Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10385 and BWI76_RS10390 are separated by 4 nucleotides BWI76_RS10390 and BWI76_RS10395 are separated by 518 nucleotides
BWI76_RS10385: BWI76_RS10385 - MmgE/PrpD family protein, at 2,198,350 to 2,199,759
_RS10385
BWI76_RS10390: BWI76_RS10390 - amino acid ABC transporter, at 2,199,764 to 2,200,588
_RS10390
BWI76_RS10395: BWI76_RS10395 - cold-shock protein, at 2,201,107 to 2,201,319
_RS10395
Position (kb)
2199
2200
2201 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2198.832 kb on - strand, within BWI76_RS10385 at 2199.436 kb on - strand, within BWI76_RS10385 at 2199.577 kb on + strand, within BWI76_RS10385 at 2199.805 kb on + strand at 2199.813 kb on - strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.828 kb on - strand at 2199.828 kb on - strand at 2199.828 kb on - strand at 2199.828 kb on - strand at 2200.149 kb on - strand, within BWI76_RS10390 at 2200.233 kb on + strand, within BWI76_RS10390 at 2200.235 kb on - strand, within BWI76_RS10390 at 2200.299 kb on + strand, within BWI76_RS10390 at 2200.307 kb on - strand, within BWI76_RS10390 at 2200.307 kb on - strand, within BWI76_RS10390 at 2200.307 kb on - strand, within BWI76_RS10390 at 2200.337 kb on + strand, within BWI76_RS10390 at 2200.341 kb on - strand, within BWI76_RS10390 at 2200.371 kb on - strand, within BWI76_RS10390 at 2200.387 kb on + strand, within BWI76_RS10390 at 2200.398 kb on + strand, within BWI76_RS10390 at 2200.399 kb on + strand at 2200.407 kb on - strand, within BWI76_RS10390 at 2200.408 kb on - strand, within BWI76_RS10390 at 2200.408 kb on - strand, within BWI76_RS10390 at 2200.413 kb on - strand, within BWI76_RS10390 at 2200.413 kb on - strand, within BWI76_RS10390 at 2200.438 kb on - strand, within BWI76_RS10390 at 2200.452 kb on + strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.465 kb on - strand, within BWI76_RS10390 at 2200.465 kb on - strand, within BWI76_RS10390 at 2200.465 kb on - strand at 2200.467 kb on + strand, within BWI76_RS10390 at 2200.470 kb on - strand, within BWI76_RS10390 at 2200.470 kb on - strand, within BWI76_RS10390 at 2200.475 kb on - strand, within BWI76_RS10390 at 2200.475 kb on - strand, within BWI76_RS10390 at 2200.514 kb on + strand at 2200.522 kb on - strand at 2200.548 kb on + strand at 2200.598 kb on - strand at 2200.650 kb on - strand at 2200.650 kb on - strand at 2200.694 kb on - strand at 2200.694 kb on - strand at 2200.784 kb on - strand at 2200.866 kb on + strand at 2200.875 kb on + strand at 2200.975 kb on + strand at 2200.975 kb on + strand at 2200.975 kb on + strand at 2200.975 kb on + strand at 2200.975 kb on + strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.067 kb on - strand at 2201.085 kb on - strand at 2201.114 kb on + strand at 2201.114 kb on + strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.127 kb on - strand at 2201.178 kb on - strand, within BWI76_RS10395 at 2201.183 kb on - strand, within BWI76_RS10395 at 2201.190 kb on - strand, within BWI76_RS10395 at 2201.248 kb on + strand at 2201.255 kb on - strand, within BWI76_RS10395 at 2201.256 kb on - strand, within BWI76_RS10395 at 2201.256 kb on - strand, within BWI76_RS10395 at 2201.270 kb on - strand at 2201.270 kb on - strand, within BWI76_RS10395 at 2201.270 kb on - strand, within BWI76_RS10395 at 