Experiment: Def Vancomycin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0088 and Psest_0089 overlap by 10 nucleotides Psest_0089 and Psest_0090 are separated by 98 nucleotides Psest_0090 and Psest_0091 overlap by 11 nucleotides Psest_0091 and Psest_0092 are separated by 11 nucleotides
Psest_0088: Psest_0088 - phosphonate metabolism protein PhnP, at 89,559 to 90,308
_0088
Psest_0089: Psest_0089 - phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN, at 90,299 to 90,853
_0089
Psest_0090: Psest_0090 - phosphonate metabolism protein PhnM, at 90,952 to 92,097
_0090
Psest_0091: Psest_0091 - phosphonate C-P lyase system protein PhnL, at 92,087 to 92,791
_0091
Psest_0092: Psest_0092 - phosphonate C-P lyase system protein PhnK, at 92,803 to 93,615
_0092
Position (kb)
90
91
92
93 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 89.983 kb on + strand, within Psest_0088 at 89.983 kb on + strand, within Psest_0088 at 89.984 kb on - strand, within Psest_0088 at 89.984 kb on - strand, within Psest_0088 at 89.984 kb on - strand, within Psest_0088 at 89.984 kb on - strand, within Psest_0088 at 90.259 kb on + strand at 90.259 kb on + strand at 90.384 kb on + strand, within Psest_0089 at 90.384 kb on + strand, within Psest_0089 at 90.384 kb on + strand, within Psest_0089 at 90.384 kb on + strand, within Psest_0089 at 90.384 kb on + strand, within Psest_0089 at 90.384 kb on + strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.549 kb on + strand, within Psest_0089 at 90.621 kb on + strand, within Psest_0089 at 90.835 kb on - strand at 90.835 kb on - strand at 90.898 kb on + strand at 90.898 kb on + strand at 90.898 kb on + strand at 90.898 kb on + strand at 90.898 kb on + strand at 90.898 kb on + strand at 90.898 kb on + strand at 90.898 kb on + strand at 90.899 kb on - strand at 90.899 kb on - strand at 91.010 kb on - strand at 91.010 kb on - strand at 91.010 kb on - strand at 91.010 kb on - strand at 91.010 kb on - strand at 91.182 kb on - strand, within Psest_0090 at 91.374 kb on + strand, within Psest_0090 at 91.529 kb on + strand, within Psest_0090 at 91.529 kb on + strand, within Psest_0090 at 91.529 kb on + strand, within Psest_0090 at 91.530 kb on - strand, within Psest_0090 at 91.530 kb on - strand, within Psest_0090 at 91.530 kb on - strand, within Psest_0090 at 91.530 kb on - strand, within Psest_0090 at 91.530 kb on - strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.541 kb on + strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.542 kb on - strand, within Psest_0090 at 91.544 kb on + strand, within Psest_0090 at 91.545 kb on - strand, within Psest_0090 at 91.554 kb on - strand, within Psest_0090 at 92.080 kb on + strand at 92.080 kb on + strand at 92.080 kb on + strand at 92.081 kb on - strand at 92.081 kb on - strand at 92.503 kb on - strand, within Psest_0091 at 92.531 kb on + strand, within Psest_0091 at 92.531 kb on + strand, within Psest_0091 at 92.532 kb on - strand, within Psest_0091 at 92.532 kb on - strand, within Psest_0091 at 92.610 kb on + strand, within Psest_0091 at 92.610 kb on + strand, within Psest_0091 at 92.610 kb on + strand, within Psest_0091 at 92.610 kb on + strand, within Psest_0091 at 92.610 kb on + strand, within Psest_0091 at 92.610 kb on + strand, within Psest_0091 at 92.611 kb on - strand, within Psest_0091 at 92.611 kb on - strand, within Psest_0091 at 92.611 kb on - strand, within Psest_0091 at 92.611 kb on - strand, within Psest_0091 at 92.620 kb on - strand, within Psest_0091 at 92.838 kb on + strand at 92.839 kb on - strand at 92.839 kb on - strand at 92.840 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Vancomycin 0.008 