Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23985 and BWI76_RS23990 are separated by 377 nucleotides BWI76_RS23990 and BWI76_RS23995 are separated by 24 nucleotides BWI76_RS23995 and BWI76_RS24000 are separated by 0 nucleotides BWI76_RS24000 and BWI76_RS24005 overlap by 13 nucleotides
BWI76_RS23985: BWI76_RS23985 - erythrose-4-phosphate dehydrogenase, at 5,006,463 to 5,007,482
_RS23985
BWI76_RS23990: BWI76_RS23990 - DNA-binding protein, at 5,007,860 to 5,008,285
_RS23990
BWI76_RS23995: BWI76_RS23995 - hypothetical protein, at 5,008,310 to 5,008,888
_RS23995
BWI76_RS24000: BWI76_RS24000 - hypothetical protein, at 5,008,889 to 5,009,596
_RS24000
BWI76_RS24005: BWI76_RS24005 - cobalt ABC transporter ATP-binding protein, at 5,009,584 to 5,010,261
_RS24005
Position (kb)
5008
5009 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5007.371 kb on + strand, within BWI76_RS23985 at 5007.458 kb on - strand at 5007.490 kb on + strand at 5007.490 kb on + strand at 5007.524 kb on + strand at 5007.532 kb on - strand at 5007.532 kb on - strand at 5007.539 kb on + strand at 5007.547 kb on - strand at 5007.625 kb on - strand at 5007.875 kb on + strand at 5007.897 kb on + strand at 5007.902 kb on - strand at 5007.958 kb on + strand, within BWI76_RS23990 at 5008.024 kb on - strand, within BWI76_RS23990 at 5008.155 kb on - strand, within BWI76_RS23990 at 5008.170 kb on + strand, within BWI76_RS23990 at 5008.178 kb on - strand, within BWI76_RS23990 at 5008.188 kb on + strand, within BWI76_RS23990 at 5008.196 kb on - strand, within BWI76_RS23990 at 5008.756 kb on - strand, within BWI76_RS23995 at 5008.877 kb on + strand at 5008.913 kb on - strand at 5008.932 kb on + strand at 5008.950 kb on - strand at 5008.961 kb on + strand, within BWI76_RS24000 at 5008.961 kb on + strand, within BWI76_RS24000 at 5008.970 kb on - strand, within BWI76_RS24000 at 5008.977 kb on - strand, within BWI76_RS24000 at 5008.984 kb on + strand, within BWI76_RS24000 at 5008.984 kb on + strand, within BWI76_RS24000 at 5009.061 kb on + strand, within BWI76_RS24000 at 5009.069 kb on - strand, within BWI76_RS24000 at 5009.284 kb on + strand, within BWI76_RS24000 at 5009.310 kb on - strand, within BWI76_RS24000 at 5009.415 kb on + strand, within BWI76_RS24000 at 5009.525 kb on - strand, within BWI76_RS24000 at 5009.528 kb on - strand at 5009.591 kb on + strand at 5009.591 kb on + strand at 5009.721 kb on + strand, within BWI76_RS24005 at 5009.729 kb on - strand, within BWI76_RS24005 at 5009.828 kb on - strand, within BWI76_RS24005 at 5009.828 kb on - strand, within BWI76_RS24005
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 5,007,371 + BWI76_RS23985 0.89 -1.8 5,007,458 - +0.6 5,007,490 + -0.7 5,007,490 + +0.8 5,007,524 + -0.3 5,007,532 - +0.2 5,007,532 - +0.8 5,007,539 + -0.8 5,007,547 - +0.3 5,007,625 - +0.3 5,007,875 + +2.4 5,007,897 + -0.2 5,007,902 - +1.1 5,007,958 + BWI76_RS23990 0.23 +0.2 5,008,024 - BWI76_RS23990 0.38 -1.0 5,008,155 - BWI76_RS23990 0.69 +0.6 5,008,170 + BWI76_RS23990 0.73 +0.1 5,008,178 - BWI76_RS23990 0.75 -0.3 5,008,188 + BWI76_RS23990 0.77 -0.6 5,008,196 - BWI76_RS23990 0.79 -1.1 5,008,756 - BWI76_RS23995 0.77 +0.3 5,008,877 + -0.3 5,008,913 - +0.4 5,008,932 + -1.5 5,008,950 - +0.3 5,008,961 + BWI76_RS24000 0.10 +0.2 5,008,961 + BWI76_RS24000 0.10 -1.0 5,008,970 - BWI76_RS24000 0.11 -0.1 5,008,977 - BWI76_RS24000 0.12 +0.3 5,008,984 + BWI76_RS24000 0.13 +0.4 5,008,984 + BWI76_RS24000 0.13 -0.5 5,009,061 + BWI76_RS24000 0.24 -1.4 5,009,069 - BWI76_RS24000 0.25 +0.5 5,009,284 + BWI76_RS24000 0.56 +0.1 5,009,310 - BWI76_RS24000 0.59 +0.6 5,009,415 + BWI76_RS24000 0.74 -0.4 5,009,525 - BWI76_RS24000 0.90 +0.4 5,009,528 - +1.0 5,009,591 + -1.9 5,009,591 + -1.0 5,009,721 + BWI76_RS24005 0.20 +0.3 5,009,729 - BWI76_RS24005 0.21 +0.9 5,009,828 - BWI76_RS24005 0.36 -0.2 5,009,828 - BWI76_RS24005 0.36 -0.2
Or see this region's nucleotide sequence