Experiment: Def Vancomycin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0672 and Psest_0673 are separated by 204 nucleotides Psest_0673 and Psest_0674 are separated by 107 nucleotides Psest_0674 and Psest_0675 are separated by 1 nucleotides
Psest_0672: Psest_0672 - Alcohol dehydrogenase, class IV, at 733,496 to 734,644
_0672
Psest_0673: Psest_0673 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase, at 734,849 to 736,198
_0673
Psest_0674: Psest_0674 - polyprenyl P-hydroxybenzoate and phenylacrylic acid decarboxylases, at 736,306 to 736,935
_0674
Psest_0675: Psest_0675 - Predicted periplasmic lipoprotein, at 736,937 to 737,227
_0675
Position (kb) 734
735
736
737 Strain fitness (log2 ratio) -2
-1
0
1
2 at 733.866 kb on + strand, within Psest_0672 at 733.867 kb on - strand, within Psest_0672 at 733.867 kb on - strand, within Psest_0672 at 733.914 kb on + strand, within Psest_0672 at 733.914 kb on + strand, within Psest_0672 at 733.915 kb on - strand, within Psest_0672 at 733.915 kb on - strand, within Psest_0672 at 734.040 kb on + strand, within Psest_0672 at 734.040 kb on + strand, within Psest_0672 at 734.040 kb on + strand, within Psest_0672 at 734.040 kb on + strand, within Psest_0672 at 734.040 kb on + strand, within Psest_0672 at 734.040 kb on + strand, within Psest_0672 at 734.040 kb on + strand, within Psest_0672 at 734.041 kb on - strand, within Psest_0672 at 734.041 kb on - strand, within Psest_0672 at 734.041 kb on - strand, within Psest_0672 at 734.041 kb on - strand, within Psest_0672 at 734.171 kb on + strand, within Psest_0672 at 734.289 kb on + strand, within Psest_0672 at 734.289 kb on + strand, within Psest_0672 at 734.300 kb on - strand, within Psest_0672 at 734.562 kb on + strand at 734.562 kb on + strand at 734.562 kb on + strand at 734.563 kb on - strand at 734.563 kb on - strand at 734.854 kb on + strand at 734.861 kb on - strand at 735.086 kb on + strand, within Psest_0673 at 735.086 kb on + strand, within Psest_0673 at 735.086 kb on + strand, within Psest_0673 at 735.087 kb on - strand, within Psest_0673 at 735.111 kb on - strand, within Psest_0673 at 735.332 kb on + strand, within Psest_0673 at 735.827 kb on + strand, within Psest_0673 at 735.828 kb on - strand, within Psest_0673 at 735.828 kb on - strand, within Psest_0673 at 735.828 kb on - strand, within Psest_0673 at 735.828 kb on - strand, within Psest_0673 at 735.828 kb on - strand, within Psest_0673 at 735.898 kb on + strand, within Psest_0673 at 735.898 kb on + strand, within Psest_0673 at 736.067 kb on - strand at 736.236 kb on + strand at 736.236 kb on + strand at 736.236 kb on + strand at 736.256 kb on + strand at 736.917 kb on + strand at 737.039 kb on + strand, within Psest_0675 at 737.039 kb on + strand, within Psest_0675 at 737.039 kb on + strand, within Psest_0675 at 737.039 kb on + strand, within Psest_0675 at 737.040 kb on - strand, within Psest_0675 at 737.040 kb on - strand, within Psest_0675 at 737.121 kb on - strand, within Psest_0675 at 737.121 kb on - strand, within Psest_0675
Per-strain Table
Position Strand Gene LocusTag Fraction Def Vancomycin 0.008 mg/ml remove 733,866 + Psest_0672 0.32 +0.2 733,867 - Psest_0672 0.32 -0.3 733,867 - Psest_0672 0.32 +0.2 733,914 + Psest_0672 0.36 +0.5 733,914 + Psest_0672 0.36 +0.7 733,915 - Psest_0672 0.36 -1.6 733,915 - Psest_0672 0.36 -1.1 734,040 + Psest_0672 0.47 -0.3 734,040 + Psest_0672 0.47 +1.5 734,040 + Psest_0672 0.47 -0.5 734,040 + Psest_0672 0.47 -0.3 734,040 + Psest_0672 0.47 -1.5 734,040 + Psest_0672 0.47 -0.9 734,040 + Psest_0672 0.47 +0.3 734,041 - Psest_0672 0.47 -0.2 734,041 - Psest_0672 0.47 +1.3 734,041 - Psest_0672 0.47 +0.9 734,041 - Psest_0672 0.47 +0.3 734,171 + Psest_0672 0.59 -0.9 734,289 + Psest_0672 0.69 +1.1 734,289 + Psest_0672 0.69 -0.2 734,300 - Psest_0672 0.70 -0.6 734,562 + +0.3 734,562 + -1.9 734,562 + +0.4 734,563 - -0.8 734,563 - -0.2 734,854 + +1.1 734,861 - -0.3 735,086 + Psest_0673 0.18 -1.7 735,086 + Psest_0673 0.18 -0.6 735,086 + Psest_0673 0.18 -0.8 735,087 - Psest_0673 0.18 -0.6 735,111 - Psest_0673 0.19 +0.4 735,332 + Psest_0673 0.36 -0.9 735,827 + Psest_0673 0.72 -0.3 735,828 - Psest_0673 0.73 -2.1 735,828 - Psest_0673 0.73 -0.6 735,828 - Psest_0673 0.73 -1.3 735,828 - Psest_0673 0.73 -0.9 735,828 - Psest_0673 0.73 -0.9 735,898 + Psest_0673 0.78 +0.1 735,898 + Psest_0673 0.78 -0.6 736,067 - -1.1 736,236 + +1.7 736,236 + -0.3 736,236 + -0.9 736,256 + -1.2 736,917 + +1.1 737,039 + Psest_0675 0.35 +2.0 737,039 + Psest_0675 0.35 +1.4 737,039 + Psest_0675 0.35 +0.1 737,039 + Psest_0675 0.35 -0.6 737,040 - Psest_0675 0.35 -2.1 737,040 - Psest_0675 0.35 -0.2 737,121 - Psest_0675 0.63 -0.7 737,121 - Psest_0675 0.63 +0.4
Or see this region's nucleotide sequence