Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT3033

Experiment: Varel_Bryant medium with Irgasan 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT3032 and BT3033 are separated by 5 nucleotidesBT3033 and BT3034 overlap by 4 nucleotidesBT3034 and BT3035 are separated by 105 nucleotides BT3032: BT3032 - conserved hypothetical protein, with a weak acyltransferase domain (NCBI ptt file), at 3,838,872 to 3,840,008 BT3032 BT3033: BT3033 - DNA topoisomerase IV subunit B (NCBI ptt file), at 3,840,014 to 3,841,891 BT3033 BT3034: BT3034 - phosphopantetheine adenylyltransferase (NCBI ptt file), at 3,841,888 to 3,842,343 BT3034 BT3035: BT3035 - carboxy-terminal processing protease precursor (NCBI ptt file), at 3,842,449 to 3,844,038 BT3035 Position (kb) 3840 3841 3842Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3840.013 kb on + strandat 3840.013 kb on + strandat 3840.013 kb on + strandat 3842.376 kb on - strandat 3842.376 kb on - strandat 3842.402 kb on - strandat 3842.472 kb on - strandat 3842.474 kb on - strandat 3842.594 kb on + strandat 3842.874 kb on + strand, within BT3035at 3842.875 kb on - strand, within BT3035

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant medium with Irgasan 0.0025 mM
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3,840,013 + -0.6
3,840,013 + +0.4
3,840,013 + +1.0
3,842,376 - -5.0
3,842,376 - -3.0
3,842,402 - -2.2
3,842,472 - -2.4
3,842,474 - -3.8
3,842,594 + +1.0
3,842,874 + BT3035 0.27 -2.5
3,842,875 - BT3035 0.27 -4.9

Or see this region's nucleotide sequence