Experiment: ALP with 3 all (C)s
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0017 and Dsui_0018 overlap by 1 nucleotides Dsui_0018 and Dsui_0019 are separated by 52 nucleotides Dsui_0019 and Dsui_0020 are separated by 110 nucleotides Dsui_0020 and Dsui_0021 overlap by 8 nucleotides
Dsui_0017: Dsui_0017 - 2-haloalkanoic acid dehalogenase, type II, at 15,031 to 15,720
_0017
Dsui_0018: Dsui_0018 - putative permease, at 15,720 to 16,505
_0018
Dsui_0019: Dsui_0019 - malate synthase A, at 16,558 to 18,156
_0019
Dsui_0020: Dsui_0020 - HAD-superfamily subfamily IIA hydrolase, TIGR01458, at 18,267 to 19,067
_0020
Dsui_0021: Dsui_0021 - putative metalloendopeptidase, at 19,060 to 19,293
_0021
Position (kb) 16
17
18
19 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 15.654 kb on + strand at 15.667 kb on - strand at 15.692 kb on - strand at 15.918 kb on - strand, within Dsui_0018 at 15.931 kb on + strand, within Dsui_0018 at 15.940 kb on - strand, within Dsui_0018 at 16.033 kb on - strand, within Dsui_0018 at 16.276 kb on - strand at 16.319 kb on - strand, within Dsui_0018 at 16.359 kb on - strand, within Dsui_0018 at 16.448 kb on + strand at 16.467 kb on + strand at 16.487 kb on + strand at 16.544 kb on + strand at 16.544 kb on + strand at 16.548 kb on + strand at 16.552 kb on - strand at 16.562 kb on + strand at 16.562 kb on + strand at 16.564 kb on - strand at 16.570 kb on - strand at 16.595 kb on + strand at 16.659 kb on + strand at 16.695 kb on - strand at 16.788 kb on - strand, within Dsui_0019 at 16.850 kb on - strand, within Dsui_0019 at 16.919 kb on + strand, within Dsui_0019 at 16.950 kb on + strand, within Dsui_0019 at 17.046 kb on - strand, within Dsui_0019 at 17.065 kb on - strand at 17.276 kb on + strand, within Dsui_0019 at 17.284 kb on - strand, within Dsui_0019 at 17.303 kb on - strand, within Dsui_0019 at 17.336 kb on + strand, within Dsui_0019 at 17.432 kb on + strand, within Dsui_0019 at 17.480 kb on + strand, within Dsui_0019 at 17.488 kb on - strand, within Dsui_0019 at 17.488 kb on - strand, within Dsui_0019 at 17.527 kb on + strand, within Dsui_0019 at 17.742 kb on - strand, within Dsui_0019 at 17.795 kb on + strand, within Dsui_0019 at 17.891 kb on - strand, within Dsui_0019 at 17.915 kb on + strand, within Dsui_0019 at 18.020 kb on + strand at 18.048 kb on - strand at 18.098 kb on + strand at 18.120 kb on + strand at 18.145 kb on + strand at 18.146 kb on - strand at 18.151 kb on + strand at 18.216 kb on + strand at 18.224 kb on - strand at 18.231 kb on - strand at 18.231 kb on - strand at 18.233 kb on + strand at 18.233 kb on + strand at 18.278 kb on + strand at 18.284 kb on + strand at 18.290 kb on - strand at 18.330 kb on - strand at 18.391 kb on + strand at 18.507 kb on + strand at 18.515 kb on - strand, within Dsui_0020 at 18.527 kb on + strand, within Dsui_0020 at 18.580 kb on - strand, within Dsui_0020 at 18.580 kb on - strand, within Dsui_0020 at 18.633 kb on + strand, within Dsui_0020 at 18.727 kb on + strand, within Dsui_0020 at 18.845 kb on + strand, within Dsui_0020 at 18.881 kb on - strand, within Dsui_0020 at 18.939 kb on + strand at 18.947 kb on - strand, within Dsui_0020 at 18.950 kb on + strand at 18.988 kb on + strand at 18.988 kb on + strand at 19.032 kb on + strand at 19.059 kb on - strand at 19.095 kb on + strand, within Dsui_0021 at 19.102 kb on - strand, within Dsui_0021
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with 3 all (C)s remove 15,654 + +0.6 15,667 - +1.1 15,692 - +1.7 15,918 - Dsui_0018 0.25 -1.0 15,931 + Dsui_0018 0.27 -0.1 15,940 - Dsui_0018 0.28 +0.6 16,033 - Dsui_0018 0.40 +0.4 16,276 - +0.0 16,319 - Dsui_0018 0.76 +0.0 16,359 - Dsui_0018 0.81 +1.0 16,448 + +0.5 16,467 + +0.0 16,487 + -1.0 16,544 + -0.4 16,544 + +0.0 16,548 + +0.0 16,552 - +0.0 16,562 + +1.0 16,562 + -1.2 16,564 - +0.0 16,570 - +1.0 16,595 + -1.2 16,659 + +0.2 16,695 - +2.0 16,788 - Dsui_0019 0.14 -0.6 16,850 - Dsui_0019 0.18 -1.0 16,919 + Dsui_0019 0.23 +0.2 16,950 + Dsui_0019 0.25 -0.5 17,046 - Dsui_0019 0.31 -0.6 17,065 - +1.0 17,276 + Dsui_0019 0.45 +0.4 17,284 - Dsui_0019 0.45 -1.0 17,303 - Dsui_0019 0.47 -0.7 17,336 + Dsui_0019 0.49 +2.0 17,432 + Dsui_0019 0.55 -1.2 17,480 + Dsui_0019 0.58 -0.3 17,488 - Dsui_0019 0.58 +0.3 17,488 - Dsui_0019 0.58 +0.4 17,527 + Dsui_0019 0.61 +0.3 17,742 - Dsui_0019 0.74 -3.0 17,795 + Dsui_0019 0.77 +0.0 17,891 - Dsui_0019 0.83 +0.5 17,915 + Dsui_0019 0.85 -1.0 18,020 + -0.9 18,048 - -1.0 18,098 + +0.1 18,120 + +0.2 18,145 + +0.9 18,146 - +0.0 18,151 + -0.7 18,216 + -0.3 18,224 - +0.0 18,231 - +0.6 18,231 - +0.0 18,233 + +0.6 18,233 + -1.0 18,278 + +0.4 18,284 + -0.1 18,290 - -0.5 18,330 - +0.8 18,391 + +1.0 18,507 + -0.3 18,515 - Dsui_0020 0.31 -0.6 18,527 + Dsui_0020 0.32 -0.4 18,580 - Dsui_0020 0.39 +0.0 18,580 - Dsui_0020 0.39 +0.1 18,633 + Dsui_0020 0.46 +0.7 18,727 + Dsui_0020 0.57 +1.4 18,845 + Dsui_0020 0.72 +0.4 18,881 - Dsui_0020 0.77 +0.4 18,939 + +0.0 18,947 - Dsui_0020 0.85 +0.6 18,950 + +1.6 18,988 + +0.3 18,988 + +0.0 19,032 + -1.0 19,059 - -0.6 19,095 + Dsui_0021 0.15 -0.6 19,102 - Dsui_0021 0.18 +0.4
Or see this region's nucleotide sequence