Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_212

Experiment: Manganese (II) chloride tetrahydrate 0.00078125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_210 and Pf6N2E2_211 are separated by 92 nucleotidesPf6N2E2_211 and Pf6N2E2_212 overlap by 8 nucleotidesPf6N2E2_212 and Pf6N2E2_213 are separated by 48 nucleotidesPf6N2E2_213 and Pf6N2E2_214 are separated by 25 nucleotides Pf6N2E2_210 - Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40), at 264,376 to 267,099 _210 Pf6N2E2_211 - SanC, at 267,192 to 267,779 _211 Pf6N2E2_212 - Glycosyl transferase, group 2 family protein, at 267,772 to 269,946 _212 Pf6N2E2_213 - hypothetical protein, at 269,995 to 270,831 _213 Pf6N2E2_214 - dTDP-Rha:A-D-GlcNAc-diphosphoryl polyprenol, A-3-L-rhamnosyl transferase WbbL, at 270,857 to 271,807 _214 Position (kb) 267 268 269 270Strain fitness (log2 ratio) -2 -1 0 1 2at 267.097 kb on + strandat 267.097 kb on + strandat 267.097 kb on + strandat 267.097 kb on + strandat 267.098 kb on - strandat 267.098 kb on - strandat 267.098 kb on - strandat 267.098 kb on - strandat 267.256 kb on - strand, within Pf6N2E2_211at 267.256 kb on - strand, within Pf6N2E2_211at 267.256 kb on - strand, within Pf6N2E2_211at 267.261 kb on + strand, within Pf6N2E2_211at 267.261 kb on + strand, within Pf6N2E2_211at 267.261 kb on + strand, within Pf6N2E2_211at 267.261 kb on + strand, within Pf6N2E2_211at 267.261 kb on + strand, within Pf6N2E2_211at 267.262 kb on - strand, within Pf6N2E2_211at 267.262 kb on - strand, within Pf6N2E2_211at 267.262 kb on - strand, within Pf6N2E2_211at 267.504 kb on + strand, within Pf6N2E2_211at 267.505 kb on - strand, within Pf6N2E2_211at 267.623 kb on - strand, within Pf6N2E2_211at 267.706 kb on - strand, within Pf6N2E2_211at 267.777 kb on + strandat 267.777 kb on + strandat 267.778 kb on - strandat 267.850 kb on - strandat 268.153 kb on + strand, within Pf6N2E2_212at 268.153 kb on + strand, within Pf6N2E2_212at 268.153 kb on + strand, within Pf6N2E2_212at 268.154 kb on - strand, within Pf6N2E2_212at 268.154 kb on - strand, within Pf6N2E2_212at 268.154 kb on - strand, within Pf6N2E2_212at 268.154 kb on - strand, within Pf6N2E2_212at 268.193 kb on - strand, within Pf6N2E2_212at 268.258 kb on + strand, within Pf6N2E2_212at 268.271 kb on + strand, within Pf6N2E2_212at 268.362 kb on + strand, within Pf6N2E2_212at 268.362 kb on + strand, within Pf6N2E2_212at 268.369 kb on + strand, within Pf6N2E2_212at 268.423 kb on + strand, within Pf6N2E2_212at 268.423 kb on + strand, within Pf6N2E2_212at 268.423 kb on + strand, within Pf6N2E2_212at 268.423 kb on + strand, within Pf6N2E2_212at 268.537 kb on + strand, within Pf6N2E2_212at 268.538 kb on - strand, within Pf6N2E2_212at 268.666 kb on + strand, within Pf6N2E2_212at 268.841 kb on - strand, within Pf6N2E2_212at 268.919 kb on - strand, within Pf6N2E2_212at 268.925 kb on - strand, within Pf6N2E2_212at 268.925 kb on - strand, within Pf6N2E2_212at 268.925 kb on - strand, within Pf6N2E2_212at 268.994 kb on - strand, within Pf6N2E2_212at 268.994 kb on - strand, within Pf6N2E2_212at 269.065 kb on + strand, within Pf6N2E2_212at 269.066 kb on - strand, within Pf6N2E2_212at 269.066 kb on - strand, within Pf6N2E2_212at 269.066 kb on - strand, within Pf6N2E2_212at 269.066 kb on - strand, within Pf6N2E2_212at 269.066 kb on - strand, within Pf6N2E2_212at 269.176 kb on + strand, within Pf6N2E2_212at 269.176 kb on + strand, within Pf6N2E2_212at 269.176 kb on + strand, within Pf6N2E2_212at 269.176 kb on + strand, within Pf6N2E2_212at 269.177 kb on - strand, within Pf6N2E2_212at 269.227 kb on - strand, within Pf6N2E2_212at 269.429 kb on + strand, within Pf6N2E2_212at 269.430 kb on - strand, within Pf6N2E2_212at 269.514 kb on - strandat 269.720 kb on - strand, within Pf6N2E2_212at 269.827 kb