Experiment: Manganese (II) chloride tetrahydrate 0.00078125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_356 and Pf6N2E2_357 are separated by 156 nucleotides Pf6N2E2_357 and Pf6N2E2_358 are separated by 21 nucleotides Pf6N2E2_358 and Pf6N2E2_359 are separated by 36 nucleotides
Pf6N2E2_356 - hypothetical protein, at 421,875 to 422,822
_356
Pf6N2E2_357 - hypothetical protein, at 422,979 to 423,353
_357
Pf6N2E2_358 - Haloacid dehalogenase, type II, at 423,375 to 424,043
_358
Pf6N2E2_359 - Opine oxidase subunit B, at 424,080 to 425,354
_359
Position (kb) 422
423
424 Strain fitness (log2 ratio) -1
0
1
2 at 423.018 kb on + strand, within Pf6N2E2_357 at 423.023 kb on + strand, within Pf6N2E2_357 at 423.023 kb on + strand, within Pf6N2E2_357 at 423.023 kb on + strand, within Pf6N2E2_357 at 423.137 kb on + strand, within Pf6N2E2_357 at 423.137 kb on + strand, within Pf6N2E2_357 at 423.138 kb on - strand, within Pf6N2E2_357 at 423.138 kb on - strand, within Pf6N2E2_357 at 423.282 kb on + strand, within Pf6N2E2_357 at 423.282 kb on + strand, within Pf6N2E2_357 at 423.283 kb on - strand, within Pf6N2E2_357 at 423.283 kb on - strand, within Pf6N2E2_357 at 423.396 kb on + strand at 423.396 kb on + strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.556 kb on - strand, within Pf6N2E2_358 at 423.633 kb on + strand, within Pf6N2E2_358 at 423.633 kb on + strand, within Pf6N2E2_358 at 423.781 kb on - strand, within Pf6N2E2_358 at 423.813 kb on + strand, within Pf6N2E2_358 at 424.095 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 0.00078125 M remove 423,018 + Pf6N2E2_357 0.10 +1.3 423,023 + Pf6N2E2_357 0.12 +2.5 423,023 + Pf6N2E2_357 0.12 +0.3 423,023 + Pf6N2E2_357 0.12 +1.0 423,137 + Pf6N2E2_357 0.42 +1.0 423,137 + Pf6N2E2_357 0.42 -1.6 423,138 - Pf6N2E2_357 0.42 +1.1 423,138 - Pf6N2E2_357 0.42 +0.6 423,282 + Pf6N2E2_357 0.81 +1.2 423,282 + Pf6N2E2_357 0.81 +0.5 423,283 - Pf6N2E2_357 0.81 +0.2 423,283 - Pf6N2E2_357 0.81 +0.5 423,396 + -1.2 423,396 + +0.4 423,397 - +0.6 423,397 - +0.3 423,397 - +1.0 423,397 - -1.5 423,397 - -0.8 423,397 - -0.2 423,556 - Pf6N2E2_358 0.27 +1.4 423,633 + Pf6N2E2_358 0.39 +0.0 423,633 + Pf6N2E2_358 0.39 -0.1 423,781 - Pf6N2E2_358 0.61 -1.5 423,813 + Pf6N2E2_358 0.65 +0.4 424,095 + -1.2
Or see this region's nucleotide sequence