Experiment: Chromium(III) Chloride Hexahydrate 0.000390625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1922 and Pf6N2E2_1923 are separated by 284 nucleotides Pf6N2E2_1923 and Pf6N2E2_1924 are separated by 418 nucleotides
Pf6N2E2_1922 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,275,029 to 2,277,140
_1922
Pf6N2E2_1923 - Acetyl-CoA acetyltransferase, at 2,277,425 to 2,278,594
_1923
Pf6N2E2_1924 - 6-phosphogluconolactonase (EC 3.1.1.31), at 2,279,013 to 2,280,185
_1924
Position (kb)
2277
2278
2279 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2276.572 kb on + strand, within Pf6N2E2_1922 at 2276.572 kb on + strand, within Pf6N2E2_1922 at 2276.631 kb on - strand, within Pf6N2E2_1922 at 2276.631 kb on - strand, within Pf6N2E2_1922 at 2276.631 kb on - strand, within Pf6N2E2_1922 at 2276.910 kb on - strand, within Pf6N2E2_1922 at 2276.942 kb on + strand at 2276.943 kb on - strand at 2277.420 kb on + strand at 2277.420 kb on + strand at 2277.432 kb on + strand at 2277.432 kb on + strand at 2277.433 kb on - strand at 2277.433 kb on - strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.447 kb on - strand at 2277.447 kb on - strand at 2277.447 kb on - strand at 2277.461 kb on + strand at 2277.461 kb on + strand at 2277.507 kb on + strand at 2277.507 kb on + strand at 2277.508 kb on - strand at 2277.508 kb on - strand at 2277.508 kb on - strand at 2277.606 kb on + strand, within Pf6N2E2_1923 at 2277.606 kb on + strand, within Pf6N2E2_1923 at 2277.606 kb on + strand, within Pf6N2E2_1923 at 2277.606 kb on + strand, within Pf6N2E2_1923 at 2277.607 kb on - strand, within Pf6N2E2_1923 at 2277.607 kb on - strand, within Pf6N2E2_1923 at 2277.607 kb on - strand, within Pf6N2E2_1923 at 2277.607 kb on - strand, within Pf6N2E2_1923 at 2277.623 kb on + strand, within Pf6N2E2_1923 at 2277.623 kb on + strand, within Pf6N2E2_1923 at 2277.798 kb on + strand, within Pf6N2E2_1923 at 2277.799 kb on - strand, within Pf6N2E2_1923 at 2277.839 kb on + strand, within Pf6N2E2_1923 at 2277.839 kb on + strand, within Pf6N2E2_1923 at 2277.839 kb on + strand, within Pf6N2E2_1923 at 2277.840 kb on - strand, within Pf6N2E2_1923 at 2277.840 kb on - strand, within Pf6N2E2_1923 at 2277.840 kb on - strand, within Pf6N2E2_1923 at 2277.842 kb on - strand, within Pf6N2E2_1923 at 2277.896 kb on + strand, within Pf6N2E2_1923 at 2278.002 kb on + strand, within Pf6N2E2_1923 at 2278.002 kb on + strand, within Pf6N2E2_1923 at 2278.002 kb on + strand, within Pf6N2E2_1923 at 2278.003 kb on - strand, within Pf6N2E2_1923 at 2278.079 kb on - strand, within Pf6N2E2_1923 at 2278.131 kb on + strand, within Pf6N2E2_1923 at 2278.188 kb on - strand, within Pf6N2E2_1923 at 2278.188 kb on - strand, within Pf6N2E2_1923 at 2278.268 kb on + strand, within Pf6N2E2_1923 at 2278.269 kb on - strand, within Pf6N2E2_1923 at 2278.296 kb on + strand, within Pf6N2E2_1923 at 2278.296 kb on + strand, within Pf6N2E2_1923 at 2278.417 kb on + strand, within Pf6N2E2_1923 at 2278.663 kb on - strand at 2278.846 kb on - strand at 2278.857 kb on + strand at 2278.857 kb on + strand at 2278.858 kb on - strand at 2278.858 kb on - strand at 2278.858 kb on - strand at 2278.858 kb on - strand at 2278.890 kb on - strand at 2279.034 kb on + strand at 2279.035 kb on - strand at 2279.045 kb on - strand at 2279.045 kb on - strand at 2279.064 kb on + strand at 2279.067 kb on - strand at 2279.133 kb on + strand, within Pf6N2E2_1924 at 2279.133 kb on + strand, within Pf6N2E2_1924 at 2279.134 kb on - strand, within Pf6N2E2_1924 at 