on + strandat 269.858 kb on - strandat 270.297 kb on + strand, within Pf6N2E2_213at 270.297 kb on + strand, within Pf6N2E2_213at 270.297 kb on + strand, within Pf6N2E2_213at 270.298 kb on - strand, within Pf6N2E2_213at 270.298 kb on - strand, within Pf6N2E2_213at 270.298 kb on - strand, within Pf6N2E2_213at 270.298 kb on - strand, within Pf6N2E2_213at 270.298 kb on - strand, within Pf6N2E2_213at 270.298 kb on - strand, within Pf6N2E2_213at 270.443 kb on + strand, within Pf6N2E2_213at 270.443 kb on + strand, within Pf6N2E2_213at 270.444 kb on - strand, within Pf6N2E2_213at 270.444 kb on - strand, within Pf6N2E2_213at 270.444 kb on - strand, within Pf6N2E2_213at 270.444 kb on - strand, within Pf6N2E2_213at 270.444 kb on - strand, within Pf6N2E2_213at 270.638 kb on + strand, within Pf6N2E2_213at 270.832 kb on + strandat 270.858 kb on + strandat 270.858 kb on + strandat 270.859 kb on - strandat 270.863 kb on + strandat 270.864 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 0.00078125 M
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267,097 + +0.3
267,097 + -0.9
267,097 + +0.6
267,097 + +0.1
267,098 - -0.8
267,098 - -0.5
267,098 - +1.9
267,098 - -1.6
267,256 - Pf6N2E2_211 0.11 -1.6
267,256 - Pf6N2E2_211 0.11 +1.1
267,256 - Pf6N2E2_211 0.11 -1.1
267,261 + Pf6N2E2_211 0.12 +0.9
267,261 + Pf6N2E2_211 0.12 -0.8
267,261 + Pf6N2E2_211 0.12 -0.4
267,261 + Pf6N2E2_211 0.12 +0.5
267,261 + Pf6N2E2_211 0.12 +0.3
267,262 - Pf6N2E2_211 0.12 -1.4
267,262 - Pf6N2E2_211 0.12 -1.0
267,262 - Pf6N2E2_211 0.12 +0.9
267,504 + Pf6N2E2_211 0.53 +0.0
267,505 - Pf6N2E2_211 0.53 +0.7
267,623 - Pf6N2E2_211 0.73 +0.2
267,706 - Pf6N2E2_211 0.87 +1.5
267,777 + -1.8
267,777 + -1.6
267,778 - +1.6
267,850 - -1.8
268,153 + Pf6N2E2_212 0.18 -0.4
268,153 + Pf6N2E2_212 0.18 -0.5
268,153 + Pf6N2E2_212 0.18 -0.2
268,154 - Pf6N2E2_212 0.18 -1.7
268,154 - Pf6N2E2_212 0.18 +0.9
268,154 - Pf6N2E2_212 0.18 -1.2
268,154 - Pf6N2E2_212 0.18 -0.6
268,193 - Pf6N2E2_212 0.19 -1.6
268,258 + Pf6N2E2_212 0.22 +0.3
268,271 + Pf6N2E2_212 0.23 +0.2
268,362 + Pf6N2E2_212 0.27 +0.3
268,362 + Pf6N2E2_212 0.27 -0.7
268,369 + Pf6N2E2_212 0.27 +0.1
268,423 + Pf6N2E2_212 0.30 +0.1
268,423 + Pf6N2E2_212 0.30 -0.4
268,423 + Pf6N2E2_212 0.30 +0.0
268,423 + Pf6N2E2_212 0.30 -0.1
268,537 + Pf6N2E2_212 0.35 +0.0
268,538 - Pf6N2E2_212 0.35 +0.0
268,666 + Pf6N2E2_212 0.41 +1.1
268,841 - Pf6N2E2_212 0.49 -1.4
268,919 - Pf6N2E2_212 0.53 +0.0
268,925 - Pf6N2E2_212 0.53 -0.3
268,925 - Pf6N2E2_212 0.53 -0.6
268,925 - Pf6N2E2_212 0.53 -0.8
268,994 - Pf6N2E2_212 0.56 -1.1
268,994 - Pf6N2E2_212 0.56 -0.6
269,065 + Pf6N2E2_212 0.59 -0.5
269,066 - Pf6N2E2_212 0.59 +0.4
269,066 - Pf6N2E2_212 0.59 +1.0
269,066 - Pf6N2E2_212 0.59 -0.8
269,066 - Pf6N2E2_212 0.59 +0.2
269,066 - Pf6N2E2_212 0.59 +0.9
269,176 + Pf6N2E2_212 0.65 -0.2
269,176 + Pf6N2E2_212 0.65 +0.8
269,176 + Pf6N2E2_212 0.65 -0.4
269,176 + Pf6N2E2_212 0.65 -0.5
269,177 - Pf6N2E2_212 0.65 +0.3
269,227 - Pf6N2E2_212 0.67 +0.6
269,429 + Pf6N2E2_212 0.76 +0.1
269,430 - Pf6N2E2_212 0.76 +1.3
269,514 - -0.4
269,720 - Pf6N2E2_212 0.90 -1.0
269,827 + +0.5
269,858 - +1.1
270,297 + Pf6N2E2_213 0.36 +0.8
270,297 + Pf6N2E2_213 0.36 -0.1
270,297 + Pf6N2E2_213 0.36 +0.8
270,298 - Pf6N2E2_213 0.36 -0.6
270,298 - Pf6N2E2_213 0.36 -0.2
270,298 - Pf6N2E2_213 0.36 +0.3
270,298 - Pf6N2E2_213 0.36 +1.3
270,298 - Pf6N2E2_213 0.36 -0.7
270,298 - Pf6N2E2_213 0.36 +0.7
270,443 + Pf6N2E2_213 0.54 -0.2
270,443 + Pf6N2E2_213 0.54 +0.4
270,444 - Pf6N2E2_213 0.54 +0.1
270,444 - Pf6N2E2_213 0.54 +0.2
270,444 - Pf6N2E2_213 0.54 +0.1
270,444 - Pf6N2E2_213 0.54 -1.0
270,444 - Pf6N2E2_213 0.54 +0.0
270,638 + Pf6N2E2_213 0.77 -0.3
270,832 + -0.3
270,858 + -0.8
270,858 + +1.1
270,859 - -0.2
270,863 + -0.4
270,864 - +1.3

Or see this region's nucleotide sequence