Experiment: Manganese (II) chloride tetrahydrate 0.00078125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_348 and Pf6N2E2_349 overlap by 4 nucleotides Pf6N2E2_349 and Pf6N2E2_350 are separated by 65 nucleotides Pf6N2E2_350 and Pf6N2E2_351 are separated by 5 nucleotides
Pf6N2E2_348 - Transcriptional regulator, LysR family, at 414,114 to 415,043
_348
Pf6N2E2_349 - hypothetical protein, at 415,040 to 415,774
_349
Pf6N2E2_350 - Gfa-like protein, at 415,840 to 416,232
_350
Pf6N2E2_351 - Aldehyde dehydrogenase (EC 1.2.1.3), at 416,238 to 417,686
_351
Position (kb) 415
416 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 414.306 kb on + strand, within Pf6N2E2_348 at 414.306 kb on + strand, within Pf6N2E2_348 at 414.456 kb on + strand, within Pf6N2E2_348 at 414.457 kb on - strand, within Pf6N2E2_348 at 414.726 kb on + strand, within Pf6N2E2_348 at 414.727 kb on - strand, within Pf6N2E2_348 at 414.801 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.927 kb on + strand, within Pf6N2E2_348 at 414.927 kb on + strand, within Pf6N2E2_348 at 415.062 kb on + strand at 415.062 kb on + strand at 415.063 kb on - strand at 415.063 kb on - strand at 415.073 kb on + strand at 415.073 kb on + strand at 415.074 kb on - strand at 415.074 kb on - strand at 415.074 kb on - strand at 415.082 kb on + strand at 415.083 kb on - strand at 415.083 kb on - strand at 415.347 kb on + strand, within Pf6N2E2_349 at 415.515 kb on + strand, within Pf6N2E2_349 at 415.516 kb on - strand, within Pf6N2E2_349 at 415.542 kb on - strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.772 kb on + strand at 415.773 kb on - strand at 415.806 kb on + strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.865 kb on + strand at 416.131 kb on + strand, within Pf6N2E2_350 at 416.192 kb on - strand, within Pf6N2E2_350 at 416.231 kb on - strand at 416.231 kb on - strand at 416.272 kb on + strand at 416.272 kb on + strand at 416.272 kb on + strand at 416.297 kb on - strand at 416.323 kb on + strand at 416.449 kb on + strand, within Pf6N2E2_351 at 416.584 kb on + strand, within Pf6N2E2_351 at 416.584 kb on + strand, within Pf6N2E2_351 at 416.585 kb on - strand, within Pf6N2E2_351
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 0.00078125 M remove 414,306 + Pf6N2E2_348 0.21 +0.9 414,306 + Pf6N2E2_348 0.21 -0.4 414,456 + Pf6N2E2_348 0.37 +0.1 414,457 - Pf6N2E2_348 0.37 +0.4 414,726 + Pf6N2E2_348 0.66 +1.0 414,727 - Pf6N2E2_348 0.66 +1.1 414,801 + Pf6N2E2_348 0.74 +0.2 414,849 + Pf6N2E2_348 0.79 +2.2 414,849 + Pf6N2E2_348 0.79 +0.0 414,849 + Pf6N2E2_348 0.79 -0.4 414,849 + Pf6N2E2_348 0.79 +1.8 414,849 + Pf6N2E2_348 0.79 -1.1 414,850 - Pf6N2E2_348 0.79 +0.8 414,850 - Pf6N2E2_348 0.79 -0.2 414,850 - Pf6N2E2_348 0.79 +4.9 414,850 - Pf6N2E2_348 0.79 +0.0 414,927 + Pf6N2E2_348 0.87 +0.3 414,927 + Pf6N2E2_348 0.87 -0.8 415,062 + +0.9 415,062 + +0.8 415,063 - +0.1 415,063 - -0.2 415,073 + -0.4 415,073 + -0.7 415,074 - +0.8 415,074 - +1.8 415,074 - -0.2 415,082 + +1.5 415,083 - -0.9 415,083 - -0.2 415,347 + Pf6N2E2_349 0.42 -0.2 415,515 + Pf6N2E2_349 0.65 -0.4 415,516 - Pf6N2E2_349 0.65 -0.4 415,542 - Pf6N2E2_349 0.68 -1.6 415,660 + Pf6N2E2_349 0.84 -1.3 415,660 + Pf6N2E2_349 0.84 -0.1 415,772 + -0.6 415,773 - +1.6 415,806 + -0.2 415,807 - +1.1 415,807 - +0.6 415,807 - -0.3 415,807 - +1.6 415,865 + +1.8 416,131 + Pf6N2E2_350 0.74 -1.2 416,192 - Pf6N2E2_350 0.90 -1.1 416,231 - +0.4 416,231 - +0.8 416,272 + -0.2 416,272 + +0.6 416,272 + -2.1 416,297 - +0.9 416,323 + -0.8 416,449 + Pf6N2E2_351 0.15 -0.4 416,584 + Pf6N2E2_351 0.24 -2.2 416,584 + Pf6N2E2_351 0.24 -0.1 416,585 - Pf6N2E2_351 0.24 -0.6
Or see this region's nucleotide sequence