Experiment: Manganese (II) chloride tetrahydrate 0.00078125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5473 and Pf6N2E2_5474 are separated by 198 nucleotides Pf6N2E2_5474 and Pf6N2E2_5475 are separated by 160 nucleotides
Pf6N2E2_5473 - hydrolase, alpha/beta fold family, at 6,289,509 to 6,290,528
_5473
Pf6N2E2_5474 - Glycine cleavage system transcriptional activator, at 6,290,727 to 6,291,608
_5474
Pf6N2E2_5475 - Acetolactate synthase, large subunit (EC 2.2.1.6), at 6,291,769 to 6,293,406
_5475
Position (kb)
6290
6291
6292 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6289.860 kb on + strand, within Pf6N2E2_5473 at 6289.860 kb on + strand, within Pf6N2E2_5473 at 6289.860 kb on + strand, within Pf6N2E2_5473 at 6289.861 kb on - strand, within Pf6N2E2_5473 at 6289.861 kb on - strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.930 kb on + strand, within Pf6N2E2_5473 at 6289.930 kb on + strand, within Pf6N2E2_5473 at 6289.930 kb on + strand, within Pf6N2E2_5473 at 6289.931 kb on - strand, within Pf6N2E2_5473 at 6290.196 kb on + strand, within Pf6N2E2_5473 at 6290.197 kb on - strand, within Pf6N2E2_5473 at 6290.197 kb on - strand, within Pf6N2E2_5473 at 6290.197 kb on - strand, within Pf6N2E2_5473 at 6290.224 kb on + strand, within Pf6N2E2_5473 at 6290.225 kb on - strand, within Pf6N2E2_5473 at 6290.225 kb on - strand, within Pf6N2E2_5473 at 6290.454 kb on + strand at 6290.455 kb on - strand at 6290.614 kb on + strand at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.641 kb on + strand at 6291.641 kb on + strand at 6291.642 kb on - strand at 6291.642 kb on - strand at 6291.642 kb on - strand at 6291.642 kb on - strand at 6291.642 kb on - strand at 6291.644 kb on + strand at 6291.645 kb on - strand at 6291.671 kb on - strand at 6291.745 kb on - strand at 6291.788 kb on + strand at 6291.788 kb on + strand at 6291.789 kb on - strand at 6291.800 kb on + strand at 6291.801 kb on - strand at 6291.801 kb on - strand at 6291.862 kb on + strand at 6291.863 kb on - strand at 6292.603 kb on + strand, within Pf6N2E2_5475
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 0.00078125 M remove 6,289,860 + Pf6N2E2_5473 0.34 -0.1 6,289,860 + Pf6N2E2_5473 0.34 -0.9 6,289,860 + Pf6N2E2_5473 0.34 +0.2 6,289,861 - Pf6N2E2_5473 0.35 -1.5 6,289,861 - Pf6N2E2_5473 0.35 -2.7 6,289,862 + Pf6N2E2_5473 0.35 +0.0 6,289,862 + Pf6N2E2_5473 0.35 +1.3 6,289,862 + Pf6N2E2_5473 0.35 -0.7 6,289,862 + Pf6N2E2_5473 0.35 +0.6 6,289,862 + Pf6N2E2_5473 0.35 +0.5 6,289,862 + Pf6N2E2_5473 0.35 -0.2 6,289,862 + Pf6N2E2_5473 0.35 +1.7 6,289,862 + Pf6N2E2_5473 0.35 -0.9 6,289,862 + Pf6N2E2_5473 0.35 -0.4 6,289,863 - Pf6N2E2_5473 0.35 +0.1 6,289,863 - Pf6N2E2_5473 0.35 +1.4 6,289,863 - Pf6N2E2_5473 0.35 +0.7 6,289,863 - Pf6N2E2_5473 0.35 -0.2 6,289,863 - Pf6N2E2_5473 0.35 +0.7 6,289,863 - Pf6N2E2_5473 0.35 +0.7 6,289,863 - Pf6N2E2_5473 0.35 +0.7 6,289,930 + Pf6N2E2_5473 0.41 +1.2 6,289,930 + Pf6N2E2_5473 0.41 -1.4 6,289,930 + Pf6N2E2_5473 0.41 +2.2 6,289,931 - Pf6N2E2_5473 0.41 -2.1 6,290,196 + Pf6N2E2_5473 0.67 -0.7 6,290,197 - Pf6N2E2_5473 0.67 -0.6 6,290,197 - Pf6N2E2_5473 0.67 -0.2 6,290,197 - Pf6N2E2_5473 0.67 +0.0 6,290,224 + Pf6N2E2_5473 0.70 -0.6 6,290,225 - Pf6N2E2_5473 0.70 +0.9 6,290,225 - Pf6N2E2_5473 0.70 -0.4 6,290,454 + -0.4 6,290,455 - -2.1 6,290,614 + -1.3 6,291,144 + Pf6N2E2_5474 0.47 +0.2 6,291,144 + Pf6N2E2_5474 0.47 +0.3 6,291,144 + Pf6N2E2_5474 0.47 +0.6 6,291,144 + Pf6N2E2_5474 0.47 +1.2 6,291,144 + Pf6N2E2_5474 0.47 -0.7 6,291,144 + Pf6N2E2_5474 0.47 +1.2 6,291,144 + Pf6N2E2_5474 0.47 -0.0 6,291,144 + Pf6N2E2_5474 0.47 +1.3 6,291,144 + Pf6N2E2_5474 0.47 -0.2 6,291,144 + Pf6N2E2_5474 0.47 -0.1 6,291,144 + Pf6N2E2_5474 0.47 -0.1 6,291,144 + Pf6N2E2_5474 0.47 +0.4 6,291,145 - Pf6N2E2_5474 0.47 +0.7 6,291,145 - Pf6N2E2_5474 0.47 -1.5 6,291,145 - Pf6N2E2_5474 0.47 +0.4 6,291,145 - Pf6N2E2_5474 0.47 +0.7 6,291,145 - Pf6N2E2_5474 0.47 +0.5 6,291,145 - Pf6N2E2_5474 0.47 +1.3 6,291,145 - Pf6N2E2_5474 0.47 -1.2 6,291,145 - Pf6N2E2_5474 0.47 +0.9 6,291,145 - Pf6N2E2_5474 0.47 +0.4 6,291,641 + +0.0 6,291,641 + -0.3 6,291,642 - -0.6 6,291,642 - +0.3 6,291,642 - -1.5 6,291,642 - -1.6 6,291,642 - +3.2 6,291,644 + -0.1 6,291,645 - -0.3 6,291,671 - -0.4 6,291,745 - -0.5 6,291,788 + -1.1 6,291,788 + -2.2 6,291,789 - -0.8 6,291,800 + +0.8 6,291,801 - +0.2 6,291,801 - +0.1 6,291,862 + -0.1 6,291,863 - -0.3 6,292,603 + Pf6N2E2_5475 0.51 -0.5
Or see this region's nucleotide sequence