Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10795 and BWI76_RS10800 are separated by 427 nucleotides BWI76_RS10800 and BWI76_RS10805 are separated by 112 nucleotides BWI76_RS10805 and BWI76_RS10810 are separated by 69 nucleotides
BWI76_RS10795: BWI76_RS10795 - L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (from data) , at 2,277,440 to 2,281,402
_RS10795
BWI76_RS10800: BWI76_RS10800 - sodium:proline symporter, at 2,281,830 to 2,283,338
_RS10800
BWI76_RS10805: BWI76_RS10805 - hypothetical protein, at 2,283,451 to 2,283,885
_RS10805
BWI76_RS10810: BWI76_RS10810 - NupC family nucleoside transporter, at 2,283,955 to 2,285,139
_RS10810
Position (kb)
2281
2282
2283
2284 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2280.848 kb on + strand, within BWI76_RS10795 at 2280.922 kb on + strand, within BWI76_RS10795 at 2280.930 kb on - strand, within BWI76_RS10795 at 2281.167 kb on + strand at 2281.183 kb on - strand at 2281.375 kb on + strand at 2281.383 kb on - strand at 2281.383 kb on - strand at 2281.456 kb on - strand at 2281.693 kb on + strand at 2281.736 kb on - strand at 2281.736 kb on - strand at 2281.765 kb on - strand at 2281.833 kb on + strand at 2281.915 kb on - strand at 2281.951 kb on + strand at 2281.951 kb on + strand at 2281.951 kb on + strand at 2281.951 kb on + strand at 2281.951 kb on + strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2282.067 kb on - strand, within BWI76_RS10800 at 2282.070 kb on + strand, within BWI76_RS10800 at 2282.072 kb on - strand, within BWI76_RS10800 at 2282.073 kb on - strand, within BWI76_RS10800 at 2282.073 kb on - strand, within BWI76_RS10800 at 2282.085 kb on - strand, within BWI76_RS10800 at 2282.102 kb on + strand, within BWI76_RS10800 at 2282.102 kb on + strand, within BWI76_RS10800 at 2282.108 kb on + strand, within BWI76_RS10800 at 2282.195 kb on - strand, within BWI76_RS10800 at 2282.195 kb on - strand, within BWI76_RS10800 at 2282.270 kb on - strand, within BWI76_RS10800 at 2282.272 kb on + strand, within BWI76_RS10800 at 2282.280 kb on - strand, within BWI76_RS10800 at 2282.280 kb on - strand, within BWI76_RS10800 at 2282.282 kb on + strand, within BWI76_RS10800 at 2282.282 kb on + strand, within BWI76_RS10800 at 2282.285 kb on - strand, within BWI76_RS10800 at 2282.301 kb on - strand, within BWI76_RS10800 at 2282.349 kb on - strand, within BWI76_RS10800 at 2282.386 kb on - strand, within BWI76_RS10800 at 2282.394 kb on + strand, within BWI76_RS10800 at 2282.402 kb on - strand, within BWI76_RS10800 at 2282.413 kb on + strand, within BWI76_RS10800 at 2282.426 kb on - strand, within BWI76_RS10800 at 2282.640 kb on + strand, within BWI76_RS10800 at 2282.858 kb on - strand, within BWI76_RS10800 at 2282.895 kb on + strand, within BWI76_RS10800 at 2282.981 kb on + strand, within BWI76_RS10800 at 2282.989 kb on - strand, within BWI76_RS10800 at 2283.338 kb on + strand at 2283.514 kb on - strand, within BWI76_RS10805 at 2283.557 kb on - strand, within BWI76_RS10805 at 2283.810 kb on + strand, within BWI76_RS10805 at 2283.864 kb on + strand at 2283.928 kb on - strand at 2283.997 kb on - strand at 2284.002 kb on - strand at 2284.007 kb on - strand at 2284.015 kb on - strand at 2284.065 kb on - strand at 2284.072 kb on - strand at 2284.103 kb on - strand, within BWI76_RS10810 at 2284.149 kb on + strand, within BWI76_RS10810 at 2284.252 kb on - strand, within BWI76_RS10810 at 2284.278 kb on + strand, within BWI76_RS10810 at 2284.300 kb on - strand, within BWI76_RS10810
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 2,280,848 + BWI76_RS10795 0.86 -0.9 2,280,922 + BWI76_RS10795 0.88 -0.4 2,280,930 - BWI76_RS10795 0.88 -0.8 2,281,167 + +0.6 2,281,183 - -0.2 2,281,375 + -0.9 2,281,383 - +2.5 2,281,383 - -0.0 2,281,456 - +0.3 2,281,693 + -0.4 2,281,736 - +0.6 2,281,736 - +1.1 2,281,765 - +0.5 2,281,833 + -0.3 2,281,915 - +0.5 2,281,951 + -0.8 2,281,951 + +0.8 2,281,951 + +0.7 2,281,951 + -0.9 2,281,951 + +1.3 2,281,959 - +0.6 2,281,959 - +1.1 2,281,959 - -0.5 2,281,959 - -0.2 2,281,959 - +0.1 2,281,959 - +0.4 2,281,959 - +0.5 2,281,959 - +3.1 2,281,959 - +0.6 2,282,067 - BWI76_RS10800 0.16 -0.2 2,282,070 + BWI76_RS10800 0.16 +0.1 2,282,072 - BWI76_RS10800 0.16 -0.5 2,282,073 - BWI76_RS10800 0.16 -0.0 2,282,073 - BWI76_RS10800 0.16 +0.4 2,282,085 - BWI76_RS10800 0.17 -0.1 2,282,102 + BWI76_RS10800 0.18 -1.2 2,282,102 + BWI76_RS10800 0.18 +2.6 2,282,108 + BWI76_RS10800 0.18 -0.0 2,282,195 - BWI76_RS10800 0.24 +1.8 2,282,195 - BWI76_RS10800 0.24 +0.8 2,282,270 - BWI76_RS10800 0.29 -0.5 2,282,272 + BWI76_RS10800 0.29 +0.6 2,282,280 - BWI76_RS10800 0.30 -0.1 2,282,280 - BWI76_RS10800 0.30 +0.5 2,282,282 + BWI76_RS10800 0.30 +1.3 2,282,282 + BWI76_RS10800 0.30 +0.9 2,282,285 - BWI76_RS10800 0.30 +0.8 2,282,301 - BWI76_RS10800 0.31 -0.0 2,282,349 - BWI76_RS10800 0.34 +0.3 2,282,386 - BWI76_RS10800 0.37 +0.9 2,282,394 + BWI76_RS10800 0.37 +0.9 2,282,402 - BWI76_RS10800 0.38 -0.7 2,282,413 + BWI76_RS10800 0.39 +0.6 2,282,426 - BWI76_RS10800 0.39 -0.1 2,282,640 + BWI76_RS10800 0.54 -0.1 2,282,858 - BWI76_RS10800 0.68 +2.2 2,282,895 + BWI76_RS10800 0.71 +0.4 2,282,981 + BWI76_RS10800 0.76 +2.8 2,282,989 - BWI76_RS10800 0.77 +0.4 2,283,338 + +1.3 2,283,514 - BWI76_RS10805 0.14 -1.5 2,283,557 - BWI76_RS10805 0.24 +0.7 2,283,810 + BWI76_RS10805 0.83 -0.4 2,283,864 + -0.4 2,283,928 - +0.1 2,283,997 - +1.5 2,284,002 - -0.5 2,284,007 - -0.5 2,284,015 - +1.0 2,284,065 - -0.6 2,284,072 - +0.7 2,284,103 - BWI76_RS10810 0.12 +1.7 2,284,149 + BWI76_RS10810 0.16 -0.6 2,284,252 - BWI76_RS10810 0.25 -0.5 2,284,278 + BWI76_RS10810 0.27 -0.2 2,284,300 - BWI76_RS10810 0.29 +1.4
Or see this region's nucleotide sequence