2201.288 kb on - strand, within BWI76_RS10395 at 2201.344 kb on - strand at 2201.344 kb on - strand at 2201.344 kb on - strand at 2201.345 kb on - strand at 2201.345 kb on - strand at 2201.379 kb on - strand at 2201.425 kb on + strand at 2201.425 kb on + strand at 2201.427 kb on + strand at 2201.433 kb on - strand at 2201.471 kb on + strand at 2201.471 kb on + strand at 2201.550 kb on + strand at 2201.573 kb on + strand at 2201.584 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 2,198,832 - BWI76_RS10385 0.34 -0.6 2,199,436 - BWI76_RS10385 0.77 +0.3 2,199,577 + BWI76_RS10385 0.87 +0.1 2,199,805 + -1.2 2,199,813 - +0.0 2,199,820 + -0.2 2,199,820 + +0.1 2,199,820 + -0.7 2,199,820 + -1.2 2,199,820 + -0.6 2,199,820 + -0.1 2,199,828 - -0.7 2,199,828 - -0.9 2,199,828 - -0.4 2,199,828 - -0.3 2,200,149 - BWI76_RS10390 0.47 +0.8 2,200,233 + BWI76_RS10390 0.57 -0.5 2,200,235 - BWI76_RS10390 0.57 -0.9 2,200,299 + BWI76_RS10390 0.65 +0.1 2,200,307 - BWI76_RS10390 0.66 -0.8 2,200,307 - BWI76_RS10390 0.66 -0.2 2,200,307 - BWI76_RS10390 0.66 +0.7 2,200,337 + BWI76_RS10390 0.69 -0.0 2,200,341 - BWI76_RS10390 0.70 +1.0 2,200,371 - BWI76_RS10390 0.74 -0.2 2,200,387 + BWI76_RS10390 0.76 +0.6 2,200,398 + BWI76_RS10390 0.77 -1.5 2,200,399 + +2.4 2,200,407 - BWI76_RS10390 0.78 -0.6 2,200,408 - BWI76_RS10390 0.78 +0.6 2,200,408 - BWI76_RS10390 0.78 +0.8 2,200,413 - BWI76_RS10390 0.79 -0.9 2,200,413 - BWI76_RS10390 0.79 -0.4 2,200,438 - BWI76_RS10390 0.82 -0.8 2,200,452 + BWI76_RS10390 0.83 +0.4 2,200,460 - BWI76_RS10390 0.84 +0.6 2,200,460 - BWI76_RS10390 0.84 +0.3 2,200,460 - BWI76_RS10390 0.84 +0.2 2,200,460 - BWI76_RS10390 0.84 -1.7 2,200,460 - BWI76_RS10390 0.84 -0.5 2,200,460 - BWI76_RS10390 0.84 -0.4 2,200,460 - BWI76_RS10390 0.84 -0.4 2,200,465 - BWI76_RS10390 0.85 +0.1 2,200,465 - BWI76_RS10390 0.85 +0.1 2,200,465 - +2.4 2,200,467 + BWI76_RS10390 0.85 -0.3 2,200,470 - BWI76_RS10390 0.86 -0.3 2,200,470 - BWI76_RS10390 0.86 +0.3 2,200,475 - BWI76_RS10390 0.86 +0.6 2,200,475 - BWI76_RS10390 0.86 +0.3 2,200,514 + +0.4 2,200,522 - +1.4 2,200,548 + +0.8 2,200,598 - -0.3 2,200,650 - -1.1 2,200,650 - -0.2 2,200,694 - -0.2 2,200,694 - -1.3 2,200,784 - +0.2 2,200,866 + +0.6 2,200,875 + +0.1 2,200,975 + -1.1 2,200,975 + -0.2 2,200,975 + -0.2 2,200,975 + -0.9 2,200,975 + -0.5 2,201,056 - -0.6 2,201,056 - -0.2 2,201,056 - +0.3 2,201,067 - -0.3 2,201,085 - -0.1 2,201,114 + +1.1 2,201,114 + +1.2 2,201,122 - +2.4 2,201,122 - -1.1 2,201,122 - -0.3 2,201,122 - -0.9 2,201,122 - -0.3 2,201,122 - -0.2 2,201,122 - -1.3 2,201,122 - -1.8 2,201,122 - +0.5 2,201,122 - +0.1 2,201,122 - -1.1 2,201,122 - -0.7 2,201,122 - +0.9 2,201,122 - -0.4 2,201,127 - -0.8 2,201,178 - BWI76_RS10395 0.33 +0.4 2,201,183 - BWI76_RS10395 0.36 +0.3 2,201,190 - BWI76_RS10395 0.39 -0.6 2,201,248 + +1.8 2,201,255 - BWI76_RS10395 0.69 -0.3 2,201,256 - BWI76_RS10395 0.70 -1.9 2,201,256 - BWI76_RS10395 0.70 -0.2 2,201,270 - +0.8 2,201,270 - BWI76_RS10395 0.77 +1.8 2,201,270 - BWI76_RS10395 0.77 -1.0 2,201,288 - BWI76_RS10395 0.85 -0.5 2,201,344 - +0.2 2,201,344 - -1.0 2,201,344 - -1.2 2,201,345 - +0.8 2,201,345 - -0.2 2,201,379 - -1.8 2,201,425 + -0.4 2,201,425 + +1.8 2,201,427 + +2.4 2,201,433 - +0.2 2,201,471 + +0.2 2,201,471 + +0.1 2,201,550 + -0.1 2,201,573 + -1.3 2,201,584 + +3.4
Or see this region's nucleotide sequence