Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01065

Experiment: Paraquat dichloride 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01060 and BWI76_RS01065 are separated by 134 nucleotidesBWI76_RS01065 and BWI76_RS01070 are separated by 341 nucleotides BWI76_RS01060: BWI76_RS01060 - guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase, at 216,812 to 218,311 _RS01060 BWI76_RS01065: BWI76_RS01065 - ATP-dependent RNA helicase RhlB, at 218,446 to 219,711 _RS01065 BWI76_RS01070: BWI76_RS01070 - thiol reductase thioredoxin, at 220,053 to 220,382 _RS01070 Position (kb) 218 219 220Strain fitness (log2 ratio) -2 -1 0 1 2at 217.717 kb on + strand, within BWI76_RS01060at 217.720 kb on - strand, within BWI76_RS01060at 217.835 kb on - strand, within BWI76_RS01060at 217.993 kb on - strand, within BWI76_RS01060at 218.297 kb on - strandat 218.299 kb on + strandat 218.321 kb on - strandat 218.332 kb on + strandat 218.340 kb on - strandat 218.448 kb on + strandat 218.451 kb on - strandat 218.533 kb on - strandat 218.533 kb on - strandat 218.965 kb on + strand, within BWI76_RS01065at 218.966 kb on + strand, within BWI76_RS01065at 219.065 kb on + strand, within BWI76_RS01065at 219.124 kb on - strand, within BWI76_RS01065at 219.124 kb on - strand, within BWI76_RS01065at 219.254 kb on - strand, within BWI76_RS01065at 219.323 kb on + strand, within BWI76_RS01065at 219.377 kb on - strand, within BWI76_RS01065at 219.742 kb on - strandat 219.863 kb on + strandat 219.901 kb on + strandat 220.103 kb on + strand, within BWI76_RS01070at 220.103 kb on + strand, within BWI76_RS01070at 220.103 kb on + strand, within BWI76_RS01070at 220.111 kb on - strand, within BWI76_RS01070at 220.125 kb on - strand, within BWI76_RS01070at 220.125 kb on - strand, within BWI76_RS01070at 220.155 kb on - strand, within BWI76_RS01070at 220.226 kb on + strand, within BWI76_RS01070at 220.337 kb on + strand, within BWI76_RS01070at 220.344 kb on + strand, within BWI76_RS01070at 220.345 kb on - strand, within BWI76_RS01070at 220.345 kb on - strand, within BWI76_RS01070at 220.414 kb on - strandat 220.433 kb on - strandat 220.448 kb on + strandat 220.504 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml
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217,717 + BWI76_RS01060 0.60 -0.3
217,720 - BWI76_RS01060 0.61 +0.1
217,835 - BWI76_RS01060 0.68 -0.3
217,993 - BWI76_RS01060 0.79 -0.8
218,297 - -2.2
218,299 + +0.4
218,321 - +0.4
218,332 + +0.7
218,340 - -0.1
218,448 + +0.9
218,451 - +0.5
218,533 - +1.1
218,533 - +0.9
218,965 + BWI76_RS01065 0.41 +1.8
218,966 + BWI76_RS01065 0.41 +1.6
219,065 + BWI76_RS01065 0.49 +1.0
219,124 - BWI76_RS01065 0.54 +1.7
219,124 - BWI76_RS01065 0.54 +2.3
219,254 - BWI76_RS01065 0.64 -0.7
219,323 + BWI76_RS01065 0.69 +1.7
219,377 - BWI76_RS01065 0.74 +1.7
219,742 - +0.1
219,863 + +0.1
219,901 + -0.7
220,103 + BWI76_RS01070 0.15 +0.7
220,103 + BWI76_RS01070 0.15 +0.4
220,103 + BWI76_RS01070 0.15 +1.3
220,111 - BWI76_RS01070 0.18 +2.6
220,125 - BWI76_RS01070 0.22 +2.7
220,125 - BWI76_RS01070 0.22 +1.4
220,155 - BWI76_RS01070 0.31 +1.6
220,226 + BWI76_RS01070 0.52 +0.3
220,337 + BWI76_RS01070 0.86 +2.1
220,344 + BWI76_RS01070 0.88 -0.0
220,345 - BWI76_RS01070 0.88 +0.5
220,345 - BWI76_RS01070 0.88 +0.5
220,414 - +0.6
220,433 - +1.1
220,448 + -1.7
220,504 + -2.2

Or see this region's nucleotide sequence