2279.134 kb on - strand, within Pf6N2E2_1924 at 2279.166 kb on + strand, within Pf6N2E2_1924 at 2279.166 kb on + strand, within Pf6N2E2_1924 at 2279.167 kb on - strand, within Pf6N2E2_1924 at 2279.167 kb on - strand, within Pf6N2E2_1924 at 2279.167 kb on - strand, within Pf6N2E2_1924 at 2279.267 kb on - strand, within Pf6N2E2_1924 at 2279.294 kb on - strand, within Pf6N2E2_1924 at 2279.294 kb on - strand, within Pf6N2E2_1924 at 2279.303 kb on + strand, within Pf6N2E2_1924 at 2279.388 kb on - strand, within Pf6N2E2_1924 at 2279.436 kb on + strand, within Pf6N2E2_1924 at 2279.457 kb on + strand, within Pf6N2E2_1924 at 2279.482 kb on + strand, within Pf6N2E2_1924 at 2279.482 kb on + strand, within Pf6N2E2_1924
Per-strain Table
Position Strand Gene LocusTag Fraction Chromium(III) Chloride Hexahydrate 0.000390625 M remove 2,276,572 + Pf6N2E2_1922 0.73 -1.0 2,276,572 + Pf6N2E2_1922 0.73 +0.2 2,276,631 - Pf6N2E2_1922 0.76 +0.8 2,276,631 - Pf6N2E2_1922 0.76 +1.1 2,276,631 - Pf6N2E2_1922 0.76 -0.5 2,276,910 - Pf6N2E2_1922 0.89 +0.9 2,276,942 + -0.5 2,276,943 - -1.6 2,277,420 + +0.2 2,277,420 + -0.5 2,277,432 + -0.3 2,277,432 + +0.2 2,277,433 - +0.0 2,277,433 - +0.7 2,277,446 + -0.2 2,277,446 + -0.6 2,277,446 + -0.1 2,277,446 + -0.4 2,277,446 + +1.2 2,277,446 + -0.3 2,277,447 - +1.6 2,277,447 - +1.0 2,277,447 - -0.4 2,277,461 + +0.7 2,277,461 + -0.1 2,277,507 + +0.1 2,277,507 + +1.5 2,277,508 - -0.4 2,277,508 - -0.2 2,277,508 - -0.1 2,277,606 + Pf6N2E2_1923 0.15 -0.1 2,277,606 + Pf6N2E2_1923 0.15 -0.1 2,277,606 + Pf6N2E2_1923 0.15 -0.4 2,277,606 + Pf6N2E2_1923 0.15 -1.0 2,277,607 - Pf6N2E2_1923 0.16 -0.6 2,277,607 - Pf6N2E2_1923 0.16 -2.0 2,277,607 - Pf6N2E2_1923 0.16 -0.7 2,277,607 - Pf6N2E2_1923 0.16 +0.2 2,277,623 + Pf6N2E2_1923 0.17 -1.9 2,277,623 + Pf6N2E2_1923 0.17 -0.9 2,277,798 + Pf6N2E2_1923 0.32 -2.1 2,277,799 - Pf6N2E2_1923 0.32 +1.2 2,277,839 + Pf6N2E2_1923 0.35 -1.0 2,277,839 + Pf6N2E2_1923 0.35 +0.4 2,277,839 + Pf6N2E2_1923 0.35 -1.4 2,277,840 - Pf6N2E2_1923 0.35 -0.7 2,277,840 - Pf6N2E2_1923 0.35 +0.5 2,277,840 - Pf6N2E2_1923 0.35 -0.1 2,277,842 - Pf6N2E2_1923 0.36 +0.7 2,277,896 + Pf6N2E2_1923 0.40 +0.9 2,278,002 + Pf6N2E2_1923 0.49 +1.5 2,278,002 + Pf6N2E2_1923 0.49 -0.2 2,278,002 + Pf6N2E2_1923 0.49 +0.2 2,278,003 - Pf6N2E2_1923 0.49 -0.9 2,278,079 - Pf6N2E2_1923 0.56 -0.0 2,278,131 + Pf6N2E2_1923 0.60 +0.7 2,278,188 - Pf6N2E2_1923 0.65 -0.4 2,278,188 - Pf6N2E2_1923 0.65 -0.1 2,278,268 + Pf6N2E2_1923 0.72 +0.6 2,278,269 - Pf6N2E2_1923 0.72 -0.3 2,278,296 + Pf6N2E2_1923 0.74 -0.0 2,278,296 + Pf6N2E2_1923 0.74 -0.8 2,278,417 + Pf6N2E2_1923 0.85 +0.3 2,278,663 - -0.2 2,278,846 - +0.5 2,278,857 + +1.2 2,278,857 + +1.1 2,278,858 - +0.1 2,278,858 - +0.4 2,278,858 - +0.1 2,278,858 - +1.5 2,278,890 - +2.2 2,279,034 + +0.2 2,279,035 - -0.8 2,279,045 - -1.7 2,279,045 - +1.2 2,279,064 + -0.8 2,279,067 - -0.2 2,279,133 + Pf6N2E2_1924 0.10 -1.1 2,279,133 + Pf6N2E2_1924 0.10 +0.6 2,279,134 - Pf6N2E2_1924 0.10 +1.0 2,279,134 - Pf6N2E2_1924 0.10 -0.0 2,279,166 + Pf6N2E2_1924 0.13 -0.5 2,279,166 + Pf6N2E2_1924 0.13 -0.0 2,279,167 - Pf6N2E2_1924 0.13 -0.2 2,279,167 - Pf6N2E2_1924 0.13 +0.2 2,279,167 - Pf6N2E2_1924 0.13 -1.6 2,279,267 - Pf6N2E2_1924 0.22 +0.1 2,279,294 - Pf6N2E2_1924 0.24 +0.2 2,279,294 - Pf6N2E2_1924 0.24 +0.1 2,279,303 + Pf6N2E2_1924 0.25 +0.4 2,279,388 - Pf6N2E2_1924 0.32 -1.8 2,279,436 + Pf6N2E2_1924 0.36 +0.1 2,279,457 + Pf6N2E2_1924 0.38 -0.1 2,279,482 + Pf6N2E2_1924 0.40 +0.6 2,279,482 + Pf6N2E2_1924 0.40 +0.9
Or see this region's nucleotide sequence