - BT2692 0.86 +0.7 3,355,701 + BT2692 0.89 -0.9 3,355,702 - BT2692 0.89 -0.5 3,355,728 - -1.3 3,355,743 - +1.0 3,355,818 - +0.2 3,355,819 + +1.0 3,355,820 - -1.2 3,355,839 + -0.1 3,355,860 + +1.6 3,355,916 + +2.0 3,355,921 + -0.8 3,355,922 - +0.0 3,355,996 + -1.6 3,356,010 + -1.3 3,356,108 - BT2693 0.19 +2.0 3,356,145 + BT2693 0.23 -1.3 3,356,145 + BT2693 0.23 -0.7 3,356,157 - BT2693 0.25 +1.0 3,356,213 + BT2693 0.31 -2.0 3,356,277 - BT2693 0.38 -0.7 3,356,306 + BT2693 0.41 +0.0 3,356,310 + BT2693 0.42 +2.6 3,356,474 + BT2693 0.60 +0.0 3,356,475 - BT2693 0.60 -0.6 3,356,476 + BT2693 0.60 -0.2 3,356,476 + BT2693 0.60 -0.4 3,356,476 + BT2693 0.60 -0.8 3,356,477 - BT2693 0.60 -2.8 3,356,537 + BT2693 0.67 -1.3 3,356,537 + BT2693 0.67 +0.0 3,356,606 + BT2693 0.74 +0.0 3,356,607 - BT2693 0.74 -1.3 3,356,607 - BT2693 0.74 -1.2 3,356,763 + -0.1 3,356,763 + +1.0 3,356,825 + +1.3
Or see this region's nucleotide sequence