Experiment: Varel_Bryant medium with Irgasan 0.005 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1281 and BT1282 are separated by 23 nucleotides BT1282 and BT1283 are separated by 18 nucleotides
BT1281: BT1281 - hypothetical protein (NCBI ptt file), at 1,593,871 to 1,595,466
BT1281
BT1282: BT1282 - hypothetical protein (NCBI ptt file), at 1,595,490 to 1,596,473
BT1282
BT1283: BT1283 - conserved hypothetical protein (NCBI ptt file), at 1,596,492 to 1,597,814
BT1283
Position (kb)
1595
1596
1597 Strain fitness (log2 ratio)
-2
-1
0
1 at 1594.551 kb on - strand, within BT1281 at 1594.568 kb on + strand, within BT1281 at 1594.569 kb on - strand, within BT1281 at 1594.779 kb on + strand, within BT1281 at 1594.795 kb on - strand, within BT1281 at 1594.833 kb on - strand, within BT1281 at 1594.863 kb on - strand, within BT1281 at 1594.871 kb on - strand, within BT1281 at 1594.904 kb on - strand, within BT1281 at 1594.907 kb on + strand, within BT1281 at 1595.083 kb on - strand, within BT1281 at 1595.104 kb on - strand, within BT1281 at 1595.128 kb on - strand, within BT1281 at 1595.181 kb on + strand, within BT1281 at 1595.182 kb on - strand, within BT1281 at 1595.299 kb on - strand, within BT1281 at 1595.330 kb on - strand at 1595.380 kb on - strand at 1595.385 kb on - strand at 1595.390 kb on + strand at 1595.390 kb on + strand at 1595.403 kb on + strand at 1595.439 kb on - strand at 1595.452 kb on - strand at 1595.464 kb on + strand at 1595.465 kb on - strand at 1595.509 kb on + strand at 1595.608 kb on + strand, within BT1282 at 1595.639 kb on - strand, within BT1282 at 1595.639 kb on - strand, within BT1282 at 1595.650 kb on - strand, within BT1282 at 1595.689 kb on - strand, within BT1282 at 1595.689 kb on - strand, within BT1282 at 1595.720 kb on + strand, within BT1282 at 1595.802 kb on - strand, within BT1282 at 1596.045 kb on - strand, within BT1282 at 1596.065 kb on - strand, within BT1282 at 1596.157 kb on + strand, within BT1282 at 1596.169 kb on - strand, within BT1282 at 1596.173 kb on - strand, within BT1282 at 1596.180 kb on - strand, within BT1282 at 1596.182 kb on + strand, within BT1282 at 1596.182 kb on + strand, within BT1282 at 1596.182 kb on + strand at 1596.209 kb on - strand, within BT1282 at 1596.235 kb on - strand, within BT1282 at 1596.246 kb on - strand, within BT1282 at 1596.286 kb on + strand, within BT1282 at 1596.303 kb on - strand, within BT1282 at 1596.399 kb on + strand at 1596.422 kb on + strand at 1596.532 kb on + strand at 1596.601 kb on + strand at 1596.710 kb on + strand, within BT1283 at 1596.722 kb on - strand, within BT1283 at 1596.722 kb on - strand, within BT1283 at 1596.743 kb on - strand, within BT1283 at 1596.779 kb on + strand, within BT1283 at 1596.779 kb on + strand, within BT1283 at 1596.780 kb on - strand, within BT1283 at 1596.780 kb on - strand, within BT1283 at 1596.827 kb on - strand, within BT1283 at 1596.947 kb on - strand, within BT1283 at 1596.972 kb on + strand, within BT1283 at 1596.972 kb on + strand, within BT1283 at 1596.972 kb on + strand, within BT1283 at 1596.972 kb on + strand, within BT1283 at 1597.133 kb on + strand, within BT1283 at 1597.158 kb on - strand, within BT1283 at 1597.327 kb on - strand, within BT1283 at 1597.417 kb on + strand, within BT1283
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Irgasan 0.005 mM remove 1,594,551 - BT1281 0.43 +1.1 1,594,568 + BT1281 0.44 -2.3 1,594,569 - BT1281 0.44 -0.2 1,594,779 + BT1281 0.57 +0.1 1,594,795 - BT1281 0.58 +1.7 1,594,833 - BT1281 0.60 +0.5 1,594,863 - BT1281 0.62 -0.1 1,594,871 - BT1281 0.63 -0.3 1,594,904 - BT1281 0.65 -0.5 1,594,907 + BT1281 0.65 -0.5 1,595,083 - BT1281 0.76 -0.5 1,595,104 - BT1281 0.77 -2.4 1,595,128 - BT1281 0.79 -1.5 1,595,181 + BT1281 0.82 +1.1 1,595,182 - BT1281 0.82 -1.6 1,595,299 - BT1281 0.89 +1.5 1,595,330 - -2.5 1,595,380 - -2.1 1,595,385 - -1.1 1,595,390 + +1.1 1,595,390 + -1.8 1,595,403 + -0.3 1,595,439 - -0.9 1,595,452 - -1.5 1,595,464 + -0.2 1,595,465 - -0.9 1,595,509 + -1.8 1,595,608 + BT1282 0.12 +0.2 1,595,639 - BT1282 0.15 +0.4 1,595,639 - BT1282 0.15 -1.2 1,595,650 - BT1282 0.16 -0.5 1,595,689 - BT1282 0.20 +0.0 1,595,689 - BT1282 0.20 +1.7 1,595,720 + BT1282 0.23 +0.1 1,595,802 - BT1282 0.32 -1.6 1,596,045 - BT1282 0.56 +0.0 1,596,065 - BT1282 0.58 -1.8 1,596,157 + BT1282 0.68 +0.5 1,596,169 - BT1282 0.69 -2.4 1,596,173 - BT1282 0.69 -0.6 1,596,180 - BT1282 0.70 -0.9 1,596,182 + BT1282 0.70 +1.7 1,596,182 + BT1282 0.70 +0.5 1,596,182 + +0.1 1,596,209 - BT1282 0.73 +1.1 1,596,235 - BT1282 0.76 -0.5 1,596,246 - BT1282 0.77 -0.3 1,596,286 + BT1282 0.81 +0.4 1,596,303 - BT1282 0.83 +1.1 1,596,399 + +1.1 1,596,422 + -1.2 1,596,532 + +1.1 1,596,601 + +0.5 1,596,710 + BT1283 0.16 +0.1 1,596,722 - BT1283 0.17 -0.8 1,596,722 - BT1283 0.17 -1.8 1,596,743 - BT1283 0.19 -2.0 1,596,779 + BT1283 0.22 -1.7 1,596,779 + BT1283 0.22 -0.5 1,596,780 - BT1283 0.22 +0.1 1,596,780 - BT1283 0.22 -0.5 1,596,827 - BT1283 0.25 -1.3 1,596,947 - BT1283 0.34 -0.5 1,596,972 + BT1283 0.36 -0.5 1,596,972 + BT1283 0.36 +0.4 1,596,972 + BT1283 0.36 +0.5 1,596,972 + BT1283 0.36 -0.1 1,597,133 + BT1283 0.48 +0.1 1,597,158 - BT1283 0.50 -0.7 1,597,327 - BT1283 0.63 -1.7 1,597,417 + BT1283 0.70 -0.8
Or see this region's nucleotide sequence