Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03415 and BWI76_RS03420 are separated by 136 nucleotides BWI76_RS03420 and BWI76_RS03425 overlap by 14 nucleotides BWI76_RS03425 and BWI76_RS03430 overlap by 7 nucleotides BWI76_RS03430 and BWI76_RS03435 are separated by 23 nucleotides
BWI76_RS03415: BWI76_RS03415 - LacI family transcriptional regulator, at 703,052 to 704,080
_RS03415
BWI76_RS03420: BWI76_RS03420 - hypothetical protein, at 704,217 to 704,648
_RS03420
BWI76_RS03425: BWI76_RS03425 - dihydrodipicolinate synthase family protein, at 704,635 to 705,546
_RS03425
BWI76_RS03430: BWI76_RS03430 - short-chain dehydrogenase, at 705,540 to 706,367
_RS03430
BWI76_RS03435: BWI76_RS03435 - mandelate racemase/muconate lactonizing protein, at 706,391 to 707,494
_RS03435
Position (kb)
704
705
706 Strain fitness (log2 ratio)
-2
-1
0
1 at 703.805 kb on + strand, within BWI76_RS03415 at 703.953 kb on + strand, within BWI76_RS03415 at 704.006 kb on - strand at 704.034 kb on + strand at 704.042 kb on - strand at 704.208 kb on - strand at 704.243 kb on - strand at 704.626 kb on - strand at 704.626 kb on - strand at 704.673 kb on + strand at 704.862 kb on + strand, within BWI76_RS03425 at 704.912 kb on - strand, within BWI76_RS03425 at 705.065 kb on + strand, within BWI76_RS03425 at 705.184 kb on - strand, within BWI76_RS03425 at 705.249 kb on - strand, within BWI76_RS03425 at 705.262 kb on - strand, within BWI76_RS03425 at 705.420 kb on - strand, within BWI76_RS03425 at 705.662 kb on + strand, within BWI76_RS03430 at 705.698 kb on + strand, within BWI76_RS03430 at 706.000 kb on - strand, within BWI76_RS03430 at 706.062 kb on + strand, within BWI76_RS03430 at 706.065 kb on - strand, within BWI76_RS03430 at 706.128 kb on + strand, within BWI76_RS03430 at 706.187 kb on - strand, within BWI76_RS03430 at 706.280 kb on - strand, within BWI76_RS03430 at 706.290 kb on - strand at 706.305 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 703,805 + BWI76_RS03415 0.73 +0.2 703,953 + BWI76_RS03415 0.88 -2.2 704,006 - -0.2 704,034 + -1.6 704,042 - +0.2 704,208 - +0.2 704,243 - +0.5 704,626 - -0.9 704,626 - -0.7 704,673 + -0.8 704,862 + BWI76_RS03425 0.25 -2.5 704,912 - BWI76_RS03425 0.30 -0.2 705,065 + BWI76_RS03425 0.47 -1.9 705,184 - BWI76_RS03425 0.60 -1.0 705,249 - BWI76_RS03425 0.67 -0.6 705,262 - BWI76_RS03425 0.69 -0.9 705,420 - BWI76_RS03425 0.86 -0.3 705,662 + BWI76_RS03430 0.15 +0.1 705,698 + BWI76_RS03430 0.19 +0.7 706,000 - BWI76_RS03430 0.56 -1.4 706,062 + BWI76_RS03430 0.63 -0.1 706,065 - BWI76_RS03430 0.63 -0.4 706,128 + BWI76_RS03430 0.71 +0.9 706,187 - BWI76_RS03430 0.78 -1.0 706,280 - BWI76_RS03430 0.89 -0.3 706,290 - -0.3 706,305 - -0.4
Or see this region's nucleotide sequence