Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21125

Experiment: Paraquat dichloride 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21115 and BWI76_RS21120 are separated by 32 nucleotidesBWI76_RS21120 and BWI76_RS21125 are separated by 96 nucleotidesBWI76_RS21125 and BWI76_RS21130 are separated by 107 nucleotidesBWI76_RS21130 and BWI76_RS21135 are separated by 1 nucleotidesBWI76_RS21135 and BWI76_RS21140 are separated by 1 nucleotides BWI76_RS21115: BWI76_RS21115 - 3-mercaptopyruvate sulfurtransferase, at 4,423,161 to 4,424,006 _RS21115 BWI76_RS21120: BWI76_RS21120 - enhanced serine sensitivity protein SseB, at 4,424,039 to 4,424,815 _RS21120 BWI76_RS21125: BWI76_RS21125 - aminopeptidase PepB, at 4,424,912 to 4,426,198 _RS21125 BWI76_RS21130: BWI76_RS21130 - Fe-S assembly protein IscX, at 4,426,306 to 4,426,506 _RS21130 BWI76_RS21135: BWI76_RS21135 - ferredoxin, 2Fe-2S type, ISC system, at 4,426,508 to 4,426,843 _RS21135 BWI76_RS21140: BWI76_RS21140 - molecular chaperone HscA, at 4,426,845 to 4,428,695 _RS21140 Position (kb) 4424 4425 4426 4427Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4423.980 kb on + strandat 4424.282 kb on + strand, within BWI76_RS21120at 4424.282 kb on + strand, within BWI76_RS21120at 4424.282 kb on + strand, within BWI76_RS21120at 4424.290 kb on - strand, within BWI76_RS21120at 4424.290 kb on - strandat 4424.292 kb on - strand, within BWI76_RS21120at 4424.384 kb on - strand, within BWI76_RS21120at 4424.529 kb on + strand, within BWI76_RS21120at 4424.681 kb on - strand, within BWI76_RS21120at 4425.034 kb on - strandat 4425.226 kb on + strand, within BWI76_RS21125at 4425.252 kb on + strand, within BWI76_RS21125at 4425.260 kb on - strand, within BWI76_RS21125at 4425.260 kb on - strand, within BWI76_RS21125at 4425.315 kb on - strand, within BWI76_RS21125at 4425.357 kb on - strand, within BWI76_RS21125at 4425.734 kb on - strand, within BWI76_RS21125at 4425.826 kb on + strand, within BWI76_RS21125at 4425.899 kb on - strand, within BWI76_RS21125at 4425.952 kb on - strand, within BWI76_RS21125at 4426.228 kb on + strandat 4426.228 kb on + strandat 4426.228 kb on + strandat 4426.236 kb on - strandat 4426.236 kb on - strandat 4426.405 kb on - strand, within BWI76_RS21130at 4426.454 kb on + strand, within BWI76_RS21130at 4426.459 kb on + strandat 4426.490 kb on + strandat 4427.115 kb on - strand, within BWI76_RS21140

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Per-strain Table

Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml
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4,423,980 + -1.2
4,424,282 + BWI76_RS21120 0.31 -0.5
4,424,282 + BWI76_RS21120 0.31 -2.3
4,424,282 + BWI76_RS21120 0.31 -1.2
4,424,290 - BWI76_RS21120 0.32 +0.7
4,424,290 - +0.4
4,424,292 - BWI76_RS21120 0.33 -1.1
4,424,384 - BWI76_RS21120 0.44 +0.9
4,424,529 + BWI76_RS21120 0.63 -0.5
4,424,681 - BWI76_RS21120 0.83 -1.1
4,425,034 - +2.0
4,425,226 + BWI76_RS21125 0.24 -0.4
4,425,252 + BWI76_RS21125 0.26 -1.2
4,425,260 - BWI76_RS21125 0.27 +0.4
4,425,260 - BWI76_RS21125 0.27 +0.7
4,425,315 - BWI76_RS21125 0.31 +3.0
4,425,357 - BWI76_RS21125 0.35 +1.5
4,425,734 - BWI76_RS21125 0.64 +0.4
4,425,826 + BWI76_RS21125 0.71 +0.8
4,425,899 - BWI76_RS21125 0.77 +0.0
4,425,952 - BWI76_RS21125 0.81 -0.6
4,426,228 + -1.2
4,426,228 + -0.6
4,426,228 + -0.1
4,426,236 - -2.1
4,426,236 - -1.2
4,426,405 - BWI76_RS21130 0.49 -1.1
4,426,454 + BWI76_RS21130 0.74 -0.8
4,426,459 + +0.4
4,426,490 + -0.2
4,427,115 - BWI76_RS21140 0.15 -0.7

Or see this region's nucleotide sequence