mg/ml remove 89,983 + Psest_0088 0.57 +1.9 89,983 + Psest_0088 0.57 +1.3 89,984 - Psest_0088 0.57 +0.8 89,984 - Psest_0088 0.57 +0.3 89,984 - Psest_0088 0.57 +0.2 89,984 - Psest_0088 0.57 +0.9 90,259 + -0.7 90,259 + +1.9 90,384 + Psest_0089 0.15 -0.7 90,384 + Psest_0089 0.15 -2.0 90,384 + Psest_0089 0.15 -0.7 90,384 + Psest_0089 0.15 +1.2 90,384 + Psest_0089 0.15 +1.5 90,384 + Psest_0089 0.15 -0.1 90,385 - Psest_0089 0.15 -0.7 90,385 - Psest_0089 0.15 +0.7 90,385 - Psest_0089 0.15 +0.9 90,385 - Psest_0089 0.15 +1.2 90,385 - Psest_0089 0.15 +1.9 90,385 - Psest_0089 0.15 +1.3 90,385 - Psest_0089 0.15 +0.6 90,385 - Psest_0089 0.15 +0.3 90,549 + Psest_0089 0.45 +1.7 90,621 + Psest_0089 0.58 +1.5 90,835 - +1.9 90,835 - +0.8 90,898 + +1.9 90,898 + +2.2 90,898 + -0.1 90,898 + +1.9 90,898 + -0.1 90,898 + -0.7 90,898 + +1.9 90,898 + +1.9 90,899 - +0.3 90,899 - +1.9 91,010 - +1.3 91,010 - +0.3 91,010 - +3.5 91,010 - -0.1 91,010 - +0.3 91,182 - Psest_0090 0.20 +0.9 91,374 + Psest_0090 0.37 +0.9 91,529 + Psest_0090 0.50 +1.3 91,529 + Psest_0090 0.50 -0.4 91,529 + Psest_0090 0.50 +0.3 91,530 - Psest_0090 0.50 +0.3 91,530 - Psest_0090 0.50 -0.9 91,530 - Psest_0090 0.50 +0.3 91,530 - Psest_0090 0.50 -1.8 91,530 - Psest_0090 0.50 +0.9 91,541 + Psest_0090 0.51 +0.1 91,541 + Psest_0090 0.51 -0.1 91,541 + Psest_0090 0.51 +0.9 91,541 + Psest_0090 0.51 +0.6 91,541 + Psest_0090 0.51 +1.9 91,541 + Psest_0090 0.51 +1.2 91,541 + Psest_0090 0.51 -0.7 91,541 + Psest_0090 0.51 -0.4 91,541 + Psest_0090 0.51 +0.3 91,541 + Psest_0090 0.51 -1.0 91,541 + Psest_0090 0.51 -0.3 91,541 + Psest_0090 0.51 -1.1 91,541 + Psest_0090 0.51 -1.6 91,541 + Psest_0090 0.51 +1.9 91,541 + Psest_0090 0.51 -0.1 91,541 + Psest_0090 0.51 -0.4 91,541 + Psest_0090 0.51 -0.7 91,541 + Psest_0090 0.51 +1.9 91,541 + Psest_0090 0.51 -0.2 91,541 + Psest_0090 0.51 +1.4 91,541 + Psest_0090 0.51 +0.3 91,541 + Psest_0090 0.51 +0.9 91,541 + Psest_0090 0.51 +0.9 91,541 + Psest_0090 0.51 -0.4 91,541 + Psest_0090 0.51 -0.1 91,542 - Psest_0090 0.51 -0.9 91,542 - Psest_0090 0.51 +1.9 91,542 - Psest_0090 0.51 +0.3 91,542 - Psest_0090 0.51 +1.9 91,542 - Psest_0090 0.51 +1.2 91,542 - Psest_0090 0.51 -0.4 91,542 - Psest_0090 0.51 -0.7 91,542 - Psest_0090 0.51 +0.3 91,542 - Psest_0090 0.51 -0.4 91,542 - Psest_0090 0.51 +1.9 91,542 - Psest_0090 0.51 +1.9 91,542 - Psest_0090 0.51 +0.3 91,542 - Psest_0090 0.51 -0.7 91,542 - Psest_0090 0.51 +0.6 91,542 - Psest_0090 0.51 -0.1 91,542 - Psest_0090 0.51 +3.5 91,542 - Psest_0090 0.51 +0.6 91,542 - Psest_0090 0.51 +1.3 91,542 - Psest_0090 0.51 +0.9 91,542 - Psest_0090 0.51 +0.2 91,542 - Psest_0090 0.51 -0.4 91,542 - Psest_0090 0.51 +1.9 91,542 - Psest_0090 0.51 -0.3 91,542 - Psest_0090 0.51 -0.7 91,542 - Psest_0090 0.51 +0.3 91,542 - Psest_0090 0.51 +1.9 91,542 - Psest_0090 0.51 +0.6 91,542 - Psest_0090 0.51 +0.3 91,542 - Psest_0090 0.51 +1.3 91,542 - Psest_0090 0.51 -0.1 91,542 - Psest_0090 0.51 +0.3 91,544 + Psest_0090 0.52 -0.7 91,545 - Psest_0090 0.52 +0.4 91,554 - Psest_0090 0.53 -0.9 92,080 + -0.7 92,080 + +1.9 92,080 + +0.9 92,081 - -1.3 92,081 - -0.4 92,503 - Psest_0091 0.59 +1.9 92,531 + Psest_0091 0.63 +1.9 92,531 + Psest_0091 0.63 +1.9 92,532 - Psest_0091 0.63 +0.3 92,532 - Psest_0091 0.63 -2.8 92,610 + Psest_0091 0.74 -0.1 92,610 + Psest_0091 0.74 -3.1 92,610 + Psest_0091 0.74 -0.1 92,610 + Psest_0091 0.74 +1.3 92,610 + Psest_0091 0.74 +0.6 92,610 + Psest_0091 0.74 +0.6 92,611 - Psest_0091 0.74 +0.7 92,611 - Psest_0091 0.74 +0.9 92,611 - Psest_0091 0.74 +1.5 92,611 - Psest_0091 0.74 +1.9 92,620 - Psest_0091 0.76 +0.9 92,838 + +0.9 92,839 - -0.1 92,839 - -0.7 92,840 + -0.9
Or see this region's nucleotide sequence