Experiment: Manganese (II) chloride tetrahydrate 0.00078125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3186 and Pf6N2E2_3187 are separated by 8 nucleotides Pf6N2E2_3187 and Pf6N2E2_3188 are separated by 24 nucleotides Pf6N2E2_3188 and Pf6N2E2_3189 are separated by 43 nucleotides Pf6N2E2_3189 and Pf6N2E2_3190 are separated by 50 nucleotides Pf6N2E2_3190 and Pf6N2E2_3191 are separated by 304 nucleotides
Pf6N2E2_3186 - Chaperone protein HscB, at 3,681,518 to 3,682,039
_3186
Pf6N2E2_3187 - Iron binding protein IscA for iron-sulfur cluster assembly, at 3,682,048 to 3,682,371
_3187
Pf6N2E2_3188 - Iron-sulfur cluster assembly scaffold protein IscU, at 3,682,396 to 3,682,782
_3188
Pf6N2E2_3189 - Cysteine desulfurase (EC 2.8.1.7), IscS subfamily, at 3,682,826 to 3,684,040
_3189
Pf6N2E2_3190 - Iron-sulfur cluster regulator IscR, at 3,684,091 to 3,684,543
_3190
Pf6N2E2_3191 - Serine acetyltransferase (EC 2.3.1.30), at 3,684,848 to 3,685,624
_3191
Position (kb)
3682
3683
3684
3685 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3684.139 kb on - strand, within Pf6N2E2_3190 at 3684.139 kb on - strand, within Pf6N2E2_3190 at 3684.194 kb on - strand, within Pf6N2E2_3190 at 3684.277 kb on - strand, within Pf6N2E2_3190 at 3684.277 kb on - strand, within Pf6N2E2_3190 at 3684.462 kb on - strand, within Pf6N2E2_3190 at 3684.571 kb on - strand at 3684.591 kb on - strand at 3684.697 kb on + strand at 3684.780 kb on + strand at 3684.780 kb on + strand at 3684.781 kb on - strand at 3684.781 kb on - strand at 3684.795 kb on + strand at 3684.849 kb on + strand at 3684.927 kb on + strand, within Pf6N2E2_3191 at 3684.928 kb on - strand, within Pf6N2E2_3191 at 3684.928 kb on - strand, within Pf6N2E2_3191 at 3684.931 kb on + strand, within Pf6N2E2_3191 at 3684.931 kb on + strand, within Pf6N2E2_3191 at 3684.932 kb on - strand, within Pf6N2E2_3191 at 3684.932 kb on - strand, within Pf6N2E2_3191 at 3684.932 kb on - strand, within Pf6N2E2_3191 at 3684.932 kb on - strand, within Pf6N2E2_3191
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 0.00078125 M remove 3,684,139 - Pf6N2E2_3190 0.11 -3.8 3,684,139 - Pf6N2E2_3190 0.11 +1.3 3,684,194 - Pf6N2E2_3190 0.23 +0.3 3,684,277 - Pf6N2E2_3190 0.41 +0.8 3,684,277 - Pf6N2E2_3190 0.41 -1.0 3,684,462 - Pf6N2E2_3190 0.82 -0.2 3,684,571 - +1.7 3,684,591 - +0.6 3,684,697 + -2.1 3,684,780 + -0.1 3,684,780 + +1.4 3,684,781 - +0.3 3,684,781 - +0.0 3,684,795 + -1.9 3,684,849 + +0.7 3,684,927 + Pf6N2E2_3191 0.10 +0.6 3,684,928 - Pf6N2E2_3191 0.10 -0.3 3,684,928 - Pf6N2E2_3191 0.10 +0.2 3,684,931 + Pf6N2E2_3191 0.11 +0.0 3,684,931 + Pf6N2E2_3191 0.11 -0.0 3,684,932 - Pf6N2E2_3191 0.11 -1.6 3,684,932 - Pf6N2E2_3191 0.11 +0.0 3,684,932 - Pf6N2E2_3191 0.11 +0.4 3,684,932 - Pf6N2E2_3191 0.11 -1.1
Or see this region's nucleotide sequence