Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01640 and BWI76_RS01645 are separated by 54 nucleotides BWI76_RS01645 and BWI76_RS01650 are separated by 99 nucleotides BWI76_RS01650 and BWI76_RS01655 are separated by 156 nucleotides
BWI76_RS01640: BWI76_RS01640 - 23S ribosomal RNA, at 331,939 to 334,865
_RS01640
BWI76_RS01645: BWI76_RS01645 - 5S ribosomal RNA, at 334,920 to 335,035
_RS01645
BWI76_RS01650: BWI76_RS01650 - acetyltransferase, at 335,135 to 335,572
_RS01650
BWI76_RS01655: BWI76_RS01655 - homoserine O-succinyltransferase, at 335,729 to 336,658
_RS01655
Position (kb) 335
336 Strain fitness (log2 ratio) -1
0
1 at 335.186 kb on - strand, within BWI76_RS01650 at 335.203 kb on + strand, within BWI76_RS01650 at 335.395 kb on - strand, within BWI76_RS01650 at 335.632 kb on + strand at 335.640 kb on - strand at 335.705 kb on - strand at 335.918 kb on - strand, within BWI76_RS01655 at 336.033 kb on + strand, within BWI76_RS01655 at 336.115 kb on - strand, within BWI76_RS01655 at 336.115 kb on - strand, within BWI76_RS01655 at 336.135 kb on + strand, within BWI76_RS01655 at 336.143 kb on - strand, within BWI76_RS01655 at 336.447 kb on + strand, within BWI76_RS01655 at 336.447 kb on + strand, within BWI76_RS01655 at 336.455 kb on - strand, within BWI76_RS01655
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 335,186 - BWI76_RS01650 0.12 -1.7 335,203 + BWI76_RS01650 0.16 -1.5 335,395 - BWI76_RS01650 0.59 -0.6 335,632 + -0.5 335,640 - +0.0 335,705 - -0.5 335,918 - BWI76_RS01655 0.20 +0.2 336,033 + BWI76_RS01655 0.33 +1.0 336,115 - BWI76_RS01655 0.42 -1.2 336,115 - BWI76_RS01655 0.42 -1.4 336,135 + BWI76_RS01655 0.44 -1.3 336,143 - BWI76_RS01655 0.45 -0.5 336,447 + BWI76_RS01655 0.77 -0.4 336,447 + BWI76_RS01655 0.77 -0.2 336,455 - BWI76_RS01655 0.78 +0.6
Or see this region's nucleotide sequence